Beni-Suef University Journal of Basic and Applied Sciences,
Год журнала:
2025,
Номер
14(1)
Опубликована: Май 12, 2025
Abstract
Background
In
recent
efforts
to
address
the
critical
need
for
clean
and
portable
water,
we
have
focused
on
innovative
methods
eliminate
pathogenic
microorganisms.
To
this
aim,
N-Glycyl-L-leucine
(
Gly-Leu)
peptide
ligand
was
complexed
with
different
transition
metal
ions
[Cu(II),
Ni(II),
Cd(II)]
as
new
metalloantibiotics.
The
compounds
were
characterized
examined
using
various
analytical
methods,
including
elemental
analysis
(CHN),
Fourier
transform
infrared
spectroscopy
(FTIR),
assessments
of
magnetic
properties,
molar
conductivity,
1
HNMR,
thermogravimetric
(TGA),
mass
spectroscopy.
acted
a
di-anionic
molecule
carboxylate
deprotonated
amide
nitrogen
atom.
coordination
sites
completed
carbonyl
oxygen
atoms
water
molecule.
complexes
showed
polymeric
structures
bridging
groups.
Results
antibacterial
properties
synthesized
chelate
evaluated
disk
diffusion
minimum
inhibitory
concentration
bacterial
organisms
identified
from
samples
taken
Nile
River.
At
mg/mL
dose,
Cu(II)-chelate
biggest
zone
27
mm
against
Klebsiella
pneumonia,
MIC
value
62.5
μg/mL,
greater
than
that
common
gentamicin
medication.
Molecular
docking
investigations
supported
these
findings,
showing
had
lowest
binding
energy
−
6.16
kcal/mol,
indicating
significant,
beneficial
interactions
amino
acids
in
active
region
proteins.
Furthermore,
Cu(II)
complex
COVID-19
main
protease
encouraging
results
analysis,
may
antiviral
be
able
inhibit
viral
propagation
successfully.
chelates
demonstrated
noteworthy
antioxidant
activity,
especially
1,1-diphenyl-2-picrylhydrazyl
(DPPH
radicals).
IC
50
values
assay
Ni(II)
extremely
similar
ascorbic
acid,
antioxidant.
Their
notable
capacity
by
(14.4,
15.5,
18
µg/mL)
chelates,
respectively.
Conclusions
Our
study
successfully
potential
Gly-Leu
ions,
particularly
Cu(II),
eliminating
microorganisms
water.
exhibited
superior
confirmed
both
experimental
molecular
results.
also
displayed
capacity,
comparable
acid.
Additionally,
promising
potential,
theoretically
interacting
effectively
protease,
which
suggests
its
ability
replication.
These
underscore
multi-functional
compound
applications
purification
therapeutic
fields.
Current Research in Biotechnology,
Год журнала:
2024,
Номер
7, С. 100187 - 100187
Опубликована: Янв. 1, 2024
The
river
Ganges
in
India
has
faced
considerable
issues
due
to
water
quality
degradation
caused
by
various
anthropogenic
activities.
This
study
employs
metagenomic
analysis
comprehensively
characterize
bacterial
communities,
explore
functional
genomics,
and
investigate
the
prevalence
of
virulence
factors
antibiotic
resistant
genes
(ARGs)
within
sediment
environment
Ganges.
Taxonomic
profiling
revealed
that
proteobacteria
were
most
dominating
phyla
found
all
samples,
whereas
abundance
pseudomonas
at
genus
level
was
highest
samples.
Functional
annotation
pathway
uncover
genomic
potential
sediment-associated
bacteria,
shedding
light
on
metabolic
pathways,
biogeochemical
processes,
human
diseases
adaptive
mechanisms
riverine
ecosystem.
Moreover,
identifying
number
related
observed
K1
samples
(3),
ARGs
K3
(25)
emphasizes
need
understand
pathogenicity
these
environments.
Characterization
provides
crucial
insights
into
resistance
determinants,
their
genetic
contexts,
sources
this
vital
aquatic
Overall
metagenomics
different
sampling
sites
Ganga
nearly
same
OTUs
microbial
communities
taxonomic
but
not
a
level.
research
can
be
critical
foundation
for
assessing
ecological
implications
resistome
sediments.
findings
underscore
importance
approaches
elucidating
intricate
ecology
elements
relevant
environmental
health
antimicrobial
ecosystems.
Further
investigation
would
required
underlying
cause
behind
restoration
profiles,
including
VFs,
unravel
rejuvenation
aspects
unique
Foods,
Год журнала:
2024,
Номер
13(23), С. 3886 - 3886
Опубликована: Дек. 1, 2024
Foodborne
pathogens
are
microorganisms
that
cause
illness
through
contamination,
presenting
significant
risks
to
public
health
and
food
safety.
