Peptides, Год журнала: 2022, Номер 158, С. 170880 - 170880
Опубликована: Сен. 25, 2022
Язык: Английский
Current Research in Biotechnology, Год журнала: 2023, Номер 5, С. 100121 - 100121
Опубликована: Янв. 1, 2023
Antimicrobial peptides (AMPs) are natural biopolymers in the host defense system. AMPs display multiple functions against bacterial, fungal, and viral infection, as well anticancer properties. promising antiviral agents. The average size, net charge, hydrophobicity residue percentage of these Peptide Database (APD) 26 amino acids, 3.5 (+), 48 %, respectively. However, pharmacokinetic disadvantages such low proteolytic chemical stability, high cytotoxicity hemolytic activity, salt sensitivity inhibit their clinical application. Given effects on features, circumventing drawbacks improving efficacy critically important for structure acid components can be modified by several methods, including stabilization, hybridization, cyclization, fragmentation, multimerization, alteration conjugation or ligation. Furthermore, nanotechnologies effective tools strategies to improve reduce side effects. For example, melittin-nano its therapeutic minimize nonspecific cytotoxicity. Clinical trials waiting evaluate delivery system novel treatments.
Язык: Английский
Процитировано
27
Pharmaceuticals, Год журнала: 2023, Номер 16(9), С. 1281 - 1281
Опубликована: Сен. 11, 2023
The rise in antibiotic-resistant strains of clinically important pathogens is a major threat to global health. World Health Organization (WHO) has recognized the urgent need develop alternative treatments address growing list priority pathogens. Antimicrobial peptides (AMPs) rank among suggested options with proven activity and high potential be developed into effective drugs. Many AMPs are naturally produced by living organisms protecting host against as part their innate immunity. Mechanisms associated AMP actions include cell membrane disruption, wall weakening, protein synthesis inhibition, interference nucleic acid dynamics, inducing apoptosis necrosis. Acinetobacter baumannii critical pathogen, severe clinical implications have from isolates resistant current antibiotic conventional control procedures, such UV light, disinfectants, drying. Here, we review natural representing primary candidates for new anti-A. drugs post-antibiotic-era present computational tools next generation greater microbicidal reduced toxicity.
Язык: Английский
Процитировано
11
Phytopathology, Год журнала: 2024, Номер 114(5), С. 971 - 981
Опубликована: Фев. 20, 2024
Nodule-specific cysteine-rich (NCR) peptides, encoded in the genome of Mediterranean legume Medicago truncatula (barrelclover), are known to regulate plant−microbe interactions. A subset computationally derived 20-mer peptide fragments from 182 NCR peptides was synthesized identify those with activity against unculturable vascular pathogen associated citrus greening disease, ‘ Candidatus Liberibacter asiaticus’ ( CLas). Grounded a design experiments framework, we evaluated screening pipeline involving three distinct assays: bacterial culture assay crescens, CLas-infected excised leaf assay, and an evaluate effects on acquisition by nymphal stage hemipteran vector Diaphorina citri. inhibits both CLas growth leaves D. Two induced higher levels citri mortality. These findings reveal as new class plant-derived biopesticide molecules control disease.
Язык: Английский
Процитировано
3
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(3), С. 2694 - 2694
Опубликована: Янв. 31, 2023
Medicago truncatula in symbiosis with its rhizobial bacterium partner produces more than 700 nodule-specific cysteine-rich (NCR) peptides diverse physicochemical properties. Most of the cationic NCR have antimicrobial activity and potential to tackle resistance their novel modes action. This work focuses on antibacterial NCR169 peptide derivatives as we previously demonstrated that C-terminal sequence (NCR169C17-38) has antifungal activity, affecting viability, morphology, biofilm formation various Candida species. Here, show NCR169C17-38 substituted are also able kill ESKAPE pathogens such Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli. The replacement two cysteines serines enhanced against most tested bacteria, indicating a disulfide bridge is not required. As tryptophan can play role interaction bacterial membranes thus replaced tryptophans NCR169C17-38C12,17/S modified tryptophans, namely 5-methyl tryptophan, 5-fluoro 6-fluoro 7-aza 5-methoxy synthesis analogs. results demonstrate presence fluorotryptophans significantly enhance without notable hemolytic effect, this finding could be beneficial for further development new AMPs from members family.
Язык: Английский
Процитировано
5
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(21), С. 15934 - 15934
Опубликована: Ноя. 3, 2023
The overuse and misuse of antibiotics have led to the emergence spread multidrug-resistant (MDR), extensively drug-resistant (XDR), pan-drug-resistant (PDR) bacteria strains, usually associated with poorer patient outcomes higher costs. In order preserve usefulness these life-saving drugs, it is crucial use them appropriately, as also recommended by WHO. Moreover, innovative, safe, more effective approaches are being investigated, aiming revise drug treatments improve their pharmacokinetics distribution reduce onset resistance. Globally, burden antimicrobial resistance (AMR), guidelines indications been developed over time, aimed at narrowing diminishing environmental molecules optimizing prescriptions, dosage, times use, well investing resources into obtaining innovative formulations better pharmacokinetics, pharmacodynamics, therapeutic results. This has development new nano-formulations delivery vehicles, characterized unique structural properties, biocompatible natures, targeted activities such state-of-the-art phospholipid particles generally grouped liposomes, virosomes, functionalized exosomes, which represent an attractive approach. Liposomes virosomes chemically synthesized carriers that utilize phospholipids whose nature predetermined based on a long track record systems. Exosomes vesicles naturally released cells, lipids present in cellular membranes only, therefore, highly biocompatible, investigations system having recent origin. review will summarize state art microvesicle research, useful tools tackle threat antibiotic
Язык: Английский
Процитировано
5
Probiotics and Antimicrobial Proteins, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 18, 2024
Язык: Английский
Процитировано
1
Trends in Plant Science, Год журнала: 2022, Номер 28(2), С. 125 - 127
Опубликована: Ноя. 26, 2022
Язык: Английский
Процитировано
2
SSRN Electronic Journal, Год журнала: 2022, Номер unknown
Опубликована: Янв. 1, 2022
Food spoilage poses a huge threat to human life and health. This study intends identify novel antifungal peptides avoid food drink spoilage. We conducted ab initio design based on the recently improved database filtering technology. Two potent with 15 21 amino acids were assembled by extracting most probable parameters encoded in over 1200 naturally occurring antimicrobial peptide (APD). Inspired acid signature of 19 θ-defensins, we then diversified hydrophobic two designed peptides. Remarkably, variants showed not only enhanced activity against pathogen Zygosaccharomyces bailii but also reduced cytotoxicity host cells (i.e., high selectivity). In addition, retained between pH 5.6 6.3 or after autoclave. Different from enzyme inhibitory fluconazole, fluorescence spectroscopy revealed membrane targeting our peptides, rendering it more difficult for yeast develop resistance. These results indicate that may be useful candidates developing preservatives.
Язык: Английский
Процитировано
0
Peptides, Год журнала: 2022, Номер 158, С. 170880 - 170880
Опубликована: Сен. 25, 2022
Язык: Английский
Процитировано
0