Cone bipolar cell synapses generate transient versus sustained signals in parallel ON pathways of the mouse retina DOI Open Access

Sidney P. Kuo,

Wan‐Qing Yu, Prerna Srivastava

и другие.

Опубликована: Авг. 20, 2024

Parallel processing is a fundamental organizing principle in the nervous system, and understanding how parallel neural circuits generate distinct outputs from common inputs key goal of neuroscience. In mammalian retina, divergence cone signals into multiple feed-forward bipolar cell pathways forms initial basis for retinal dedicated to specific visual functions. Here, we used patch-clamp electrophysiology, electron microscopy two photon imaging fluorescent glutamate sensor examine kinetically responses arise transient versus sustained ON alpha RGCs (ON-T ON-S RGCs) mouse retina. We directly compared response properties these with their presynaptic partners, which identified using 3D reconstruction. Different subtypes (type 5i, type 6 7) had indistinguishable light-driven whereas extracellular around RGC dendrites postsynaptic excitatory currents measured ON-T identical stimuli probe cells were distinct. Anatomical examination axon terminals suggests subtype-specific differences size synaptic ribbon-associated vesicle pools may contribute kinetics. Our findings indicate synapses are primary point pathways.

Язык: Английский

ENHANCED RESTORATION OF VISUAL CODE AFTER TARGETING ON BIPOLAR CELLS COMPARED TO RETINAL GANGLION CELLS WITH OPTOGENETIC THERAPY DOI Creative Commons
Jessica Rodgers, Steven Hughes, Aghileh S. Ebrahimi

и другие.

Molecular Therapy, Год журнала: 2025, Номер 33(3), С. 1264 - 1281

Опубликована: Янв. 18, 2025

Язык: Английский

Процитировано

1
Biophysical neural adaptation mechanisms enable artificial neural networks to capture dynamic retinal computation DOI Creative Commons
Saad Idrees, Michael B. Manookin, Fred Rieke

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 16, 2024

Abstract Adaptation is a universal aspect of neural systems that changes circuit computations to match prevailing inputs. These facilitate efficient encoding sensory inputs while avoiding saturation. Conventional artificial networks (ANNs) have limited adaptive capabilities, hindering their ability reliably predict output under dynamic input conditions. Can embedding mechanisms in ANNs improve performance? To answer this question, we develop new deep learning model the retina incorporates biophysics photoreceptor adaptation at front-end conventional convolutional (CNNs). CNNs build on ’Deep Retina,’ previously developed retinal ganglion cell (RGC) activity. include layer outperform CNN models predicting male and female primate rat RGC responses naturalistic stimuli local intensity large ambient illumination. improved predictions result directly from within phototransduction cascade. This research underscores potential using them determine how circuits manage complexities natural are span range light levels.

Язык: Английский

Процитировано

5
Cone bipolar cell synapses generate transient versus sustained signals in parallel ON pathways of the mouse retina DOI Creative Commons

Sidney P. Kuo,

Wan‐Qing Yu, Prerna Srivastava

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 14, 2024

Abstract Parallel processing is a fundamental organizing principle in the nervous system, and understanding how parallel neural circuits generate distinct outputs from common inputs key goal of neuroscience. In mammalian retina, divergence cone signals into multiple feed-forward bipolar cell pathways forms initial basis for retinal dedicated to specific visual functions. Here, we used patch-clamp electrophysiology, electron microscopy two photon imaging fluorescent glutamate sensor examine kinetically responses arise transient versus sustained ON alpha RGCs (ON-T ON-S RGCs) mouse retina. We directly compared response properties these with their presynaptic partners, which identified using 3D reconstruction. Different subtypes (type 5i, type 6 7) had indistinguishable light-driven whereas extracellular around RGC dendrites postsynaptic excitatory currents measured ON-T identical stimuli probe cells were distinct. Anatomical examination axon terminals suggests subtype-specific differences size synaptic ribbon-associated vesicle pools may contribute kinetics. Our findings indicate synapses are primary point pathways.

