bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 25, 2024

Interneurons (INs) in the mouse olfactory bulb (OB) develop during an extensive period of time that begins embryo and continues throughout lifetime individual. The connectivity location these neurons is profoundly affected by generation, making developmental windows critical to understand circuitry OB. Here, we focus on OB embryonic generated interneurons (or EGINs), which have generally received less attention than those adult. Birthdates EGINs were differentiated injections thymidine analogs their final destinations phenotypes analyzed immunohistochemistry. We found retained adult distributed across all layers However, a lateral-to-medial neurogenic gradient seen only earliest EGINs. Within granule cell layer (GCL), predominantly accumulated superficial region at almost ages remained there adulthood. Immunostaining for calbindin, parvalbumin, tyrosine hydroxylase, calretinin largely negative suggesting may represent subpopulations INs are not yet fully characterized. Using utero electroporation label EGIN progenitors, demonstrated they reach primordial as early E13 begin differentiate apical dendrites E15. At E16, progenitors clustered into chain migrating neuroblasts denoting rostral migratory stream (RMS). Collectively, our data highlight importance studying isolated better formation circuits define responses.

Язык: Английский