Molecular Characterization of Whole Genome Sequencing of Salmonella spp. in Shapingba District, Chongqing, China, 2016–2023

Foodborne Pathogens and Disease, Год журнала: 2025, Номер unknown

Опубликована: Янв. 21, 2025

In recent years, Salmonella infection is a major global public health concern, particularly in food safety. This study analyzed the genomes of 102 strains isolated between 2016 and 2023 from food, foodborne disease patients, poisoning incidents, focusing on their molecular characteristics, antibiotic resistance genes (ARGs), virulence genes. S. enterica serovar Enteritidis (37.3%) Typhimurium (21.6%, including its monophasic variant 1,4,[5],12:i:-) were main among 22 serotypes. Multilocus sequence typing revealed 23 types (STs), with ST11, ST19, ST34 as most prevalent. All carried at least 24 ARGs. Detection rates for aac(6')-Iy, blaTEM-1, sul2 ranged 44.1% to 63.7%, mainly Typhimurium. Rates qnrS1, sul1, aadA 12.8% 16.7%, while mcr-1 appeared one strain. contained 98 The pefABCD, mig-5, spvBCD 48.0% strains, rck was found 36.3%, linked tssM gene 37.3% exclusively Enteritidis. Core genome single nucleotide polymorphisms (cgSNPs) analysis grouped into nine clusters, 75.5% belonging three groups. Food event 1 correlated cluster 3, events 2 3 1. Across events, SNP differences ≤6. Strains ≤10 also other clusters. method promising tracking sporadic cases identifying potential safety incidents. region exhibit substantial genetic diversity, demonstrating efficacy cgSNPs source tracking. Ongoing surveillance essential given prevalence ARGs provides data foundation local epidemiology.

Язык: Английский

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Epidemiological Features of Nontyphoidal Salmonella Infections Reported to Foodborne Disease Surveillance System in China, 2013–2022

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

Background Nontyphoidal Salmonella (NTS) is the most commonly reported foodborne gastrointestinal infection and ranks among top three causes of bacterial outbreaks in China. Methods This study analyzed NTS data from Foodborne Disease Surveillance System China (2013–2022) to summarize epidemiological trends, assess reporting rate changes, examine rural-urban variations. Results During this period, 55,266 cases were reported, with rates increasing significantly (AAPC [CRR] = 21.89%). Urban areas accounted for 70.06% cases, but rural patients had higher hospitalization than urban ones (41.54% vs. 33.69%, p < 0.001). Children aged 0–4 years constituted 53.59% showed (p 0.05). Typhimurium (31.07%) Enteritidis (15.89%) leading serotypes. strongly correlated national per-capita consumption fruit (r 0.98), poultry 0.95), aquatic products eggs 0.94), vegetables 0.79), dairy 0.73), meat 0.65). Conclusion highlights growing public health burden China, a sharp rise major differences. Over half involved children under 5 old, underscoring urgent need enhance food safety measures, particularly vulnerable populations.

Язык: Английский

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Dynamic Serotype Distribution and Antimicrobial Resistance of Salmonella Isolates from 2019 to 2023 in Guizhou, China

Infection and Drug Resistance, Год журнала: 2025, Номер Volume 18, С. 993 - 1006

Опубликована: Фев. 1, 2025

Salmonella, a leading cause of human infectious diarrhea diseases, foodborne illness, and zoonotic infections, poses significant health burden. A retrospective screening was performed to elucidate the serotype distribution antimicrobial resistance 933 Salmonella isolates from nine cities (prefectures) in Guizhou province southwestern China between 2019 2023 through slide agglutination testing. Fifty-four different serotypes were identified this study, with S. Typhimurium (44.4%) Enteritidis (20.7%) being predominant serotypes, followed by London (3.1%), Derby (2.8%), Rissen (2.0%). total 39 reported for first time province, 121 (13.0%) could not be classified. The diversity has increased 8 2023. In addition, detection rate showed decreasing trend over time, while demonstrated an annual increase since 2020. For isolates, majority (94.0%) exhibited at least one class agents. highest observed ampicillin (86.4%), tetracycline (76.3%) streptomycin (72.8%). Notably, we discovered that colistin 4.7%, 93.2% these Enteritidis. Meanwhile, 78.5% multidrug (MDR), MDR rates exceeding 90%. Additionally, 5.7% extensively drug-resistant (XDR), exhibiting XDR 5.1% 4.1%, respectively. peaked then gradually declined 2020 2022, rising again Our research revealed increasing within alongside challenges posed rate. Therefore, it is essential continuously improve surveillance keep track changes dynamic shifts, strengthen persistent monitoring

Язык: Английский

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Comparative genomic and antimicrobial resistance profiles of Salmonella strains isolated from pork and human sources in Sichuan, China

