Molecular Characterization and Plant Growth Promotion Potential of Paenibacillus Dendritiformis Endophyte Isolated from Tecomella Undulata (Roheda)

Current Agriculture Research Journal, Год журнала: 2025, Номер 12(3), С. 1170 - 1180

Опубликована: Янв. 15, 2025

In this study, we have isolated a bacterial endophyte Paenibacillus dendritiformis strain RAE13 (Accession number: OR259131) from the leaves of Tecomella undulata (Roheda) plant. The identification species was carried out using 16s-rDNA ribotyping. Subsequently, gauged for its potential to endorse plant growth through various mechanisms such as nitrogen fixation, IAA production, HCN synthesis, siderophore generation, and ammonia production. Furthermore, evaluation focused on endophyte's capacity producing extracellular enzymes, including cellulase, chitinase, protease, amylase, catalase. exhibited synthesize an average 18±0.375 μg/ml indole-3-acetic acid (IAA) after being subjected concentration 5 mg/ml tryptophan over 14-day incubation period. endophytic isolate RAE 13 produced 42.4±0.004 Gibberellin, solubilized phosphate in range 70.2 135.5 μg/ml, 45.5 ammonia. phylogenetic analysis unveiled that had common ancestor maximum nucleotide sequence similarity 98.30% with sp isolates Uttar Pradesh, India. To diminish consumption chemicals conventional farming, results indicated great growth-stimulating inoculant. Henceforward, utilization these enzymes medical industrial applications will be highly beneficial. Additionally, it could enhance tolerance challenging environmental circumstances drought high temperatures.

Язык: Английский

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Unveiling the roles, mechanisms and prospects of soil microbial communities in sustainable agriculture

Discover Soil., Год журнала: 2025, Номер 2(1)

Опубликована: Фев. 17, 2025

Abstract Soil hosts diverse microbial communities including bacteria, fungi, archaea, protozoans and nematodes among others, which are fundamental to sustainable agriculture drive essential processes that underpin soil fertility, plant health, ecosystem resilience. They promote growth through mechanisms like nitrogen fixation, phosphorus solubilization, production of growth-promoting substances, enhancement nutrient uptake, improvement structure detoxification harmful substances. Recently, there has been increasing interest in utilizing microorganisms improve health boost efficiency, despite limited understanding diversity, microbe-plant interactions, translating laboratory findings field conditions. This is driven by the urgent need feed growing global population, placing pressure on arable land produce high-quality yields. Conventionally, synthetic fertilizers have extensively used provide nutrients, growth, increase crop productivity. Although revolutionized since green revolution, their overuse significantly harmed reduced review synthesizes current knowledge microbes influence agricultural practices, with a focus cycling plant–microbe interactions. We discuss functions important groups, such as nitrogen-fixing phosphorus-solubilizing bacteria (PSB), fungi. Furthermore, we factors highlight gaps future research maximize potential use agriculture. Understanding significance key increases harnessing vital contributors

Язык: Английский

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Groundnut (Arachis hypogaea L.) growth and yield enhancement by consortium of diazotrophic and non-rhizobial root nodule endophytic bacteria

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

Modern agriculture rely heavily on costly and environmentally pernicious chemical fertilizers. To investigate possible alternatives to fertilization in A. hypogaea cultivation, consortium of three plant growth promoting bacteria (PGPB): P. aeruginosa, A. chrococcum and faecalis were isolated from rhizosphere root nodules using spread plate dilution method Azotobacter chroococcum agar, yeast extract manitol agar media. Isolates identified using cultural, morphological, biochemical molecular characterization. were scaled-up, inoculated into 4000.0g soil pots at rate 4.5 x 10⁶ cfu/ml (A. ), 2.0 10⁹ (P. aeruginosa), 1.5 10⁹ cfu/ml faecalis) the following treatments: Uninoculated (control); NPK; Pseudomonas-Azotobacter; Pseudomonas-Alcaligenes; Pseudomonas-Alcaligenes-Azotobacter in triplicates. Seeds hypogaea belonging two varieties (SAMNUT 25 26) propagated treated soils separate sets. Growth enhancement parameters: germination time, percentage germination, shoot, lengths growth, fresh pods weights, nodule numbers, properties determined after 120 days. Results showed with PGPB enhanced all parameters over uninoculated (control) NPK which statistically significant for lengths, numbers (p < 0.5). Soil properties: pH, P, N, CEC SOM contents maintained enhanced. and soil inoculation offered effective alternative fertilization.

Язык: Английский

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Role of Carbon-Based Nanomaterials in Crop Plants Temperature/Heat Stress Management

Опубликована: Янв. 1, 2025

Язык: Английский

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Impact of Carbon-Based Nanomaterials on Soil Microbiomes and Associated Activities

Опубликована: Янв. 1, 2025

Язык: Английский

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Enhancing nickel stress tolerance in Micro-Tom tomatoes through biopriming with Paraburkholderia phytofirmans PsJN: insights into growth and physiological responses

