Gallic acid showed neuroprotection against endoplasmic reticulum stress in rats

Acta Cirúrgica Brasileira, Год журнала: 2025, Номер 40

Опубликована: Янв. 1, 2025

We aimed to investigate the role of gallic acid treatment on spinal cord tissues after injury (SCI) and its relationship with endoplasmic reticulum (ER) stress by histochemical, immunohistochemical, in-silico techniques. Thirty female Wistar albino rats were divided into three groups: sham, SCI, SCI+gallic acid. SCI was induced dropping a 15-g weight onto exposed T10-T11 segment. The group received 25 mg/kg intraperitoneally daily for one week. Histopathological, silico analyses performed. Histological analysis revealed improved neural cell survival tissue integrity in compared group. Caspase-12 expression significantly increased group, indicating elevated ER apoptosis. Gallic resulted marked reduction caspase-12 neurons, neuroglia, endothelial cells, suggesting decreased stress. exhibits significant neuroprotective effects against cellular damage rat model SCI. apoptotic immune-related pathways which showed regulating caspase-12. These results suggest that may be promising therapeutic agent mitigating secondary post-SCI.

Язык: Английский

Trigonelline exerts its neuroprotective effects in experimental spinal cord injury through modulation of inflammation, apoptosis, and neurotrophic factors

Asian Pacific Journal of Tropical Biomedicine, Год журнала: 2025, Номер 15(1), С. 34 - 42

Опубликована: Янв. 1, 2025

Objective: To assess the protective effects of trigonelline against spinal cord injury (SCI) in rats. Methods: Rats (Sprague-Dawley, male) were randomly assigned to seven groups ( n =15 per group): normal, sham, SCI control (1% DMSO), methylprednisolone (30 mg/kg), and (50, 100, 200 mg/kg). received respective treatment daily for 28 days. was induced by using a temporary aneurysm clip. Behavioral, biochemical, histological analyses performed investigate neuroprotective effect trigonelline. Results: Trigonelline (100 mg/kg) effectively P <0.05) mitigated SCI-induced changes mechano-tactile sensation, allodynia, hyperalgesia, motor nerve conduction velocity. It notably downregulated apoptotic (Bax caspase-3) inflammatory (COX-II) markers, while upregulating Bcl-2 BDNF mRNA expression <0.05). Furthermore, alleviated alterations mitochondrial complex levels, resulting enhanced nicotinamide adenine dinucleotide dehydrogenase, succinate redox activity, cytochrome-C levels. Histological examination tissue indicated that significantly ameliorated damage caused SCI, thereby improving neuronal degeneration, cell infiltration, necrosis. Conclusions: shows properties rats reducing inflammation, stabilizing enzyme complexes, modulating neurotrophic factors. Thus, holds promise as potential agent.

Язык: Английский

Aloperine treatment attenuates acute spinal cord injury by reducing oxidative, inflammatory, and apoptotic responses via PI3K/AKT/NF-κB signaling in a rat contusion model

Neuroscience Letters, Год журнала: 2025, Номер unknown, С. 138203 - 138203

Опубликована: Март 1, 2025

Язык: Английский