Predicting visual function by interpreting a neuronal wiring diagram

Nature, Год журнала: 2024, Номер 634(8032), С. 113 - 123

Опубликована: Окт. 2, 2024

Язык: Английский

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Single-cell transcriptome profiles of Drosophila fruitless-expressing neurons from both sexes

eLife, Год журнала: 2023, Номер 12

Опубликована: Фев. 1, 2023

Drosophila melanogaster reproductive behaviors are orchestrated by fruitless neurons. We performed single-cell RNA-sequencing on pupal neurons that produce sex-specifically spliced fru transcripts, the P1-expressing Uniform Manifold Approximation and Projection (UMAP) with clustering generates an atlas containing 113 clusters. While male female overlap in UMAP space, more than half clusters have sex differences neuron number, nearly all display sex-differential expression. Based examination of enriched marker genes, we annotate as circadian clock neurons, mushroom body Kenyon cell neurotransmitter- and/or neuropeptide-producing, those express doublesex . Marker gene analyses also show genes encode members immunoglobulin superfamily adhesion molecules, transcription factors, neuropeptides, neuropeptide receptors, Wnts unique patterns expression across In vivo spatial links to examined. A functional analysis P1 shows they dimorphic roles activity period length. Given most comprised indicates sexes common programs. Sex-specific is overlaid this program, build potential for vastly different sex-specific behaviors.

Язык: Английский

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Microbiota-derived acetylcholine can promote gut motility in Drosophila melanogaster

Philosophical Transactions of the Royal Society B Biological Sciences, Год журнала: 2024, Номер 379(1901)

Опубликована: Март 18, 2024

The gut microbiota is crucial for intestinal health, including gastrointestinal (GI) motility. How commensal bacterial species influence GI motility has not been fully elucidated. A major factor of the contraction promoting propulsive movement orally ingested materials. Here, we developed a method to monitor and quantify contractions in living Drosophila melanogaster larvae. We found that culture medium an isolated strain Lactiplantibacillus plantarum Lsi promoted vivo , which was observed Leuconostoc sp. Leui nor Acetobacter persici Ai medium. To identify bacteria-derived metabolites, performed metabolome analysis media by liquid chromatography-tandem mass spectrometry (LC–MS/MS). Of 66 metabolites detected, some changed species-specific manner. Among them, acetylcholine specifically produced L. plantarum. Feeding exogenous increased frequency contractions, blocked D-tubocurarine, inhibitor nicotinic receptors. In this study, propose mechanism influences This article part theme issue ‘Sculpting microbiome: how host factors determine respond microbial colonization’.

Язык: Английский

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Experimentally induced active and quiet sleep engage non-overlapping transcriptional programs in Drosophila

eLife, Год журнала: 2023, Номер 12

Опубликована: Июнь 14, 2023

Sleep in mammals can be broadly classified into two different physiological categories: rapid eye movement (REM) sleep and slow-wave (SWS), accordingly REM SWS are thought to achieve a set of functions. The fruit fly Drosophila melanogaster is increasingly being used as model understand functions, although it remains unclear if the brain also engages kinds well. Here, we compare commonly approaches for studying experimentally : optogenetic activation sleep-promoting neurons provision drug, gaboxadol. We find that these sleep-induction methods have similar effects on increasing duration, but divergent activity. Transcriptomic analysis reveals drug-induced deep (‘quiet’ sleep) mostly downregulates metabolism genes, whereas ‘active’ upregulates wide range genes relevant normal waking This suggests optogenetics pharmacological induction promote features sleep, which engage sets their respective

Язык: Английский

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Columnar cholinergic neurotransmission onto T5 cells of Drosophila

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

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Neuropharmacology in the Molecular Epoch

Опубликована: Апрель 17, 2025

Язык: Английский

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Molecular organization of central cholinergic synapses

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(17)

Опубликована: Апрель 24, 2025

Synapses have undergone significant diversification and adaptation, contributing to the complexity of central nervous system. Understanding their molecular architecture is essential for deciphering brain’s functional evolution. While nicotinic acetylcholine receptors (nAchRs) are widely distributed across metazoan brains, associated protein networks remain poorly characterized. Using in vivo proximity labeling, we generated proteomic maps subunit-specific nAchR interactomes developing mature Drosophila brains. Our findings reveal a developmental expansion reconfiguration interactome. Proteome profiling with genetic perturbations showed that removing individual subunits consistently triggers compensatory shifts receptor subtypes, highlighting mechanisms synaptic plasticity. We also identified Rho-GTPase regulator Still life (Sif) as key organizer cholinergic synapses, loss Sif disrupting composition structural integrity. These results provide insights into development plasticity advancing our understanding identity conservation divergence.

