Synthesis of new Cu(II), Ni(II), and Cd(II)-(N-Glycyl-L-leucine) complexes as peptide metalloantibiotics for targeting pathogenic water with antioxidant effect investigation DOI Creative Commons
Safaa S. Hassan,

Eman F. Mohamed,

Kirolos Maged

и другие.

Beni-Suef University Journal of Basic and Applied Sciences, Год журнала: 2025, Номер 14(1)

Опубликована: Май 12, 2025

Abstract Background In recent efforts to address the critical need for clean and portable water, we have focused on innovative methods eliminate pathogenic microorganisms. To this aim, N-Glycyl-L-leucine ( Gly-Leu) peptide ligand was complexed with different transition metal ions [Cu(II), Ni(II), Cd(II)] as new metalloantibiotics. The compounds were characterized examined using various analytical methods, including elemental analysis (CHN), Fourier transform infrared spectroscopy (FTIR), assessments of magnetic properties, molar conductivity, 1 HNMR, thermogravimetric (TGA), mass spectroscopy. acted a di-anionic molecule carboxylate deprotonated amide nitrogen atom. coordination sites completed carbonyl oxygen atoms water molecule. complexes showed polymeric structures bridging groups. Results antibacterial properties synthesized chelate evaluated disk diffusion minimum inhibitory concentration bacterial organisms identified from samples taken Nile River. At mg/mL dose, Cu(II)-chelate biggest zone 27 mm against Klebsiella pneumonia, MIC value 62.5 μg/mL, greater than that common gentamicin medication. Molecular docking investigations supported these findings, showing had lowest binding energy − 6.16 kcal/mol, indicating significant, beneficial interactions amino acids in active region proteins. Furthermore, Cu(II) complex COVID-19 main protease encouraging results analysis, may antiviral be able inhibit viral propagation successfully. chelates demonstrated noteworthy antioxidant activity, especially 1,1-diphenyl-2-picrylhydrazyl (DPPH radicals). IC 50 values assay Ni(II) extremely similar ascorbic acid, antioxidant. Their notable capacity by (14.4, 15.5, 18 µg/mL) chelates, respectively. Conclusions Our study successfully potential Gly-Leu ions, particularly Cu(II), eliminating microorganisms water. exhibited superior confirmed both experimental molecular results. also displayed capacity, comparable acid. Additionally, promising potential, theoretically interacting effectively protease, which suggests its ability replication. These underscore multi-functional compound applications purification therapeutic fields.

Язык: Английский

High Sensitivity Terahertz Biosensor Based on Graphene/Methylammonium Lead Halide Metasurface with Machine Learning-Enhanced Pathogen Detection DOI
Jacob Wekalao, Niranjana Siddharthan,

Soman Shibu

и другие.

Plasmonics, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 25, 2024

Язык: Английский

Процитировано

19

Metagenomics analysis of sediments of river Ganga, India for bacterial diversity, functional genomics, antibiotic resistant genes and virulence factors DOI Creative Commons
Ajaya Kumar Rout, Partha Sarathi Tripathy, Sangita Dixit

и другие.

Current Research in Biotechnology, Год журнала: 2024, Номер 7, С. 100187 - 100187

Опубликована: Янв. 1, 2024

The river Ganges in India has faced considerable issues due to water quality degradation caused by various anthropogenic activities. This study employs metagenomic analysis comprehensively characterize bacterial communities, explore functional genomics, and investigate the prevalence of virulence factors antibiotic resistant genes (ARGs) within sediment environment Ganges. Taxonomic profiling revealed that proteobacteria were most dominating phyla found all samples, whereas abundance pseudomonas at genus level was highest samples. Functional annotation pathway uncover genomic potential sediment-associated bacteria, shedding light on metabolic pathways, biogeochemical processes, human diseases adaptive mechanisms riverine ecosystem. Moreover, identifying number related observed K1 samples (3), ARGs K3 (25) emphasizes need understand pathogenicity these environments. Characterization provides crucial insights into resistance determinants, their genetic contexts, sources this vital aquatic Overall metagenomics different sampling sites Ganga nearly same OTUs microbial communities taxonomic but not a level. research can be critical foundation for assessing ecological implications resistome sediments. findings underscore importance approaches elucidating intricate ecology elements relevant environmental health antimicrobial ecosystems. Further investigation would required underlying cause behind restoration profiles, including VFs, unravel rejuvenation aspects unique

Язык: Английский

Процитировано

14

Impact of Metabolites from Foodborne Pathogens on Cancer DOI Creative Commons
Alice Njolke Mafe, Dietrich Büsselberg

Foods, Год журнала: 2024, Номер 13(23), С. 3886 - 3886

Опубликована: Дек. 1, 2024

Foodborne pathogens are microorganisms that cause illness through contamination, presenting significant risks to public health and food safety. This review explores the metabolites produced by these pathogens, including toxins secondary metabolites, their implications for human health, particularly concerning cancer risk. We examine various such as Salmonella sp., Campylobacter Escherichia coli, Listeria monocytogenes, detailing specific of concern carcinogenic mechanisms. study discusses analytical techniques detecting chromatography, spectrometry, immunoassays, along with challenges associated detection. covers effective control strategies, processing techniques, sanitation practices, regulatory measures, emerging technologies in pathogen control. manuscript considers broader highlighting importance robust policies, awareness, education. identifies research gaps innovative approaches, recommending advancements detection methods, preventive policy improvements better manage foodborne metabolites.

