Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Дек. 11, 2024
Type VI Secretion Systems (T6SS), widely distributed in Gram-negative bacteria, contribute to interbacterial competition and pathogenesis through the translocation of effector proteins target cells.
Язык: Английский
Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(7), С. 7447 - 7472
Опубликована: Июль 14, 2024
Even with the intensive efforts by public health programs to control and prevent it, non-typhoidal Salmonella (NTS) infection remains an important challenge. It is responsible for approximately 150 million illnesses 60,000 deaths worldwide annually. NTS poses significant risks high rates of morbidity mortality, leading potential short- long-term complications. There growing concern among authorities about increasing incidence antimicrobial resistance, multidrug resistance totaling 22.6% in Europe, highlighting urgent need new therapeutic approaches. Our review aims provide a comprehensive overview infection. We outline molecular mechanisms involved pathogenesis infection, as well events invasive subsequent complications associated it. Given widespread implications our also presents global landscape including delve into underlying driving this resistance. The rising antibiotic frequently lead treatment failures, emphasizing importance investigating alternative options. Therefore, we explore therapies that could offer promising approaches treating infections.
Язык: Английский
Процитировано
9
Опубликована: Март 14, 2025
Salmonella Dublin is a host-adapted, invasive non-typhoidal (iNTS) serovar that causes bloodstream infections in humans and demonstrates increasing prevalence of antimicrobial resistance (AMR). Using global dataset 1,303 genomes, coupled with vitro assays, we examined the evolutionary, resistance, virulence characteristics S . Dublin. Our analysis revealed strong geographic associations between AMR profiles plasmid types, highly resistant isolates confined predominantly to North America, linked IncC plasmids co-encoding heavy metal resistance. By contrast, Australian were largely antimicrobial-susceptible, reflecting differing pressures. We identified two phylogenetically distinct lineages, ST10 ST74, small number harbouring novel hybrid encoding both mercuric Whereas lineage remains globally dominant, ST74 was less prevalent. exhibited unique genomic features including larger pan genome compared absence key loci SPI-19 which encodes type VI secretion system (T6SS). Despite these differences, displayed enhanced intracellular replication human macrophages induced pro-inflammatory responses ST10, suggesting alternative strategies may support systemic dissemination ST74. The Vi antigen absent all highlighting challenges for serotyping vaccine development, has implications current diagnostic control S. infections. Collectively, this study represents most comprehensive investigation date importantly, adaptations genotypes within same serovar, leading different epidemiological success. regional emergence evolution lineages highlights need understand divergence intra-serovar mechanisms impact development effective measures against important pathogen.
Язык: Английский
Процитировано
1
Trends in Microbiology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Serovars of Salmonella are significant bacterial pathogens and leading contributors to the global burden diarrhoeal disease. pathogenicity islands (SPIs) essential for survival success this genus, enabling colonisation, invasion, in hostile environments. While genomics has transformed efforts understand evolution, dissemination, antimicrobial resistance members, its use explore virulence determinants that contribute specific organisms severity infection remains varied. Here, we discuss importance SPIs evolution Salmonella, implications shift identification from molecular microbiology genomic-based approaches, examine current distribution prevalence large-scale datasets genomes.
Язык: Английский
Процитировано
0
Опубликована: Апрель 23, 2025
Salmonella Dublin is a host-adapted, invasive non-typhoidal (iNTS) serovar that causes bloodstream infections in humans and demonstrates increasing prevalence of antimicrobial resistance (AMR). Using global dataset 1,303 genomes, coupled with vitro assays, we examined the evolutionary, resistance, virulence characteristics S . Dublin. Our analysis revealed strong geographic associations between AMR profiles plasmid types, highly resistant isolates confined predominantly to North America, linked IncC plasmids co-encoding heavy metal resistance. By contrast, Australian were largely antimicrobial-susceptible, reflecting differing pressures. We identified two phylogenetically distinct lineages, ST10 ST74, small number harbouring novel hybrid encoding both mercuric Whereas lineage remains globally dominant, ST74 was less prevalent. exhibited unique genomic features including larger pan genome compared absence key loci SPI-19 which encodes type VI secretion system (T6SS). Despite these differences, displayed enhanced intracellular replication human macrophages induced pro-inflammatory responses ST10, suggesting alternative strategies may support systemic dissemination ST74. The Vi antigen absent all highlighting challenges for serotyping vaccine development, has implications current diagnostic control S. infections. Collectively, this study represents most comprehensive investigation date importantly, adaptations genotypes within same serovar, leading different epidemiological success. regional emergence evolution lineages highlights need understand divergence intra-serovar mechanisms impact development effective measures against important pathogen.
