The Relationship Between Clinical Features of Ischemic Stroke and miRNA Expression in Stroke Patients: A Systematic Review

Neurology International, Год журнала: 2025, Номер 17(4), С. 55 - 55

Опубликована: Апрель 9, 2025

Background/Objectives: Ischemic stroke remains a leading cause of death and disability worldwide. Despite significant progress in reperfusion therapy, the optimal ischemic management strategy has not been developed. Recent studies demonstrate that microRNA may play an essential role pathophysiology its possible potential to be treatment target point. The proposed systematic review aimed report relationship between IS's clinical severity miRNA expression. Secondary outcomes included infarct volume, systemic inflammatory markers, prognosis, as well additional features such subtype, comorbidity, risk subsequent correlation Methods: We have performed search database resources according PRISMA statement guidelines. Twenty-seven on total number 3906 patients were assessed suitable for present SR. Included analyzed expression 30 different fragments. Results: After investigating available data, we identified set fragment candidates used diagnostics base development future protocols. Conclusions: Studies presented SR indicate significantly associated with severity, inflammation stroke. More prospective, properly designed protocols consistent methods testing optimized assessment are needed confirm course

Язык: Английский

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Alterations in Apoptotic Pathways and Expression of miR-134, miR-181, and miR-497 Induced by Wharton’s Jelly-Derived Mesenchymal Stem Cells in a Rat Model of Ischemic Brain Injury

IBRO Neuroscience Reports, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

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MicroRNA-29a-5p attenuates hemorrhagic transformation and improves outcomes after mechanical reperfusion for acute ischemic stroke

Non-coding RNA Research, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

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MicroRNA Nobel Prize: Timely Recognition and High Anticipation of Future Products—A Prospective Analysis

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12883 - 12883

Опубликована: Ноя. 29, 2024

MicroRNAs (miRNAs) maintain cellular homeostasis by blocking mRNAs binding with them to fine-tune the expression of genes across numerous biological pathways. The 2024 Nobel Prize in Medicine and Physiology for discovering miRNAs was long overdue. We anticipate a deluge research work involving repeat history prizes awarded on other RNAs. Although miRNA therapies are included several complex diseases, realization that regulate their roles addressing hundreds diseases expected; but advancement drug discovery tools, we even faster entry new drugs. To promote this, provide details current science, logic, intellectual property, formulations, regulatory process anticipation many more researchers will introduce novel based discussion advice provided this paper.

Язык: Английский

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Hydrogen sulfide in health and diseases: cross talk with noncoding RNAs

AJP Cell Physiology, Год журнала: 2023, Номер 324(4), С. C856 - C877

Опубликована: Март 7, 2023

Hydrogen sulfide (H2S) is previously described as a potentially lethal toxic gas. However, this gasotransmitter also endogenously generated by the actions of cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (3-MST) in mammalian systems, thus belonging to family gasotransmitters after nitric oxide (NO) carbon monoxide (CO). The physiological or pathological significance H2S has been extensively expanded for decades. Growing evidence revealed that exerts cytoprotective functions cardiovascular, nervous, gastrointestinal systems modulating numerous signaling pathways. With continuous advancement microarray next-generation sequencing technologies, noncoding RNAs (ncRNAs) have gained recognition key players human health diseases due their considerable potential predictive biomarkers therapeutic targets. Coincidentally, ncRNAs are not independent regulators but interact with each other during development progression diseases. Specifically, might serve downstream mediators act on H2S-generating enzymes govern endogenous production. purpose review summarize interactive regulatory roles initiation various explore benefits. This will highlight importance cross talk between disease therapy.

