Functional Characterization of a Female-Biased Chemoreceptor of the Codling Moth (Cydia pomonella) Responding to Aldehydes and Other Volatile Compounds
Journal of Chemical Ecology,
Год журнала:
2025,
Номер
51(2)
Опубликована: Фев. 25, 2025
Язык: Английский
Candidate Pheromone Receptors of the Red-Belted Clearwing Moth Synanthedon myophaeformis Bind Pear Ester and Other Semiochemicals
Agriculture,
Год журнала:
2025,
Номер
15(10), С. 1112 - 1112
Опубликована: Май 21, 2025
The
red-belted
clearwing
moth
Synanthedon
myophaeformis
is
a
deleterious
pest
of
apple
orchards,
wherein
the
larvae
bore
tree
bark,
resulting
in
reduced
fitness
and
ultimately
death.
main
control
strategies
this
still
rely
on
use
pesticides,
while
alternative
agronomic
methods
for
its
coexist,
with
application
pheromone
(Z,Z)-3,13-octadecadien-1-yl
acetate.
Until
now,
molecular
bases
chemosensory
systems
have
been
less
explored.
With
aim
to
identify
novel
ligands
that
may
interfere
behaviour
S.
myophaeformis,
study,
we
isolated
functionally
characterised
some
key
odorant
receptors
(ORs)
by
selecting
paralogues
from
two
subgroups
Lepidopteran
receptor
(PR)
clade:
OR3
subgroup
(OR3.1
OR3.4)
OR22
(OR22.1
OR22.4).
We
generated
transgenic
D.
melanogaster
expressing
SmyoORs
ab3A
neurons,
which
approached
single
sensillum
recording
(SSR).
Among
these
ORs,
deorphanized
SmyoOR3.4
previously
identified
orthologues
codling
Cydia
pomonella,
including
pear
ester
ethyl-(E,Z)-2,4-decadienoate,
methyl
analogue
methyl-(E,Z)-2,4-decadienote,
unsaturated
aldehyde
(Z)-6-undecenal.
approach,
also
wide
pattern
activation
SmyoOR22.4
several
apple-emitted
ligands.
Despite
fact
combining
SSR
gas
chromatography
(GC-SSR)
did
not
unveil
compounds
present
headspace
apples,
GC-SSR
unveiled
enhancement
spiking
at
nanogram
doses
both
ester,
For
first
time,
study
ORs
as
possible
semiochemicals
add
ongoing
pest.
Язык: Английский
Identification of the trail-following pheromone receptor in termites
Опубликована: Окт. 21, 2024
Chemical
communication
is
the
cornerstone
of
eusocial
insect
societies
since
it
mediates
social
hierarchy,
division
labor,
and
concerted
activities
colony
members.
The
chemistry
pheromones
received
considerable
attention
in
both
major
groups
insects,
Hymenoptera
termites.
By
contrast,
current
knowledge
on
molecular
mechanisms
pheromone
detection
by
odorant
receptors
(ORs)
limited
to
hymenopteran
insects
no
OR
was
yet
functionally
characterized
termites,
oldest
clade.
Here,
we
present
first
deorphanization
Using
data
from
antennal
transcriptome
genome
termite
Prorhinotermes
simplex
(Rhinotermitidae),
selected
4
candidate
sequences,
expressed
them
Empty
Neuron
Drosophila
,
using
single
sensillum
recording
(SSR)
a
panel
semiochemicals.
In
one
ORs,
PsimOR14,
succeeded
obtaining
strong
reliable
responses
main
component
P.
trail-following
pheromone,
monocyclic
diterpene
neocembrene.
PsimOR14
showed
narrow
tuning
neocembrene;
only
additional
compound
out
72
tested
(geranylgeraniol)
generated
non-negligible
responses.
Subsequently,
used
SSR
workers
identify
olfactory
specifically
responding
neocembrene,
thus
likely
expressing
.
We
report
homology-based
modelling
neocembrene
binding
show
how
different
ligands
impact
receptor
dynamicity
dynamics
simulations.
Finally,
demonstrate
that
significantly
more
than
soldiers,
which
correlates
with
higher
sensitivity
Язык: Английский
Identification of the trail-following pheromone receptor in termites
Опубликована: Окт. 21, 2024
Chemical
communication
is
the
cornerstone
of
eusocial
insect
societies
since
it
mediates
social
hierarchy,
division
labor,
and
concerted
activities
colony
members.
The
chemistry
pheromones
received
considerable
attention
in
both
major
groups
insects,
Hymenoptera
termites.
By
contrast,
current
knowledge
on
molecular
mechanisms
pheromone
detection
by
odorant
receptors
(ORs)
limited
to
hymenopteran
insects
no
OR
was
yet
functionally
characterized
termites,
oldest
clade.
Here,
we
present
first
deorphanization
Using
data
from
antennal
transcriptome
genome
termite
Prorhinotermes
simplex
(Rhinotermitidae),
selected
4
candidate
sequences,
expressed
them
Empty
Neuron
Drosophila
,
using
single
sensillum
recording
(SSR)
a
panel
semiochemicals.
In
one
ORs,
PsimOR14,
succeeded
obtaining
strong
reliable
responses
main
component
P.
trail-following
pheromone,
monocyclic
diterpene
neocembrene.
PsimOR14
showed
narrow
tuning
neocembrene;
only
additional
compound
out
72
tested
(geranylgeraniol)
generated
non-negligible
responses.
Subsequently,
used
SSR
workers
identify
olfactory
specifically
responding
neocembrene,
thus
likely
expressing
.
We
report
homology-based
modelling
neocembrene
binding
show
how
different
ligands
impact
receptor
dynamicity
dynamics
simulations.
Finally,
demonstrate
that
significantly
more
than
soldiers,
which
correlates
with
higher
sensitivity
Язык: Английский