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 3, 2025

Introduction Salmonella detection in retail pork is increasing, yet studies on its antimicrobial resistance (AMR) profiles and genomic characteristics remain limited. Moreover, it still unclear whether certain sequence types (STs) are consistently or rarely associated with as a transmission source. Sichuan province, the largest pork-production region China, provides critical setting to investigate these dynamics. Methods In this study, 213 strains isolated from human sources (2019–2021) underwent phenotypic AMR testing whole-genome sequencing (WGS). Results Resistance profiling revealed higher prevalence of pork-derived strains, particularly veterinary-associated antibiotics. We identified STs not observed such ST23 ( S . Oranienburg) poultry-commonly ST32 Infantis), suggesting potential non-pork routes for STs. To quantify type diversity within each sample source, we introduced index (ST = number different STs/ total isolates). The ST was 32% (49/153) human-derived isolates 20% (12/60) isolates. PERMANOVA analysis significant differences structural composition between human- p 0.001), indicating that may harbor specific more frequently. Discussion These findings highlight role reservoir STs, while also implying pathways. represents novel metric assessing across sources, offering better understanding genetic variation

Язык: Английский

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Genomic analyses reveal presence of extensively drug-resistant Salmonella enterica serovars isolated from clinical samples in Guizhou province, China, 2019–2023

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 27, 2025

The emergence of extensively drug-resistant (XDR) Salmonella in humans poses a significant public health and therapeutic challenge. However, limited data are available on XDR isolates from Guizhou province, China. This study aimed to investigate the molecular epidemiology resistance patterns clinical samples this region. A total 931 were screened for through antimicrobial susceptibility testing. These subjected whole-genome sequencing (WGS) bioinformatic analysis further systematically investigating isolates. Between 2019 2023, collected Guizhou. Of these isolates, 51 (5.5%) identified as classified into 16 serovars. Among serovars, 15 corresponded specific sequence type, except S. Typhimurium predominant 1,4,[5],12:i:-, Enteritidis, Kentucky, divided ST34, ST11, ST198, respectively. Genomic showed that all harbored at least eight genes (ARGs) multidrug efflux pumps. Highly prevalent point mutations gyrA (D87 S83) parC (S80I) detected, along with plasmid-mediated quinolone (PMQR) genes. qnrS1 gene was most common (43.1%), followed by oqxA, aac-(6')-lb-cr variant, qnrB4, qnrS2, qnrA1, qepA2, oqxB. β-lactamase blaTEM-1 (54.9%), blaCTX-M-55 (35.3%) extended-spectrum subtype. Notably, blaNDM-1 first time Guizhou, one 1,4,[5],12:i:- isolate contained mcr-1.1 gene. ARGs profiles varied carrying highest number. Ten plasmid types identified, predominantly IncHI2/IncHI2A (47.5%). Key such tetA, PMQR, blaCTX-M , mcr-1.1, located plasmids. 75.0% conjugative plasmids belonged IncHI2/IncHI2A, indicating horizontal transfer conjugation facilitates dissemination. Core genome multilocus typing (cgMLST) revealed genetic diversity, 39 core (cgSTs) no evidence outbreaks. rising prevalence province is concerning. Initial provide critical insights understanding controlling infections, aiding officials identifying emerging threats trends.

Язык: Английский

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Prevalence, antibiotic resistance, virulence and antimicrobial resistance gene profiles of Salmonella species recovered from retail beef and poultry processing environments

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 28, 2025

This study investigates the prevalence, antimicrobial resistance, genotypic resistance profiles, and virulence gene distribution of Salmonella isolates from poultry beef processing environments in Shahrekord, Iran. A total 680 samples were collected (n = 300) 380) products between January December 2023. was detected 21% (63/300) 15.8% (60/380) samples, with non-typhoidal (NTS) being predominant serovar. High (AMR) rates observed across both food types, most common resistances found ciprofloxacin (48%), tetracycline (44%), ampicillin (39%). Genotypic analysis revealed presence key genes, including blaTEM (35%), tetA (29%), sul1 (23%). Virulence identified invA (92%), agfA (80%), hilA (76%) as prevalent genes. Comparative patterns higher to tetracycline. highlights significant factors retail poultry, suggesting a potential risk public health need for enhanced surveillance control measures environments. Not applicable.

Язык: Английский

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Drug resistance and pathogenicity characteristics of Escherichia coli causing pneumonia in farmed foxes

Frontiers in Veterinary Science, Год журнала: 2025, Номер 12

Опубликована: Апрель 9, 2025

Bacterial pneumonia is a leading cause of mortality in fur-bearing animals, posing significant threat to fur production. To clarify the pathogenic agent bacterial farmed foxes from eastern Hebei province, China, we performed isolation and identification samples between 2020 2023. A total 142 strains were isolated, which 101 identified as Escherichia coli (E. coli), indicating that E. major responsible for foxes. Serotyping showed 8 serotypes prevalent isolates, with O1, O8, O78 O12 being dominant ones. Five isolates randomly picked pathogenicity testing, all them able symptoms 6-week-old Kunming mice, accompanied by organ damage lung. Eleven virulence genes demonstrated present among isolates. Antibiotic susceptibility tests 78 exhibited multi-drug resistance (MDR), highest rates against tetracyclines, some carbapenems. Notably, no single antibiotic was effective strains. Twenty (ARGs) detected Multilocus sequence typing (MLST) revealed 11 types (STs) 19 ST-101 predominating (4/19). These findings enhance our understanding epidemiology, traits, fox-derived Hebei.

Язык: Английский

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Molecular epidemiological investigation of Salmonella isolated from the environment, animals, foods and patients in China

Food Research International, Год журнала: 2024, Номер 196, С. 115013 - 115013

Опубликована: Сен. 7, 2024

Язык: Английский

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