Unveiling the role of ASPP1 in cancer progression: pan-cancer bioinformatics and experimental validation in colorectal cancer
Frontiers in Oncology,
Год журнала:
2025,
Номер
14
Опубликована: Янв. 2, 2025
The
Apoptosis-Stimulating
Protein
of
P53
(ASPP)
family
contributes
to
apoptosis
regulation
and
tumor
suppression,
with
ASPP1
influencing
processes
like
cancer
cell
proliferation,
invasion,
migration.
Its
expression
varies
across
types,
suggesting
a
potential
role
in
oncogenesis.
This
study
investigates
ASPP1's
various
cancers
using
comprehensive
bioinformatics
approach.
Data
were
extracted
from
public
resources,
including
Cancer
Genome
Atlas
(TCGA),
GTEx,
the
Human
Atlas,
analyzed
via
tools
such
as
cBioPortal,
GEPIA,
TIMER2.
Statistical
network
analyses
performed
R,
Cytoscape,
Hiplot.
function
colorectal
was
further
explored
through
vitro
assays,
qRT-PCR,
Western
blotting,
colony
formation,
Transwell,
wound
healing.
exhibited
significant
variability
different
marked
associations
patient
outcomes,
particularly
overall
survival
(OS)
disease-specific
(DSS)
several
types.
In-depth
protein-protein
interaction
(PPI)
analysis
revealed
involvement
progression
networks.
Functional
enrichment
linked
key
apoptotic
signaling
pathways
transcriptional
regulatory
processes,
underscoring
its
impact
on
biology.
Additionally,
correlates
immune
infiltration
patterns,
cancer-associated
fibroblasts
markers,
roles
response
modulation.
In
assays
lines
significantly
lower
levels
compared
normal
colon
cells
(HCM460),
overexpression
experiments
showed
reduction
migration
abilities.
These
cellular
findings
align
predictions,
highlighting
suppressor
metastatic
traits
cancer.
highlights
forecasting
biomarker
potentially
other
cancers.
support
biology,
regarding
proliferation
potential,
establishing
foundation
for
investigation
into
therapeutic
relevance.
Язык: Английский
Impact of the Immune Landscape in Follicular Lymphoma: Insights into Histological Transformation in the Rituximab Era
Cancers,
Год журнала:
2024,
Номер
16(20), С. 3553 - 3553
Опубликована: Окт. 21, 2024
Follicular
lymphoma
(FL)
presents
significant
clinical
heterogeneity,
with
some
patients
experiencing
transformation
into
an
aggressive
disease,
a
key
contributor
to
FL-related
mortality.
Based
on
gene
expression
profiles,
this
study
aimed
provide
insights
immunological
differences
associated
transformation.
Язык: Английский
G Protein-coupled Estrogen Receptor 1 (GPER1) Regulates Expression ofSERPINE1/PAI-1 and Inhibits Tumorigenic Potential of Cervical Squamous Cell Carcinoma CellsIn Vitro
Cancer Genomics & Proteomics,
Год журнала:
2024,
Номер
22(1), С. 13 - 23
Опубликована: Дек. 27, 2024
Background/Aim:
G
protein-coupled
estrogen
receptor
1
(GPER1)
appears
to
play
a
tumor-suppressive
role
in
cervical
squamous
cell
carcinoma
(CSCC)GPER1
suppression
leads
significantly
increased
expression
of
serpin
family
E
member
(SERPINE1)/protein
plasminogen
activator
inhibitor
type
(PAI-1).
The
question
arises,
what
does
SERPINE1/PAI-1
GPER1-dependent
tumorigenic
potential
CSCC.
Materials
and
Methods:
SiHa
C33A
CSCC
cells
were
treated
with
GPER1
agonist
G1
or
antagonist
G36.
was
suppressed
by
RNAi
success
confirmed
RT-qPCR.
Protein
PAI-1
quantified
Western
blot.
Viability
analyzed
using
resazurin
assay,
while
migration
investigated
gap
closure.
Colony
tumor
sphere
formation
used
test
clonogenicity.
Results:
After
treatment,
viability
remained
unchanged.
Cell
dose-dependently
reduced.
formed
fewer
smaller
colonies
as
well
spheroids.
Furthermore,
treatment
led
decreased
SERPINE1/PAI-1,
blockade
G36
resulted
expression.
RNAi,
unaffected;
however,
formed,
spheroids
developed.
unaffected.
Conclusion:
Activation
reduces
clonogenicity
suppresses
SERPINE1/PAI-1.
Suppression
potential.
may
be
suitable
target
for
However,
not
appear
the
decisive
factor
GPER1-regulated
migration.
Язык: Английский