Язык: Английский

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Molecular Targets of Neurotoxic Insecticides in Apis mellifera

European Journal of Organic Chemistry, Год журнала: 2022, Номер 2022(21)

Опубликована: Март 23, 2022

Abstract The intensive use of insecticides, combined with other factors such as habitat loss, might explain worldwide decrease insect populations documented in the past twenty years. However, due to involvement pest species crop destruction and vector‐borne diseases, insecticides will probably continue be required still for decades. most commercially successful are neurotoxicants acting on ion channels central nervous system affecting thereby cellular excitability synaptic transmission causing paralysis fatality. In this article, we provide an overview voltage‐gated sodium channels, GABA‐gated chloride nicotinic acetylcholine receptors. We summarize current knowledge those from honeybee Apis mellifera discuss possible mode action neurotoxic insecticides.

Язык: Английский

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The cholinergic pathway transmits signals of neuropeptide F to regulate feeding of Ostrinia furnacalis larvae

Pest Management Science, Год журнала: 2023, Номер 79(10), С. 3593 - 3601

Опубликована: Май 15, 2023

Feeding is the basis of animal survival and reproduction. In insects, neuropeptide F (NPF), a homologous polypeptide NPY in vertebrates, plays an important role regulation feeding behavior. However, relatively little has been known about molecular mechanism feeding.In this study, we show that cholinergic pathway very signaling transmission NPF Ostrinia furnacalis larvae, which choline acetyltransferase (ChAT), vesicular acetylcholine transporter (vAChT) presynaptic membrane nicotinic receptor (nAChR) postsynaptic are positively regulated by NPF, while ace1 ace2 encoding acetylcholinesterase (AChE) negatively leading to balance (ACh)-the excitatory transmitter. More, further transmits downstream pathways phosphoInositide-3 kinase (PI3K) cAMP responsive element binding protein (CREB), respectively.The transmission, involved O. larvae via PI3K CREB pathways, respectively. The deexcitation cell or inhibition lead decreases larval amount. © 2023 Society Chemical Industry.

Язык: Английский

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Exploring neonicotinoid effects on Drosophila: insights into olfactory memory, neurotransmission, and synaptic connectivity

Frontiers in Physiology, Год журнала: 2024, Номер 15

Опубликована: Март 14, 2024

Neonicotinoid insecticides, the fastest-growing class in recent decades, interfere with cholinergic neurotransmission by binding to nicotinic acetylcholine receptor. This disruption affects both targeted and non-targeted insects, impairing cognitive functions such as olfaction related behaviors, a particular emphasis on olfactory memory due its ecological impact. Despite persistent presence of these chemicals environment, significant research gaps remain understanding intricate interplay between function, development, neuronal activity, neonicotinoid-induced toxicity. study focuses fruit fly Drosophila melanogaster , chosen for genetic tractability, well-characterized neural circuitry, remarkable parallels bees neurotransmitter systems brain structures. Our aim is establish valuable model organism studying effects neonicotinoids behavior attention associated circuitries. To achieve this aim, we conducted experiments investigate short-term exposure sublethal doses neonicotinoid imidacloprid, mimicking realistic environmental insecticide exposure, formation odor memories. Additionally, evaluated synaptic contacts within mushroom body, primary network insects. results showed impairments flies exposed during adult stage showing more pronounced than larval stage. functional studies revealed decrease intrinsic projection neurons body. Furthermore, another experiment an odor-dependent reduction network. In summary, employing provides robust framework investigating their diverse impacts insect physiology behavior. initiates establishment pivotal exploring influences, shedding light memory, integrity, transmission.

Язык: Английский

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