MicroRNAs Regulate the Expression of Genes Related to the Innate Immune and Inflammatory Response in Rabbits Infected with Lagovirus europaeus GI.1 and GI.2 Genotypes DOI Open Access

Ewa Ostrycharz-Jasek,

A. Fitzner, Aldona Siennicka

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9531 - 9531

Опубликована: Сен. 2, 2024

MicroRNAs (miR) are a group of small, non-coding RNAs 17-25 nucleotides that regulate gene expression at the post-transcriptional level. Dysregulation miRNA or function may contribute to abnormal and signaling pathways, leading disease pathology. Lagovirus europaeus (L. europaeus) causes severe in rabbits called rabbit hemorrhagic (RHD). The symptoms liver, lung, kidney, spleen degeneration observed during RHD similar those acute liver failure (ALF) multi-organ (MOF) humans. In this study, we assessed miRs their target genes involved innate immune inflammatory response. Also, potential impact on pathways L. infection-two genotypes (GI.1 GI.2)-in lungs, kidneys, spleen. was determined using quantitative real-time PCR (qPCR). We miR-155 (MyD88, TAB2, p65, NLRP3), miR-146a (IRAK1, TRAF6), miR-223 (TLR4, IKKα, miR-125b (MyD88). also examined biomarkers inflammation: IL-1β, IL-6, TNF-α, IL-18 four tissues mRNA Our study shows main regulators response europaeus/GI.1 GI.2 infection, as well RHD, miR-155, miR-223, miR-146a. During infection with europaeus/RHD, has both pro- anti-inflammatory effects kidneys spleen; lungs kidneys; all tissues; however, only liver. each case, such an effect be determinant pathogenesis RHD. research three infection. However, result regulation influenced by tissue microenvironment. overexpression two critical phase cytokines: IL-6 TNF-α (in spleen). IL-1β highly expressed after These facts indicate strong rapid involvement local GI.2)-and Profile inflammation infected regarding nature changes but different for individual tissues. Therefore, propose profiles GI.1 (pulmonary, renal,

Язык: Английский

MicroRNAs Regulate the Expression of Genes Related to the Innate Immune and Inflammatory Response in Rabbits Infected with Lagovirus europaeus GI.1 and GI.2 Genotypes DOI Open Access

Ewa Ostrycharz-Jasek,

A. Fitzner, Aldona Siennicka

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9531 - 9531

Опубликована: Сен. 2, 2024

MicroRNAs (miR) are a group of small, non-coding RNAs 17-25 nucleotides that regulate gene expression at the post-transcriptional level. Dysregulation miRNA or function may contribute to abnormal and signaling pathways, leading disease pathology. Lagovirus europaeus (L. europaeus) causes severe in rabbits called rabbit hemorrhagic (RHD). The symptoms liver, lung, kidney, spleen degeneration observed during RHD similar those acute liver failure (ALF) multi-organ (MOF) humans. In this study, we assessed miRs their target genes involved innate immune inflammatory response. Also, potential impact on pathways L. infection-two genotypes (GI.1 GI.2)-in lungs, kidneys, spleen. was determined using quantitative real-time PCR (qPCR). We miR-155 (MyD88, TAB2, p65, NLRP3), miR-146a (IRAK1, TRAF6), miR-223 (TLR4, IKKα, miR-125b (MyD88). also examined biomarkers inflammation: IL-1β, IL-6, TNF-α, IL-18 four tissues mRNA Our study shows main regulators response europaeus/GI.1 GI.2 infection, as well RHD, miR-155, miR-223, miR-146a. During infection with europaeus/RHD, has both pro- anti-inflammatory effects kidneys spleen; lungs kidneys; all tissues; however, only liver. each case, such an effect be determinant pathogenesis RHD. research three infection. However, result regulation influenced by tissue microenvironment. overexpression two critical phase cytokines: IL-6 TNF-α (in spleen). IL-1β highly expressed after These facts indicate strong rapid involvement local GI.2)-and Profile inflammation infected regarding nature changes but different for individual tissues. Therefore, propose profiles GI.1 (pulmonary, renal,

Язык: Английский

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