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Окт. 24, 2024
Abstract Salmonella enterica , a prominent foodborne pathogen, contributes significantly to global illnesses annually. This species exhibits significant genetic diversity, potentially impacting its infectivity, disease severity, and antimicrobial resistance. Whole genome sequencing (WGS) offers comprehensive insights that can be utilized for virulence assessment. However, existing bioinformatic tools studying have notable limitations. To address this gap, Virulence Database with non-redundant, list of putative factors was constructed. Two analysis tools, Factor Profile Assessment Comparison were developed. The former provides data on similarity the reference genes, e-value, bite score, while latter assesses presence/absence genes in isolates facilitates comparison profiles across multiple sequences. validate database associated WGS from 43,853 spanning 14 serovars extracted GenBank, previously generated our lab used. Overall, effectively facilitated assessment, enhancing understanding among serovars. public availability these resources will empower researchers assess comprehensively, which could inform strategies pathogen control risk evaluations human illnesses.
Язык: Английский
Процитировано
3
Infection and Drug Resistance, Год журнала: 2023, Номер Volume 16, С. 6911 - 6922
Опубликована: Окт. 1, 2023
Abstract: Bacteria communicate with their surroundings through diverse secretory systems, and the recently discovered Type VI Secretion System (T6SS) has gained significant attention. Klebsiella pneumoniae (K. pneumoniae) , an opportunistic pathogen known for causing severe infections in both hospital animal settings, possesses this intriguing T6SS. This system equips K. a formidable armory of protein-based weaponry, enabling delivery toxins into neighboring cells, thus granting substantial competitive advantage. Remarkably, T6SS also been associated pneumoniae’s ability to form biofilms acquire resistance against antibiotics. However, precise effects on functions remain inadequately studied, despite research efforts understand intricacies these mechanisms. comprehensive review aims provide overview current knowledge regarding biological regulatory mechanisms pneumoniae. Keywords: T6SS, competition, drug resistance, virulence
Язык: Английский
Процитировано
5
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Сен. 28, 2024
Bacterial warfare is a common and ancient phenomenon in nature, where bacterial species use strategies to inhibit the growth or kill competitors. This involves production deployment of antibacterial toxins that disrupt essential cellular processes target cells. Polymorphic comprise group offensive systems with modular structure featuring conserved N-terminal translocation domain fused diverse C-terminal toxin domains. The continuous arms race which bacteria acquire new immunity proteins promote increased adaptation their environment responsible for diversification this repertoire. Here, we deployed in-silico analyze 10,000 genomes identify domains secreted via type VI secretion system Salmonella . We identified manually curated 128 candidates, are widespread polymorphic detected vast array linked systems. In addition, 45 previously uncharacterized were identified. STox15 was among most frequent candidates found dataset selected in-depth characterization. an effector belonging NlpC/P60 papain-like fold superfamily permuted catalytic core typical lipid-targeting versions rather than peptidases amidases. Biochemical analysis recombinant protein lipidomics intoxicated Escherichia coli revealed displays phospholipase activity cleaving off acyl groups from phosphatidylglycerol phosphatidylethanolamine. work broadens our understanding provides first direct characterization biological conflicts.
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 30, 2024
ABSTRACT Salmonella Dublin is a host-adapted, invasive non-typhoidal (iNTS) serovar that causes bloodstream infections in humans and demonstrates increasing prevalence of antimicrobial resistance (AMR). Using global dataset 1,303 genomes, coupled with vitro assays, we examined the evolutionary, resistance, virulence characteristics S . Dublin. Our analysis revealed strong geographic associations between AMR profiles plasmid types, highly resistant isolates confined predominantly to North America, linked IncC plasmids co-encoding heavy metal resistance. By contrast, Australian were largely antimicrobial-susceptible, reflecting differing pressures. We identified two phylogenetically distinct lineages, ST10 ST74, small number harbouring novel hybrid encoding both mercuric Whereas lineage remains globally dominant, ST74 was less prevalent. exhibited unique genomic features including larger pan genome compared absence key loci SPI-19 which encodes type VI secretion system (T6SS). Despite these differences, displayed enhanced intracellular replication human macrophages induced pro-inflammatory responses ST10, suggesting alternative strategies may support systemic dissemination ST74. The Vi antigen absent all highlighting challenges for serotyping vaccine development, has implications current diagnostic control S. infections. Collectively, this study represents most comprehensive investigation date importantly, adaptations genotypes within same serovar, leading different epidemiological success. regional emergence evolution lineages highlights need understand divergence intra-serovar mechanisms impact development effective measures against important pathogen.
Язык: Английский
Процитировано
0
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Авг. 20, 2024
Язык: Английский
Процитировано
0
Опубликована: Ноя. 11, 2024
Salmonella Dublin is a host-adapted, invasive non-typhoidal (iNTS) serovar that causes bloodstream infections in humans and demonstrates increasing prevalence of antimicrobial resistance (AMR). Here, we characterised global population 1,303 S . isolates for virulence mechanisms using combined approach silico vitro analyses. We observed distinct populations Vi antigen-negative S. circulating different geographical regions, with the emergence North American cluster ∼60 years ago. Further, identified two lineages Australia, within which discovered novel hybrid plasmid encoding both AMR mercuric resistance, highlighting potential emerging virulent resistant lineages. These differences, addition to lack flagella represent adaptation may enhance survival pathogenicity, turn affect prevention control strategies future infections. Collectively, our study embodies one most comprehensive investigations highlights impact pathogen on health economic burdens experienced by sectors including livestock human health.
Язык: Английский
Процитировано
0