Development of a duplex real-time recombinase aided amplification assay for the simultaneous and rapid detection of PCV3 and PCV4

Virology Journal, Год журнала: 2025, Номер 22(1)

Опубликована: Фев. 1, 2025

Porcine circoviruses 3 (PCV3) and 4 (PCV4) are emerging pathogens with global implications for swine industry, disturbing the diagnosis of PCVs associated diseases due to a range similar clinical symptoms increasingly coinfections. A rapid accurate method detection PCV3 PCV4 is critical controlling transmission disease. We developed duplex real-time recombinase aided amplification (RAA) assay both simultaneously. The was completed within 20 min at 39℃ designed optimal primers probes. established more convenient simpler operation compared conventional molecular biological assays. achieved limit 73.67 copies/reaction each circovirus (at 95% probability by probit regression analysis) showed high specificity no cross-reactivity other important porcine viruses (including PCV2). intra- inter-group coefficients variation (CV) were ranged from 2.08 4.97%, indicating stability reliability. Comparative analysis qPCR on 60 samples artificially spiked indicated congruence (the kappa value 0.966 1, respectively, p < 0.001), only minor discrepancies, validating effectiveness RAA in detecting co-infections its suitability preliminary PCV4. This study provides robust basis multiplex veterinary using technique, enhancing field's capacity control PCV4, supporting reliable aid epidemiological understanding circoviruses.

Язык: Английский

Molecular detection and genetic characteristics of porcine circovirus 3 and porcine circovirus 4 in central China

Archives of Virology, Год журнала: 2024, Номер 169(5)

Опубликована: Май 1, 2024

Язык: Английский