Опубликована: Янв. 1, 2024
Язык: Английский
Neuroscience, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
1
Ecological Informatics, Год журнала: 2024, Номер unknown, С. 102893 - 102893
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
7
Neuroscience & Biobehavioral Reviews, Год журнала: 2023, Номер 155, С. 105475 - 105475
Опубликована: Ноя. 22, 2023
The brain is a remarkably complex organ responsible for wide range of functions, including the modulation emotional states and movement. Neuronal circuits are believed to play crucial role in integrating sensory, cognitive, information ultimately guide motor behavior. Over years, numerous studies employing diverse techniques such as electrophysiology, imaging, optogenetics have revealed network neural involved regulation or processes. Emotions can exert substantial influence on performance, encompassing both everyday activities pathological conditions. aim this review explore how shape movements by connecting processing circuits. We first provide comprehensive overview impact different control humans rodents. In line with behavioral studies, we set out identify emotion-related structures capable modulating output, behaviorally anatomically. extensively connected system. These drive behavior, essential survival, but also continuously ongoing summary, investigation intricate relationship between emotion movement offers valuable insights into human opportunities enhance holds promise improving mental physical health. This integrates findings from multiple scientific approaches, anatomical tracing, circuit-based dissection, conducted animal subjects. By incorporating these methodologies, present current understanding physiological
Язык: Английский
Процитировано
9
MethodsX, Год журнала: 2024, Номер 12, С. 102721 - 102721
Опубликована: Апрель 17, 2024
In this procedure we have included an open-source method for a customized operant chamber optimized long-term miniature microscope (miniscope) recordings. •The miniscope box is designed to function with custom or typical med-associates style accessories (e.g., houselights, levers, etc.).•The majority of parts can be directly purchased which minimizes the need skilled and time-consuming labor.•We include designs estimated pricing single but it recommended build these in larger batches efficiently utilize bulk ordering certain components.
Язык: Английский
Процитировано
3
Current Protocols, Год журнала: 2025, Номер 5(1)
Опубликована: Янв. 1, 2025
Abstract In vivo calcium imaging in freely moving rats using miniscopes provides valuable information about the neural mechanisms of behavior real time. A gradient index (GRIN) lens can be implanted deep brain structures to relay activity from single neurons. While such procedures have been successful mice, few reports provide detailed for surgery and long‐term rats, which are better suited studying complex human behaviors. We present a robotic surgical protocol same‐day virus injection GRIN implantation into rat nucleus accumbens core. Our procedure utilizes direct insertion without tissue aspiration produces quality image retention months recording. also describe daily recording strategies minimize damage promote imaging. Finally, we custom protective eliminate need remove between sessions. This methodology protects repeated isoflurane exposure ensures consistent focal plane entirety experiment. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1 : Craniotomy 2 Virus 3 4 Baseplate mounting assessment anesthetized 5 Assessment awake, behaving Support Protective miniscope cone fabrication Miniscope cable
Язык: Английский
Процитировано
0
Addiction Neuroscience, Год журнала: 2024, Номер 11, С. 100154 - 100154
Опубликована: Апрель 16, 2024
In humans experiencing substance use disorder (SUD), abstinence from drug is often motivated by a desire to avoid some undesirable consequence of further use: health effects, legal ramifications, etc. This process can be experimentally modeled in rodents training and subsequently punishing an operant response context-induced reinstatement procedure. Understanding the biobehavioral mechanisms underlying punishment learning critical understanding both relapse individuals with SUD. To date, most investigations into neural following have utilized discrete loss-of-function manipulations that do not capture ongoing changes circuitry related punishment-induced behavior change. Here, we describe two-pronged approach analyzing using miniature fluorescence microscopes deep algorithms. We review recent advancements techniques consider target circuit.
Язык: Английский
Процитировано
2
GigaScience, Год журнала: 2022, Номер 12
Опубликована: Дек. 28, 2022
Abstract The importance of effective research data management (RDM) strategies to support the generation Findable, Accessible, Interoperable, and Reusable (FAIR) neuroscience grows with each advance in acquisition techniques methods. To maximize impact diverse strategies, multidisciplinary, large-scale consortia face a number unsolved challenges RDM. While open science principles are largely accepted, it is practically difficult for researchers prioritize RDM over other pressing demands. implementation coherent, executable plan spanning animal, human, clinical studies becoming increasingly challenging. Here, we present an strategy implemented Heidelberg Collaborative Research Consortium. Our consortium combines basic populations (animals humans) produces highly heterogeneous multimodal (e.g., neurophysiology, neuroimaging, genetics, behavior). We concrete initiating early-stage FAIR collaborative consortia, focus on sustainable solutions that incentivize incremental while respecting research-specific requirements.
Язык: Английский
Процитировано
7
MethodsX, Год журнала: 2024, Номер 13, С. 102921 - 102921
Опубликована: Авг. 16, 2024
Язык: Английский
Процитировано
1
Biomedicines, Год журнала: 2022, Номер 11(1), С. 57 - 57
Опубликована: Дек. 26, 2022
Grounding is a therapeutic technique that involves doing activities “ground” or electrically reconnect us to the earth. The physiological effects of grounding have been reported from variety perspectives such as sleep pain. However, its anti-stress efficacy relatively unknown. present study investigated stress-related behavioral earthing mat and neurohormonal mechanisms in Sprague–Dawley male rat. Rats were randomly divided into four groups: naïve normal (Normal), 21 days immobilization stressed (Control), + for 7 (A7) (A21) group. depressive-and anxiety like behaviors measured by forced swimming test (FST), tail suspension (TST) elevated plus maze (EPM). Using immunohistochemistry, expression corticotrophin-releasing factor (CRF) c-Fos immunoreactivity analyzed brain. In EPM, time spent open arm groups was significantly increased compared Control group (p < 0.001), even though there without among FST TST. CRF immunoreactive neurons markedly decreased Overall, reduced stress-induced changes These results suggest may potential improve responses via regulation corticotrophinergic system.
Язык: Английский
Процитировано
6
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Март 4, 2024
Abstract Introduction Fragile X Syndrome (FXS), caused by mutations in the Fmr1 gene, is a neurodevelopmental condition linked to cognitive and behavioral differences, including atypical interactions heightened anxiety-like responses social settings. Previous studies that used FXS mouse models focused mostly on adulthood but not behaviors displayed juvenile mice. This especially concerning considering extensive body of research conducted children adolescents with FXS, which indicates dynamic changes from childhood adulthood. Thus, it crucial study how affects during stage, both female male In this study, we compared behavior knockout (KO) mice, model wild-type (WT) mice C57BL/6J strain. Methods We examined at postnatal day 40 (P40). Social interaction tests were carried out using two same sex genotype, who previously had encountered each other, allowed interact freely an empty chamber for 10 minutes. Their recorded overhead camera Key such as head, body, anogenital sniffing, physical touch, well distance traveled, analyzed recently developed marker-less tracking software. Results showed pronounced genotype differences between females males WT KO genotypes. Overall, found all parameters measured. Notably, engaged more events behavior. On other hand, there no significant observed males. Discussion These results highlight importance arise stage model, suggesting early post-natal phases may be promising window therapeutic interventions.
Язык: Английский
Процитировано
0