Spatio‐temporal characteristics of the gastrointestinal resistome in a cow‐to‐calf model and its environmental dissemination in a dairy production system DOI Creative Commons
Shuai Liu, Y Zhuang, Tianyu Chen

и другие.

iMeta, Год журнала: 2025, Номер unknown

Опубликована: Май 14, 2025

Abstract Microbiome and resistome transmission from mother to child, as well animal environment, has been widely discussed in recent years. Dairy cows mainly provide milk meat. However, the dairy production system, characteristics trends of assembly microbiome gastrointestinal tract (GIT) remain unclear. In this study, we sequenced GIT (rumen fluid feces) cow populations two provinces China (136 36 calves), determined their profiles distribution antibiotics resistance genes (ARGs) across bacteria further tracked temporal dynamics offspring during early life using multi‐omics technologies (16S ribosomal RNA [rRNA] sequencing, metagenome, metatranscriptome). We characterized cows, distinguished by gut sites regions. The abundance ARGs calves peaked within first 3 days after birth, with Enterobacteriaceae dominant microbial host. As aged, composition stabilized, overall ARG gradually decreased. Both diet age influenced carbohydrate‐active enzymes profiles. Resistance ecological niches (meconium, colostrum, soil, wastewater) were unique, resembling maternal sources. Mobile genetic elements (MGEs), found soil wastewater, played an important role mediating these interactions. Multidrug consistently emerged most significant form at both metagenome metatranscriptome levels. Several antibiotic classes showed higher proportions level than DNA level, indicating that even low‐abundance gene groups can have a considerable influence through high expression. This study broadens our understanding dissemination livestock systems, providing foundation for developing future preventive control strategies.

Язык: Английский

Integrating the milk microbiome signatures in mastitis: milk-omics and functional implications DOI Creative Commons
Rine Christopher Reuben, Carmén Torres

World Journal of Microbiology and Biotechnology, Год журнала: 2025, Номер 41(2)

Опубликована: Янв. 18, 2025

Mammalian milk contains a variety of complex bioactive and nutritional components microorganisms. These microorganisms have diverse compositions functional roles that impact host health disease pathophysiology, especially mastitis. The advent use high throughput omics technologies, including metagenomics, metatranscriptomics, metaproteomics, metametabolomics, as well culturomics in microbiome studies suggest strong relationships between phenotype signatures While single undoubtedly contributed to our current understanding mastitis, they often provide limited information, targeting only biological viewpoint which is insufficient system-wide information necessary for elucidating the footprints molecular mechanisms driving mastitis dysbiosis. Therefore, integrating multi-omics approach research could generate new knowledge, improve structural ecosystem, insights sustainable control management.

Язык: Английский

Процитировано

1
Spatio‐temporal characteristics of the gastrointestinal resistome in a cow‐to‐calf model and its environmental dissemination in a dairy production system DOI Creative Commons
Shuai Liu, Y Zhuang, Tianyu Chen

и другие.

iMeta, Год журнала: 2025, Номер unknown

Опубликована: Май 14, 2025

Abstract Microbiome and resistome transmission from mother to child, as well animal environment, has been widely discussed in recent years. Dairy cows mainly provide milk meat. However, the dairy production system, characteristics trends of assembly microbiome gastrointestinal tract (GIT) remain unclear. In this study, we sequenced GIT (rumen fluid feces) cow populations two provinces China (136 36 calves), determined their profiles distribution antibiotics resistance genes (ARGs) across bacteria further tracked temporal dynamics offspring during early life using multi‐omics technologies (16S ribosomal RNA [rRNA] sequencing, metagenome, metatranscriptome). We characterized cows, distinguished by gut sites regions. The abundance ARGs calves peaked within first 3 days after birth, with Enterobacteriaceae dominant microbial host. As aged, composition stabilized, overall ARG gradually decreased. Both diet age influenced carbohydrate‐active enzymes profiles. Resistance ecological niches (meconium, colostrum, soil, wastewater) were unique, resembling maternal sources. Mobile genetic elements (MGEs), found soil wastewater, played an important role mediating these interactions. Multidrug consistently emerged most significant form at both metagenome metatranscriptome levels. Several antibiotic classes showed higher proportions level than DNA level, indicating that even low‐abundance gene groups can have a considerable influence through high expression. This study broadens our understanding dissemination livestock systems, providing foundation for developing future preventive control strategies.

Язык: Английский

Процитировано

0