Analysis and prevention of microbial degradation of shadow puppetry artifacts preserved in the National Shadow Puppetry Museum in Chengdu

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Июнь 3, 2025

Shadow puppetry, an integral and crucial component of China’s intangible cultural heritage, currently faces the significant threat microbial degradation. This is primarily due to organic materials used in its artifacts. study centers on shadow puppets housed National Puppetry Museum Chengdu. By employing Scanning Electron Microscopy (SEM) high-throughput sequencing techniques, it has revealed a diverse array co-existing microorganisms surfaces these puppets. These include species from genera such as Aspergillus , Streptomyces Nocardiopsis Pseudomonas Saccharopolyspora among others. Eleven were successfully isolated, wherein four identified predominant: sp. WH. S-B1, S-B2, S-B6, fumigatus S-F2. Notably, strains demonstrated ability degrade collagen. The antimicrobial experiment results indicated that 0.3% isothiazolinone-based agents BC01 50 mg/mL carvacrol exhibited certain degree activity against predominant strains. Overall, this research provides robust foundation for conservation puppet It does so by thoroughly analyzing mechanisms degradation screening effective agents.

Язык: Английский

Insight into the microbial diversity and community in the sacrificial pits of Sanxingdui site (Sichuan, China)

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Дек. 10, 2024

Introduction The Sanxingdui site (Sichuan, China) is the typical representative of ancient Shu culture, which lasts from late Neolithic to early Western Zhou. sacrificial pits are located in core region site, and numerous artifacts unearthed including ivory, seashells, bronzes, pottery, jade, stone, gold, bone, horn products. function buried has always been focus, but microbiome around attracts less attention. Recently, ivory soil just identified; however, other never studied. In term unique perspective for interpretation archaeological issues, study was carried out for: (1) microbial diversity community lower layer pits, (2) potential biodeterioration behavior organic inorganic relics, (3) impact burial activities different on microbiome. Methods There were 45 samples three 12 raw inside or outside pit sampling site. genomes then identified analyzed using next-generation high-throughput sequencing. Results represented bacterial phyla Proteobacteria, Actinobacteriota, GAL15, Chloroflexi, Acidobacteriota, Methylomirabilota, Thermoplasmatota, Crenarchaeota, Gemmatimonadota, Firmicutes, fungal Ascomycota, Mortierellomycota, Basidiomycota. Further functional analysis found that genera Sphingopyxis , Limnobacter Streptomyces Cladosporium Acremonium Mortierella concerned with degradation matter, while Pseudomonas, Arthrobacter Variovorax Aspergillus Penicillium might be related biocorrosion bronzes. addition, composition principal co-ordinate (PCoA) demonstrated significant differences structure between also pits. Discussion It important understand according results community. combination microbiology archaeology will shed light issues human behaviors.

Язык: Английский