Язык: Английский

Процитировано

3
Cone bipolar cell synapses generate transient versus sustained signals in parallel ON pathways of the mouse retina DOI Open Access

Sidney P. Kuo,

Wan‐Qing Yu, Prerna Srivastava

и другие.

Опубликована: Авг. 20, 2024

Parallel processing is a fundamental organizing principle in the nervous system, and understanding how parallel neural circuits generate distinct outputs from common inputs key goal of neuroscience. In mammalian retina, divergence cone signals into multiple feed-forward bipolar cell pathways forms initial basis for retinal dedicated to specific visual functions. Here, we used patch-clamp electrophysiology, electron microscopy two photon imaging fluorescent glutamate sensor examine kinetically responses arise transient versus sustained ON alpha RGCs (ON-T ON-S RGCs) mouse retina. We directly compared response properties these with their presynaptic partners, which identified using 3D reconstruction. Different subtypes (type 5i, type 6 7) had indistinguishable light-driven whereas extracellular around RGC dendrites postsynaptic excitatory currents measured ON-T identical stimuli probe cells were distinct. Anatomical examination axon terminals suggests subtype-specific differences size synaptic ribbon-associated vesicle pools may contribute kinetics. Our findings indicate synapses are primary point pathways.

Язык: Английский

Процитировано

1
Enhanced restoration of visual code after targeting on bipolar cells compared to retinal ganglion cells with optogenetic therapy DOI Creative Commons
Jessica Rodgers, Steven Hughes, Aghileh S. Ebrahimi

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 23, 2024

ABSTRACT Optogenetic therapy is a promising vision restoration method where light sensitive opsins are introduced to the surviving inner retina following photoreceptor degeneration. The cell type targeted for opsin expression will likely influence quality of restored vision. However, like-for-like pre-clinical comparison visual responses evoked equivalent in two major targets, ON bipolar (ON BCs) or retinal ganglion cells (RGCs), absent. We address this deficit by comparing stimulus-response characteristics at single unit resolution and dorsal lateral geniculate nucleus (dLGN) retinally degenerate mice genetically engineered express ReaChR Grm6- Brn3c -expressing BC vs RGCs respectively). For both targeting strategies, we find ReaChR-evoked have sensitivity can encode contrast across different background irradiances. Compared BCs, decreased response reproducibility resulted more stereotyped with reduced diversity polarity, temporal frequency tuning. Recording ReaChR-driven visually intact retinas confirmed that RGC-targeted disrupts feature selectivity individual RGCs. Our data show while approaches restore impressive fidelity, produces richer code better approaching wildtype mice.

Язык: Английский

Процитировано

0
Cone bipolar cell synapses generate transient versus sustained signals in parallel ON pathways of the mouse retina DOI Open Access

Sidney P. Kuo,

Wan‐Qing Yu, Prerna Srivastava

и другие.

Опубликована: Авг. 20, 2024

Parallel processing is a fundamental organizing principle in the nervous system, and understanding how parallel neural circuits generate distinct outputs from common inputs key goal of neuroscience. In mammalian retina, divergence cone signals into multiple feed-forward bipolar cell pathways forms initial basis for retinal dedicated to specific visual functions. Here, we used patch-clamp electrophysiology, electron microscopy two photon imaging fluorescent glutamate sensor examine kinetically responses arise transient versus sustained ON alpha RGCs (ON-T ON-S RGCs) mouse retina. We directly compared response properties these with their presynaptic partners, which identified using 3D reconstruction. Different subtypes (type 5i, type 6 7) had indistinguishable light-driven whereas extracellular around RGC dendrites postsynaptic excitatory currents measured ON-T identical stimuli probe cells were distinct. Anatomical examination axon terminals suggests subtype-specific differences size synaptic ribbon-associated vesicle pools may contribute kinetics. Our findings indicate synapses are primary point pathways.

Язык: Английский

Процитировано

0