Characterizing the gut microbiota composition in Taiwanese hypertensive patients using 16S rRNA sequencing analysis DOI Creative Commons
Ming‐Shan Chen,

Shin-Kuang Jiang,

Zhi-Yong Chong

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Сен. 18, 2023

Abstract Objective Hypertension (HTN) is a significant risk factor for cardiovascular and cerebrovascular diseases. Accumulating evidence suggests close relationship between HTN dysbiosis of the gut microbiota. Methods In present study, we recruited 23 patients 17 controls matched demographic characteristics. DNA extracted from fecal samples was subjected to Illumina MiSeq sequencing, targeting V3-V4 region bacterial 16S rRNA gene analysis. We compared diversity composition microbiota two groups. Results The α-diversity in similar that control group. β-diversity analysis showed slight differences microbial used Welch's t -test evaluate difference top 20 ASVs group group, results Tyzzerella significantly increased, while Faecalibacterium decreased also conducted Kruskal-Wallis test compare at various taxonomic levels (phylum, class, order, family, genus, species). revealed reduction abundance , Lachnospiraceae_UCG-004 Coprobacter Alphaproteobacteria Additionally, Linear Discriminant Analysis Effect Size (LEfSe) enrichment Enterobacteriaceae whereas Lachnospiraceae_UCG_004 dominated As exhibited lower according above results, performed PCR using -specific primers analyzed their through agarose gel electrophoresis, confirming reduced addition, Tax4Fun2 employed examine functionality Conclusion studied hypertensive population Taiwan found has patients. This unique alteration may provide insights into pathogenesis aid development novel biomarkers therapeutic targets.

Язык: Английский

Characterizing the gut microbiota composition in Taiwanese hypertensive patients using 16S rRNA sequencing analysis DOI Creative Commons
Ming‐Shan Chen,

Shin-Kuang Jiang,

Zhi-Yong Chong

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Сен. 18, 2023

Abstract Objective Hypertension (HTN) is a significant risk factor for cardiovascular and cerebrovascular diseases. Accumulating evidence suggests close relationship between HTN dysbiosis of the gut microbiota. Methods In present study, we recruited 23 patients 17 controls matched demographic characteristics. DNA extracted from fecal samples was subjected to Illumina MiSeq sequencing, targeting V3-V4 region bacterial 16S rRNA gene analysis. We compared diversity composition microbiota two groups. Results The α-diversity in similar that control group. β-diversity analysis showed slight differences microbial used Welch's t -test evaluate difference top 20 ASVs group group, results Tyzzerella significantly increased, while Faecalibacterium decreased also conducted Kruskal-Wallis test compare at various taxonomic levels (phylum, class, order, family, genus, species). revealed reduction abundance , Lachnospiraceae_UCG-004 Coprobacter Alphaproteobacteria Additionally, Linear Discriminant Analysis Effect Size (LEfSe) enrichment Enterobacteriaceae whereas Lachnospiraceae_UCG_004 dominated As exhibited lower according above results, performed PCR using -specific primers analyzed their through agarose gel electrophoresis, confirming reduced addition, Tax4Fun2 employed examine functionality Conclusion studied hypertensive population Taiwan found has patients. This unique alteration may provide insights into pathogenesis aid development novel biomarkers therapeutic targets.

Язык: Английский

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