This
review
explores
the
metabolites
produced
by
these
pathogens,
including
toxins
secondary
metabolites,
their
implications
for
human
health,
particularly
concerning
cancer
risk.
We
examine
various
such
as
Salmonella
sp.,
Campylobacter
Escherichia
coli,
Listeria
monocytogenes,
detailing
specific
of
concern
carcinogenic
mechanisms.
study
discusses
analytical
techniques
detecting
chromatography,
spectrometry,
immunoassays,
along
with
challenges
associated
detection.
covers
effective
control
strategies,
processing
techniques,
sanitation
practices,
regulatory
measures,
emerging
technologies
in
pathogen
control.
manuscript
considers
broader
highlighting
importance
robust
policies,
awareness,
education.
identifies
research
gaps
innovative
approaches,
recommending
advancements
detection
methods,
preventive
policy
improvements
better
manage
foodborne
metabolites.
Rapid
and
precise
detection
of
waterborne
pathogens
is
critical
for
effective
public
health
management
environmental
safety.
Traditional
methods
water
quality
monitoring,
considered
the
"Gold
Standard,"
are
time-consuming,
costly,
rely
on
centralized
laboratories
expertise.
These
impractical
on-site,
real-time
requiring
further
improvements.
This
review
explores
various
cutting-edge
molecular
techniques.
It
highlights
growing
importance
point-of-care
point-of-application
to
expedite
results
improve
risk
management.
Loop-mediated
isothermal
amplification
emerges
as
a
reliable,
rapid,
accessible
tool
in
realms
on-site
diagnostics
surveillance.
Moreover,
emphasizes
crucial
role
sample
preparation
in-field
nucleic
acid
isolation
augmenting
pathogen
enable
assessments
quality.
The
advancement
these
techniques
will
guarantee
access
safe
diseases.
Diagnostics,
Год журнала:
2024,
Номер
14(15), С. 1598 - 1598
Опубликована: Июль 25, 2024
Digital
polymerase
chain
reaction
(dPCR)
has
emerged
as
a
groundbreaking
technology
in
molecular
biology
and
diagnostics,
offering
exceptional
precision
sensitivity
nucleic
acid
detection
quantification.
This
review
highlights
the
core
principles
transformative
potential
of
dPCR,
particularly
infectious
disease
diagnostics
environmental
surveillance.
Emphasizing
its
evolution
from
traditional
PCR,
dPCR
provides
accurate
absolute
quantification
target
acids
through
advanced
partitioning
techniques.
The
addresses
significant
impact
sepsis
diagnosis
management,
showcasing
superior
specificity
early
pathogen
identification
drug-resistant
genes.
Despite
advantages,
challenges
such
optimization
experimental
conditions,
standardization
data
analysis
workflows,
high
costs
are
discussed.
Furthermore,
we
compare
various
commercially
available
platforms,
detailing
their
features
applications
clinical
research
settings.
Additionally,
explores
dPCR's
role
water
microbiology,
wastewater
surveillance
monitoring
waterborne
pathogens,
underscoring
importance
public
health
protection.
In
conclusion,
future
prospects
including
methodological
optimization,
integration
with
innovative
technologies,
expansion
into
new
sectors
like
metagenomics,
explored.
RSC Advances,
Год журнала:
2024,
Номер
14(39), С. 28487 - 28515
Опубликована: Янв. 1, 2024
The
lack
of
reliable
and
efficient
techniques
for
early
monitoring
to
stop
long-term
effects
on
human
health
is
an
increasing
problem
as
the
pathogenesis
effect
infectious
bacteria
growing
continuously.
Therefore,
developing
effective
detection
technique
coupled
with
continuous
pathogenic
increasingly
becoming
a
global
public
prime
target.
Electrochemical
biosensors
are
among
strategies
that
can
be
utilized
accomplishing
goal
promising
potential.
In
recent
years,
identifying
target
biological
analytes
by
interacting
bioreceptors
modified
electrodes
most
commonly
used
in
electrochemical
biosensing
strategies.
employed
nucleic
acid
molecules
(DNA
or
RNA),
proteins,
antibodies,
enzymes,
organisms,
tissues,
biomimetic
components
such
molecularly
imprinted
polymers.
Despite
advancement
biosensing,
biosensor
detecting
still
infancy
stage
so
much
room
growth.
A
major
milestone
addressing
some
issues
improving
pathway
investigation
specific
bacterial
techniques.
present
study
covers
fundamental
concepts
biosensors,
PB
illnesses,
latest
based
bioreceptor
elements
designed
detect
bacteria.
This
aims
assist
researchers
up-to-date
research
work
field
bio-electrochemical
monitoring.
