A comparison between full-length 16S rRNA Oxford nanopore sequencing and Illumina V3-V4 16S rRNA sequencing in head and neck cancer tissues

Archives of Microbiology, Год журнала: 2024, Номер 206(6)

Опубликована: Май 7, 2024

Describing the microbial community within tumour has been a key aspect in understanding pathophysiology of microenvironment. In head and neck cancer (HNC), most studies on tissue samples have only performed 16S rRNA short-read sequencing (SRS) V3-V5 region. SRS is mostly limited to genus level identification. this study, we compared full-length long-read (FL-ONT) from Oxford Nanopore Technology (ONT) V3-V4 Illumina (V3V4-Illumina) 26 HNC tissues. Further validation was also using culture-based methods 16 bacterial isolates obtained 4 patients MALDI-TOF MS. We observed similar alpha diversity indexes between FL-ONT V3V4-Illumina. However, beta-diversity significantly different techniques (PERMANOVA - R

Язык: Английский

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Causal Association Between Microbiome and Oral-Oropharyngeal Cancer: A Mendelian Randomization Study

International Dental Journal, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

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Exploring the role of oral bacteria in oral cancer: a narrative review

Discover Oncology, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 26, 2025

A growing body of research indicates that a wide range cancer types may correlate with human microbiome components. On the other hand, little is known about potential contribution oral microbiota to cancer. However, some components can stimulate different tumorigenic processes associated development In this line, two prevalent infections, Porphyromonas gingivalis, and Fusobacterium nucleatum increase tumor growth. The impact course illness through direct interactions major modifications toxicity responsiveness kinds therapy. Recent has demonstrated relationship between specific phylogenetic groupings results immunotherapy treatment for particular types. Conversely, there been recent upsurge in interest possibility using microbes treat At moment, species, such as Salmonella typhimurium Clostridium spp., are being explored possible vectors. Thus, understanding these microbial highlights importance maintaining healthy preventing cancers. From perspective, review will discuss role on cancers their application treatment/improvement.

Язык: Английский

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CD47 and thrombospondin-1 contribute to immune evasion by Porphyromonas gingivalis

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(47)

Опубликована: Ноя. 13, 2024

is a gram-negative anaerobic bacterium linked to periodontal disease. Remarkably,

Язык: Английский

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γδ17T Cells Aggravate Carcinogen-Induced Oral Squamous Cell Carcinoma

Journal of Dental Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Oral squamous cell carcinoma (OSCC) is a highly aggressive malignancy, with low 5-y survival rate and frequent local recurrence or metastasis. This study explores the role of γδT cells in development progression OSCC. cells, which exhibit innate adaptive immune characteristics, are known for their dual cancer, acting as anti- protumor agents depending on context. Using murine model OSCC induced by carcinogen 4-nitroquinoline-1-oxide (4NQO), adequately mimics human OSCC, we investigated impact tumor growth microenvironment. We first characterized tongue epithelium, primary site cancer this model. The results indicate that predominantly Vγ6+ subset, expanding postnatally microbiota-dependent manner. Upon 4NQO administration, depletion did not significantly alter kinetics but result reduction size number, suggesting promoting growth. Interestingly, absence IL-17, key cytokine produced also resulted reduced volume without affecting disease progression, corroborating these Further analysis revealed IL-17-producing facilitate angiogenesis within microenvironment expression angiogenic factors. Of note, while treatment increased oral microbial load altered its composition, IL-17 deficiency affect microbiota, indicating effects independent changes. highlights pathologic particularly through angiogenesis. underscores importance need further research into therapeutic strategies targeting cells.

Язык: Английский

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Radiation-induced changes in salivary metabolite profile and pathways in head and neck cancer patients

Clinical Oral Investigations, Год журнала: 2025, Номер 29(3)

Опубликована: Фев. 21, 2025

Язык: Английский

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A Potential Link Between Oral Microbiota and Female Reproductive Health

Microorganisms, Год журнала: 2025, Номер 13(3), С. 619 - 619

Опубликована: Март 7, 2025

Oral cavity dysbiosis is associated with numerous inflammatory diseases, including diabetes, bowel and periodontal disease. Changes in the oral microenvironment lead to bidirectional interactions between pathogens individual host systems, which may induce systemic inflammation. There increasing evidence linking condition of most common causes female infertility, such as polycystic ovary syndrome endometriosis, well gestational complications, e.g., low birth weight, preterm delivery, miscarriages. This review highlights composition microbiome relation infertility-related disorders, endometriosis syndrome, provides a comprehensive overview current state knowledge on relationship dysbiotic microbiome, pregnancy, its impact reproductive tract.

Язык: Английский

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Bacteriophage-Based Therapies in Oral Cancer: A New Frontier in Oncology

Cancer Pathogenesis and Therapy, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Tongue coating microbiota-based machine learning for diagnosing digestive system tumours

Journal of Oral Microbiology, Год журнала: 2025, Номер 17(1)

Опубликована: Апрель 5, 2025

Digestive system tumours (DSTs) often diagnosed late due to nonspecific symptoms. Non-invasive biomarkers are crucial for early detection and improved outcomes. We collected tongue coating samples from 710 patients with DST 489 healthy controls (HC) April 2023, December 2023. Microbial composition was analyzed using 16S rRNA sequencing, five machine learning algorithms were applied assess the diagnostic potential of microbiota. Alpha diversity analysis showed that microbial in significantly increased patients. LEfSe identified DST-enriched genera Alloprevotella Prevotella, contrasting HC-dominant taxa Neisseria, Haemophilus, Porphyromonas (LDA >4). Notably, when comparing each four subtypes HC group, proportion Haemophilus group higher, it as an important feature distinguishing group. Machine validation demonstrated superior performance Extreme Gradient Boosting (XGBoost) model, achieving AUC 0.926 (95% CI: 0.893-0.958) internal validation, outperforming other models. Tongue microbiota shows promise a non-invasive biomarker diagnosis, supported by robust

Язык: Английский

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Optimizing Cancer Treatment Through Gut Microbiome Modulation

Cancers, Год журнала: 2025, Номер 17(7), С. 1252 - 1252

Опубликована: Апрель 7, 2025

The gut microbiome plays a pivotal role in modulating cancer therapies, including immunotherapy and chemotherapy. Emerging evidence demonstrates its influence on treatment efficacy, immune response, resistance mechanisms. Specific microbial taxa enhance checkpoint inhibitor while dysbiosis can contribute to adverse outcomes. Chemotherapy effectiveness is also influenced by composition, with engineered probiotics prebiotics offering promising strategies drug delivery reduce toxicity. Moreover, metabolites, such as short-chain fatty acids, systems have shown potential improve therapeutic responses. These findings underscore the importance of personalized microbiome-based approaches optimizing treatments.

Язык: Английский

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