RNA-binding is an ancient trait of the Annexin family DOI Creative Commons
Sudarshan Patil, Vipul Panchal,

Trude Røstbø

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2023, Номер 11

Опубликована: Июнь 15, 2023

Introduction: The regulation of intracellular functions in mammalian cells involves close coordination cellular processes. During recent years it has become evident that the sorting, trafficking and distribution transport vesicles mRNA granules/complexes are closely coordinated to ensure effective simultaneous handling all components required for a specific function, thereby minimizing use energy. Identification proteins acting at crossroads such events will ultimately provide mechanistic details Annexins multifunctional involved variety processes associated with Ca 2+ -regulation lipid binding, linked operation both endocytic exocytic pathways. Furthermore, certain have been implicated translation. Since Annexin A2 binds mRNAs via its core structure is also present mRNP complexes, we speculated whether direct association RNA could be common property family sharing highly similar structure. Methods results: Therefore, performed spot blot UV-crosslinking experiments assess binding abilities different Annexins, using annexin c-myc 3′UTRs as well 5′UTR baits. We supplemented data immunoblot detection selected complexes derived from neuroendocrine rat PC12 cells. biolayer interferometry was used determine K D Annexin-RNA interactions, which indicated distinct affinities. Amongst these A13 structures A7, A11 bind 3′UTR s nanomolar range. Of only indicating some selectivity. Discussion: oldest members share ability associate RNA, suggesting RNA-binding an ancient trait this protein family. Thus, combined RNA- lipid-binding properties make them attractive candidates participate long-distance membrane regulated by . screening results can thus pave way studies novel context.

Язык: Английский

Unlocking novel therapeutic avenues in glioblastoma: Harnessing 4-amino cyanine and miRNA synergy for next-gen treatment convergence DOI

K Sandhanam,

Tamilanban Thamaraikani,

K. Manasa

и другие.

Neuroscience, Год журнала: 2024, Номер 553, С. 1 - 18

Опубликована: Июнь 27, 2024

Язык: Английский

Процитировано

5
Transcriptomic analysis of intracellular RNA granules and small extracellular vesicles: Unmasking their overlap in a cell model of Huntington’s Disease DOI Creative Commons
Deepti Kailash Nabariya,

Lisa Knüpfer,

Patrick Hartwich

и другие.

Molecular and Cellular Probes, Год журнала: 2025, Номер unknown, С. 102026 - 102026

Опубликована: Март 1, 2025

Huntington's disease (HD) arises from the abnormal expansion of a CAG repeat in HTT gene. The mutant triggers aberrant RNA-protein interactions and translates into toxic aggregate-prone polyglutamine protein. These ineractions also seed formation cytoplasmic liquid-like granules, such as stress granules. Emerging evidence demonstrates that granules formed via liquid-liquid phase separation can mature gel-like inclusions persist within cell may act precursor to aggregates occur patients' tissue. Thus, deregulation RNA is an important component neurodegeneration. Interestingly, both intracellular membrane-less organelles like secretion small extracellular vesicles (sEVs) increase upon under conditions. sEVs are lipid membrane-bound particles secreted all types participate spreading misfolded proteins complexes across central nervous system neurodegenerative diseases HD. In this study, we performed comparative transcriptomic analysis HD model. were isolated inducible Both induced non-induced (control) cells analysed by sequencing. Our between transcriptomics data showed that: (I) share content, (II) several non-coding RNAs translocate (III) composition affected cells. showing common transcripts suggest sEV loading be related. Moreover, found high abundance lncRNAs control samples, with REST regulation, highlighting their potential role pathogenesis selective incorporation sEVs. transcriptome cargo or serve source for diagnostic strategies. For example, disease-specific RNA-signatures biomarker diseases. Therefore, compared our dataset patient blood. However, cell-type specific signature sEV-secreted well variability make it difficult detect these biomarkers

Язык: Английский

Процитировано

0
Unraveling the noncoding RNA landscape in glioblastoma: from pathogenesis to precision therapeutics DOI

K. Sandhanam,

Tamilanban Thamaraikani

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2024, Номер 397(12), С. 9475 - 9502

Опубликована: Июль 15, 2024

Язык: Английский

Процитировано

3
RNA-binding is an ancient trait of the Annexin family DOI Creative Commons
Sudarshan Patil, Vipul Panchal,

Trude Røstbø

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2023, Номер 11

Опубликована: Июнь 15, 2023

Introduction: The regulation of intracellular functions in mammalian cells involves close coordination cellular processes. During recent years it has become evident that the sorting, trafficking and distribution transport vesicles mRNA granules/complexes are closely coordinated to ensure effective simultaneous handling all components required for a specific function, thereby minimizing use energy. Identification proteins acting at crossroads such events will ultimately provide mechanistic details Annexins multifunctional involved variety processes associated with Ca 2+ -regulation lipid binding, linked operation both endocytic exocytic pathways. Furthermore, certain have been implicated translation. Since Annexin A2 binds mRNAs via its core structure is also present mRNP complexes, we speculated whether direct association RNA could be common property family sharing highly similar structure. Methods results: Therefore, performed spot blot UV-crosslinking experiments assess binding abilities different Annexins, using annexin c-myc 3′UTRs as well 5′UTR baits. We supplemented data immunoblot detection selected complexes derived from neuroendocrine rat PC12 cells. biolayer interferometry was used determine K D Annexin-RNA interactions, which indicated distinct affinities. Amongst these A13 structures A7, A11 bind 3′UTR s nanomolar range. Of only indicating some selectivity. Discussion: oldest members share ability associate RNA, suggesting RNA-binding an ancient trait this protein family. Thus, combined RNA- lipid-binding properties make them attractive candidates participate long-distance membrane regulated by . screening results can thus pave way studies novel context.

Язык: Английский

Процитировано

3