The dynamics of microbial community structure and metabolic function in different parts of cigar tobacco leaves during air-curing DOI Creative Commons

Wenlong Li,

Jun Yu, Hao Li

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Дек. 12, 2024

Air-curing is the initial step in processing of cigar tobacco leaves. However, dynamics microbial community and metabolic functions different parts leaves during this process remain largely unclear. In study, amplicon-based high-throughput sequencing revealed that Pseudomonas (9.0 to 29.9%) Sphingomonas (0.5 13.8%) were dominant bacterial genera early stages air-curing, while Pantoea (1.7–90.4%) became predominant after air-curing. Microbial diversity analysis indicated species richness significantly higher fresh leaf withering periods. Functional prediction based on PICRUSt2 suggested communities middle exhibited abundances pathways related carbohydrates amino acids than those upper leaves, potentially leading formation more flavor compounds. The volatile compounds detected air-curing by HS-SPME-GC–MS, with alkaloids esters being most prominent, although their accumulation periods differed across parts. Furthermore, PLS-DA, 17 38 changed components identified respectively. Finally, potential relationships between characteristic microbes explored Spearman correlation coefficient. It was found multiple bacteria such as Rhodanobacter , Gemmatimonas Ramlibacter present significant positive correlations 3,3-dimethylacrylic acid, phenylacetone, 2,3-butanedione, geranylacetone, promoting process. This study provides scientific insights into role microorganisms offers a basis for screening specific functional improve stabilize future.

Язык: Английский

Diversity of microbial communities in cigar filler leaves with different initial water contents analyzed based on high-throughput sequencing technology DOI Creative Commons

Yumeng Gao,

Yihui Wang, Benxiang Hou

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 7, 2025

To study the composition and succession of bacterial fungal communities during fermentation cigar filler leaves with varying initial water contents, high-throughput sequencing technology was used to sequence 16SrRNA genes ITS1 from tobacco leaf samples. This followed by analyses microbial α-diversity, community structure, function prediction based on data. The diversity richness decreased over time under different content conditions. Among 18 samples, predominant phyla identified were Proteobacteria, Firmicutes, Actinobacteria, Ascomycota, Basidiomycota, leading genera being Staphylococcus, Sphingomonas, Methylobacterium-Methylorubrum, Pseudomonas, Humicola. Functional predictions for bacteria revealed their primary involvement in carbohydrate, lipid, amino acid metabolism. influenced structure relative abundance fermentation. While exhibited a similar structural composition, there notable differences abundance. functional results PICRUSt indicated that predicted species among samples minimal, whereas variations more pronounced across stages contents.

Язык: Английский

Процитировано

0
The dynamics of microbial community structure and metabolic function in different parts of cigar tobacco leaves during air-curing DOI Creative Commons

Wenlong Li,

Jun Yu, Hao Li

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Дек. 12, 2024

Air-curing is the initial step in processing of cigar tobacco leaves. However, dynamics microbial community and metabolic functions different parts leaves during this process remain largely unclear. In study, amplicon-based high-throughput sequencing revealed that Pseudomonas (9.0 to 29.9%) Sphingomonas (0.5 13.8%) were dominant bacterial genera early stages air-curing, while Pantoea (1.7–90.4%) became predominant after air-curing. Microbial diversity analysis indicated species richness significantly higher fresh leaf withering periods. Functional prediction based on PICRUSt2 suggested communities middle exhibited abundances pathways related carbohydrates amino acids than those upper leaves, potentially leading formation more flavor compounds. The volatile compounds detected air-curing by HS-SPME-GC–MS, with alkaloids esters being most prominent, although their accumulation periods differed across parts. Furthermore, PLS-DA, 17 38 changed components identified respectively. Finally, potential relationships between characteristic microbes explored Spearman correlation coefficient. It was found multiple bacteria such as Rhodanobacter , Gemmatimonas Ramlibacter present significant positive correlations 3,3-dimethylacrylic acid, phenylacetone, 2,3-butanedione, geranylacetone, promoting process. This study provides scientific insights into role microorganisms offers a basis for screening specific functional improve stabilize future.

Язык: Английский

Процитировано

0