Опубликована: Янв. 1, 2024
Язык: Английский
Frontiers in Microbiology, Год журнала: 2025, Номер 15
Опубликована: Янв. 15, 2025
The contrasting response of AOA, AOB, and comammox Nitrospira amoA transcript abundance to temperature, moisture, nitrogen was investigated using soil microcosms. treatments were selected represent conditions typically found in a New Zealand (NZ) dairy farm. AOB dominated all synthetic urine treated soils. Peak positively correlated with estimated ammonia availability. While gDNA nitrification rate trends similar. AOA strongly influenced by temperature. At 20°C, peak averaged over 1 order magnitude higher than at 8°C. Within the community member Nitrosocosmicus clade ammonium concentrations. presence relative increase an high environment poses interesting contrast current scientific opinion NZ. Comammox showed no correlation moisture. This suggests that previously associations are more complex originally thought. Further research is required determine drivers moisture environment. Overall, these results indicate main farm
Язык: Английский
Процитировано
0
Agronomy, Год журнала: 2025, Номер 15(2), С. 255 - 255
Опубликована: Янв. 21, 2025
The application of nitrification inhibitors (NIs) is an effective way to reduce soil nitrogen (N) losses and increase crop N uptake. Yet, the efficacy NIs commonly varies with dosages, systems environmental conditions. Hence, clarifying suitable type dosage extremely important for structuring best management regime at a regional scale. Here, based on microcosm experiments, we evaluated influence three widely used [Dicyandiamide, DCD; 3,4-Dimethylpyrazole phosphate, DMPP; 2-chloro-6-(trichloromethyl) pyridine, Nitrapyrin] activity intensively cultivated greenhouse soil. results showed that both DCD DMPP imposed transient inhibition (less than five days) regardless dosages applied, and, contrary, Nitrapyrin presented persistent suppression, longer duration action by higher dosage. Accordingly, incorporation 2% applied rate (w/w) recommendable local intensive production. Further, assessed various (0%, 0.25%, 0.5%, 5%) abundance community groups ammonia oxidizers [i.e., ammonia-oxidizing archaea (AOA), bacteria (AOB) completely (Comammox Nitrospira)] qPCR high-throughput amplicon sequencing. strongly lowered AOB Comammox Nitrospira abundances their richness even lowest also significantly altered structure all tested oxidizers, average relative some major members (i.e., Nitrososphaerales Clade Nitrososphaera, A, Nitrosospira briensis Clade, multiformis A.2 A-associated) obviously responded incorporation. Overall, our findings indicated were more sensitive as compared AOA. obtained here highlight importance optimizing fertilization in vegetable inhibits probably suppressing level herein.
Язык: Английский
Процитировано
0
Journal of Water Process Engineering, Год журнала: 2025, Номер 75, С. 107918 - 107918
Опубликована: Май 10, 2025
Язык: Английский
Процитировано
0
Soil Biology and Biochemistry, Год журнала: 2025, Номер 208, С. 109848 - 109848
Опубликована: Май 10, 2025
Язык: Английский
Процитировано
0
The Science of The Total Environment, Год журнала: 2024, Номер 948, С. 174839 - 174839
Опубликована: Июль 16, 2024
Язык: Английский
Процитировано
1
Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(9)
Опубликована: Авг. 19, 2024
ABSTRACT Phosphoglucose isomerase (PGI) links glycolysis, the pentose phosphate pathway (PPP), and synthesis of cell wall precursors in fungi by facilitating reversible conversion between glucose-6-phosphate (Glc6p) fructose-6-phosphate (Fru6P). In a previous study, we established essential role PGI biosynthesis opportunistic human fungal pathogen Aspergillus fumigatus , highlighting its potential as therapeutic target. this conducted transcriptomic analysis discovered that Δ pgi mutant exhibited enhanced reduced PPP, an upregulation precursor pathways. Phenotypic revealed defective protein N -glycosylation mutant, notably absence glycosylated virulence factors DPP V catalase 1. Interestingly, defects were not accompanied activation MpkA-dependent integrity (CWI) signaling pathway. Instead, nitrate assimilation was activated stimulating glutamine providing amino donors for chitin biosynthesis. Blocking severely impaired growth crucial rescuing defects. This study unveils connection nitrogen compensation A. . IMPORTANCE is common serious causes variety diseases. Given limited availability antifungal drugs increasing drug resistance, it imperative to understand fungus’ survival mechanisms effective control infections. Our highlighted maintaining integrity, sugar homeostasis, virulence. The present further illuminates involvement -glycosylation. Furthermore, research reveals pathway, rather than canonical CWI compensates deficiencies mutant. These findings offer valuable insights into novel adaptation mechanism address defects, which could hold promise treatment
Язык: Английский
Процитировано
1
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0