Язык: Английский

Процитировано

12

Advances in point-of-care and molecular techniques to detect waterborne pathogens DOI Creative Commons
Meysam Khodaparast, David Sharley, Stephen W. Marshall

и другие.

npj Clean Water, Год журнала: 2024, Номер 7(1)

Опубликована: Авг. 12, 2024

Rapid and precise detection of waterborne pathogens is critical for effective public health management environmental safety. Traditional methods water quality monitoring, considered the "Gold Standard," are time-consuming, costly, rely on centralized laboratories expertise. These impractical on-site, real-time requiring further improvements. This review explores various cutting-edge molecular techniques. It highlights growing importance point-of-care point-of-application to expedite results improve risk management. Loop-mediated isothermal amplification emerges as a reliable, rapid, accessible tool in realms on-site diagnostics surveillance. Moreover, emphasizes crucial role sample preparation in-field nucleic acid isolation augmenting pathogen enable assessments quality. The advancement these techniques will guarantee access safe diseases.

Язык: Английский

Процитировано

10

Paper-based biosensing using single-stranded oligonucleotide aptamers for enhanced food safety DOI Creative Commons

Kiyana Fatemi,

Sie Yon Lau,

Reza Fatemi

и другие.

Journal of Food Composition and Analysis, Год журнала: 2025, Номер unknown, С. 107331 - 107331

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

2

Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings DOI Creative Commons

Alessia Mirabile,

Giuseppe Sangiorgio, Paolo Giuseppe Bonacci

и другие.

Diagnostics, Год журнала: 2024, Номер 14(15), С. 1598 - 1598

Опубликована: Июль 25, 2024

Digital polymerase chain reaction (dPCR) has emerged as a groundbreaking technology in molecular biology and diagnostics, offering exceptional precision sensitivity nucleic acid detection quantification. This review highlights the core principles transformative potential of dPCR, particularly infectious disease diagnostics environmental surveillance. Emphasizing its evolution from traditional PCR, dPCR provides accurate absolute quantification target acids through advanced partitioning techniques. The addresses significant impact sepsis diagnosis management, showcasing superior specificity early pathogen identification drug-resistant genes. Despite advantages, challenges such optimization experimental conditions, standardization data analysis workflows, high costs are discussed. Furthermore, we compare various commercially available platforms, detailing their features applications clinical research settings. Additionally, explores dPCR's role water microbiology, wastewater surveillance monitoring waterborne pathogens, underscoring importance public health protection. In conclusion, future prospects including methodological optimization, integration with innovative technologies, expansion into new sectors like metagenomics, explored.

Язык: Английский

Процитировано

9

A microfluidic system for rapid enrichment and sensitive detection of E. coli based on bilayer membrane and high flux droplets DOI

Pengfei Gong,

Zehang Gao, Rui Sun

и другие.

Talanta, Год журнала: 2025, Номер unknown, С. 128005 - 128005

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

1

Current microbiological challenges in drinking water DOI Creative Commons
Ana Afonso, María José Saavedra, Inês B. Gomes

и другие.

Journal of Water Process Engineering, Год журнала: 2025, Номер 72, С. 107614 - 107614

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

1

Electrochemical disinfection for human health protection:Disinfection Mechanisms, System Innovations, Applications DOI
Xinyuan Li, Zhen Li,

Duowen Yang

и другие.

Journal of environmental chemical engineering, Год журнала: 2024, Номер 12(5), С. 114073 - 114073

Опубликована: Сен. 6, 2024

Язык: Английский

Процитировано

6

Bioreceptor modified electrochemical biosensors for the detection of life threating pathogenic bacteria: a review DOI Creative Commons
Abdul Khaleque, Syed Imdadul Hossain, Md. Romzan Ali

и другие.

RSC Advances, Год журнала: 2024, Номер 14(39), С. 28487 - 28515

Опубликована: Янв. 1, 2024

The lack of reliable and efficient techniques for early monitoring to stop long-term effects on human health is an increasing problem as the pathogenesis effect infectious bacteria growing continuously. Therefore, developing effective detection technique coupled with continuous pathogenic increasingly becoming a global public prime target. Electrochemical biosensors are among strategies that can be utilized accomplishing goal promising potential. In recent years, identifying target biological analytes by interacting bioreceptors modified electrodes most commonly used in electrochemical biosensing strategies. employed nucleic acid molecules (DNA or RNA), proteins, antibodies, enzymes, organisms, tissues, biomimetic components such molecularly imprinted polymers. Despite advancement biosensing, biosensor detecting still infancy stage so much room growth. A major milestone addressing some issues improving pathway investigation specific bacterial techniques. present study covers fundamental concepts biosensors, PB illnesses, latest based bioreceptor elements designed detect bacteria. This aims assist researchers up-to-date research work field bio-electrochemical monitoring.

Язык: Английский

Процитировано

5