Язык: Английский

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miR-188-5p silencing improves cerebral ischemia/reperfusion injury by targeting Lin28a

Metabolic Brain Disease, Год журнала: 2023, Номер 38(7), С. 2327 - 2338

Опубликована: Авг. 12, 2023

Язык: Английский

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MiR-122 overexpression alleviates oxygen–glucose deprivation-induced neuronal injury by targeting sPLA2-IIA

Frontiers in Neurology, Год журнала: 2024, Номер 15

Опубликована: Май 10, 2024

Background Ischemic stroke (IS) is a neurological disease with significant disability and mortality. MicroRNAs were proven to be associated cerebral ischemia. Previous studies have demonstrated miR-122 downregulation in both animal models of IS the blood patients. Nonetheless, role mechanism miR-122-5p remain unclear. Methods We established primary human mouse astrocytes, along HT22 hippocampal neuronal cells, through oxygen–glucose deprivation/reoxygenation (OGD/R) treatment. To assess impact miR-122, we employed CCK8 assays, flow cytometry, RT-qPCR, western blotting, ELISA evaluate cell viability, apoptosis, reactive oxygen species (ROS) generation, cytokine expression. A dual-luciferase reporter gene assay was investigate interaction between sPLA2-IIA. Results Overexpression resulted decreased reduced cleaved caspase-3 expression, increased viability astrocytes cells subjected OGD/R. RT-qPCR analyses decrease mRNA levels interleukin (IL)-6 tumor necrosis factor (TNF)-α following overexpression. Moreover, overexpression reversed OGD/R-induced ROS 8-OHdG formation astrocytes. Additionally, protein expression inducible nitric oxide synthase (iNOS). Furthermore, found that attaches 3′-UTR sPLA2-IIA, thereby downregulate its Conclusion Our study demonstrates miR-122-mediated inhibition sPLA2-IIA attenuates injury by suppressing alleviating post-ischemic inflammation, reducing production. Thus, miR-122/sPLA2-IIA axis may represent promising target for

Язык: Английский

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miR-135a-5p alleviates cerebral ischemia-reperfusion injury by inhibiting pyroptosis mediated through the DDX3X/NLRP3 pathway

Experimental Neurology, Год журнала: 2024, Номер 385, С. 115127 - 115127

Опубликована: Дек. 22, 2024

Язык: Английский

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MicroRNAs in the Regulation of NADPH Oxidases in Vascular Diabetic and Ischemic Pathologies: A Case for Alternate Inhibitory Strategies?

Antioxidants, Год журнала: 2022, Номер 12(1), С. 70 - 70

Опубликована: Дек. 29, 2022

Since their discovery in the vasculature, different NADPH oxidase (NOX) isoforms have been associated with numerous complex vascular processes such as endothelial dysfunction, inflammation, arterial remodeling, and dyslipidemia. In turn, these often underlie cardiovascular metabolic pathologies including diabetes mellitus type II, cardiomyopathy, systemic pulmonary hypertension atherosclerosis. Increasing attention has directed toward miRNA involvement II its co-morbidities search for predictive stratifying biomarkers therapeutic targets. Owing to challenges of generating isoform-selective NOX inhibitors (NOXi), development specific NOXis suitable purposes hindered. that vein, differential regulation by a particular or combina-tion thereof could at some point become reasonable approach targeting under circumstances. Whereas administration miRNAs chronically, even acutely, patients poses own set difficulties, miRNA-mediated NOXs vasculature is worth surveying. this review, distinct focus on role was made context ischemic injury models.

Язык: Английский

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Effect of dexmedetomidine on tourniquet-induced skeletal muscle injury

Revista da Associação Médica Brasileira, Год журнала: 2023, Номер 69(2), С. 228 - 232

Опубликована: Фев. 1, 2023

The aim of this study was to investigate whether dexmedetomidine could reduce tourniquet-induced skeletal muscle injury.C57BL6 male mice were randomly assigned sham, ischemia/reperfusion, and groups. Mice in the ischemia/reperfusion groups received normal saline solution intraperitoneally, respectively. sham group underwent same procedure as group, with exception tourniquet application. Subsequently, ultrastructure gastrocnemius observed, its contractile force examined. In addition, Toll-like receptor 4 nuclear factor-κB expression within muscles detected by Western blot.Dexmedetomidine alleviated myocyte damage increased contractility muscles. Moreover, significantly inhibited 4/nuclear muscle.Taken together, these results demonstrate that administration attenuated structural functional impairment muscle, partly through inactivation pathway.

Язык: Английский

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