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 13, 2025

Introduction The strategic utilization of plant growth-promoting (PGP) rhizospheric bacteria is a sustainable approach to mitigating the negative effects anthropogenic activities and excessive nickel (Ni) accumulation in plants. Given that specific symbiotic interactions depend on direct relationship between species, bacterial strain, heavy metals (HMs), this study aimed investigate Paraburkholderia phytofirmans PsJN seed priming Ni tolerance adult Micro-Tom tomato plants ( Solanum lycopersicum L .). Methods Sterilized seeds were bioprimed with P. for 24 hours then sown into soil. Non-primed, imbibed used as control. After 10 days, seedlings transferred Hoagland nutrient solution. Chronic (10 μM Ni) acute (50 stress conditions induced by supplementing solution salt. experiment lasted approximately 75 covering complete life cycle Various physiological biochemical parameters analyzed. Results Significant differences p &lt; 0.05) observed non-primed terms fruit yield. Bioprimed tomatoes exhibited higher resilience stress, particularly under conditions. Non-primed treated 50 showed statistically lower concentrations chlorophyll total chlorophylls compared tomatoes. Moreover, proline content was generally more stable plants, indicating reduced oxidative stress.The activity antioxidant enzymes distinct patterns nonprimed Conclusion findings suggest biopriming enhances growth stress. This technique appears mitigate Ni-induced effects, at concentrations, making it promising strategy improving performance Ni-contaminated environments. Future studies should explore underlying molecular mechanisms field applications approach.

Язык: Английский

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Biopriming of Pseudomonas aeruginosa Abates Fluoride Toxicity in Oryza sativa L. by Restricting Fluoride Accumulation, Enhancing Antioxidative System, and Boosting Activities of Rhizospheric Enzymes

Plants, Год журнала: 2025, Номер 14(8), С. 1223 - 1223

Опубликована: Апрель 16, 2025

Plant growth-promoting bacteria (PGPB) are free-living microorganisms that actively reside in the rhizosphere and affect plant growth development. These employ their own metabolic system to fix nitrogen, solubilize phosphate, secrete hormones directly impact metabolism of plants. Generating sustainable agricultural production under various environmental stresses requires a detailed understanding mechanisms use promote growth. In present study, Pseudomonas aeruginosa (MW843625), PGP soil bacterium with minimum inhibitory concentration (MIC) 150 mM against fluoride (F), was isolated from fields Chhattisgarh, India, assessed for remedial potential. This study concentrated on biomass accumulation, nutrient absorption, oxidative stress tolerance plants involving antioxidative enzymes. By determining MDA accumulation ROS (O2•− H2O2) Oryza sativa L. F (50 ppm) stress, assessed. The results showed inoculation P. enhanced ability O. seedlings absorb nutrients increased amounts total chlorophyll (Chl), soluble protein, biomass. contrast cultivated F-stress alone, those inoculated along considerably reduced concentrations roots, shoots, grains. alleviation deleterious effects owing has been associated improved activity/upregulation genes (SOD, CAT, APX) comparison only F-subjected plants, which resulted lower O2•−, H2O2, content. Additionally, it also reflected our potential increase activities enzymes such as urease, phosphatase, dehydrogenase, nitrate reductase, cellulase. Accordingly, findings conducted suggest can be exploited not an ideal candidate bioremediation but enhancing fertility promotion development contamination.

Язык: Английский

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Multidimensional role of selenium nanoparticles to promote growth and resilience dynamics of Phaseolus vulgaris against sodium fluoride stress

International Journal of Phytoremediation, Год журнала: 2024, Номер unknown, С. 1 - 18

Опубликована: Дек. 16, 2024

High fluoride (F) concentrations negatively affect the seed germination, plant growth, development, and yield of crops.

Язык: Английский

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Molecular Characterization of Plant Growth Promoting Rhizobacteria Isolated from Nodules of Cicer arietinum Plant

Current Agriculture Research Journal, Год журнала: 2024, Номер 12(2), С. 705 - 713

Опубликована: Сен. 10, 2024

Agriculture has recently prioritized Plant Growth Promoting Rhizobacteria (PGPR) due to their significance in the rhizosphere, which is an ecological unit biosphere. Isolating PGPR from Cicer arietinum (chickpea) nodules and characterizing its PGP activities was purpose of present study. The research showed that specific strain could manufacture several useful chemicals, such as hydrogen cyanide (HCN), ammonia, siderophore, indole acetic acid (IAA) nitrogenase. Furthermore, isolate identified Bacillus licheniformis AS11 through 16s rRNA analysis, alignment analysis 99% similarity with KPA12 isolate. It's worth highlighting that, a potential PGPR, considered valuable asset for agriculture when it positive effect on plant growth. Therefore, can be beneficial constructive addition field agriculture. These findings suggest applications sustainable by improving crop yield natural growth-promoting mechanisms reducing reliance chemical fertilizers.

Язык: Английский

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Mechanisms and Use of Plant Growth-Promoting Bacteria to Improve Seed Germination in Adverse Environments

IntechOpen eBooks, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 5, 2024

Seed dormancy and germination are highly regulated processes under the control of various factors, among which stand out internal balance abscisic acid (ABA)/gibberellin (GA) environmental conditions that impact this ratio. Germination determines when plants enter to natural or agricultural ecosystems. It represents basis production, several strategies have been implemented improve it. Plant Growth Promoting Rhizobacteria (PGPR) ubiquitous soil microorganisms favorably plant performance through pathogen control, nutrient uptake, tolerance stress conditions, even production growth regulators, both seed subsequent development. Seeds germinate as soon they embedded sown, so most studies focused on mechanisms depend themselves, it is evident a better knowledge plant-microorganism interactions could be useful achieve sustainable ecologically friendly agriculture. Here, after describing endogenous controlling dormancy, we will update information about potential PGPR germination, give general overview their use in agriculture systems guarantee food security years come.

Язык: Английский

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