The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo
Nature Methods,
Год журнала:
2023,
Номер
21(1), С. 132 - 141
Опубликована: Дек. 21, 2023
Abstract
Multiphoton
microscopy
can
resolve
fluorescent
structures
and
dynamics
deep
in
scattering
tissue
has
transformed
neural
imaging,
but
applying
this
technique
vivo
be
limited
by
the
mechanical
optical
constraints
of
conventional
objectives.
Short
working
distance
objectives
collide
with
compact
surgical
windows
or
other
instrumentation
preclude
imaging.
Here
we
present
an
ultra-long
(20
mm)
air
objective
called
Cousa
objective.
It
is
optimized
for
performance
across
multiphoton
imaging
wavelengths,
offers
a
more
than
4
mm
2
field
view
submicrometer
lateral
resolution
compatible
commonly
used
systems.
A
novel
design,
wider
typical
microscope
objectives,
enabled
combination
specifications.
We
share
full
prescription,
report
including
two-photon
three-photon
array
species
preparations,
nonhuman
primates.
The
enable
range
experiments
neuroscience
beyond.
Язык: Английский
Wide-field imaging in behaving mice as a tool to study cognitive function
Neurophotonics,
Год журнала:
2024,
Номер
11(03)
Опубликована: Фев. 19, 2024
Cognitive
functions
are
mediated
through
coordinated
and
dynamic
neuronal
responses
that
involve
many
different
areas
across
the
brain.
Therefore,
it
is
of
high
interest
to
simultaneously
record
activity
from
as
brain
possible
while
subject
performs
a
cognitive
behavioral
task.
One
emerging
tools
achieve
mesoscopic
field
view
wide-field
imaging
cortex-wide
dynamics
in
mice.
Wide-field
cost-effective,
user-friendly,
enables
obtaining
signals
mice
performing
complex
demanding
tasks.
Importantly,
offers
an
unbiased
observation
sheds
light
on
overlooked
cortical
regions
highlights
parallel
processing
circuits.
Recent
studies
have
shown
multi-area
patterns,
rather
than
just
single
area,
involved
encoding
functions.
The
optical
properties
enable
signals,
such
layer-specific,
inhibitory
subtypes,
or
neuromodulation
signals.
Here,
I
review
main
advantages
mice,
recent
literature,
discuss
future
directions
field.
It
expected
behaving
will
continue
gain
popularity
aid
understanding
mesoscale
underlying
function.
Язык: Английский
The Cousa objective: a long working distance air objective for multiphoton imagingin vivo
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2022,
Номер
unknown
Опубликована: Ноя. 6, 2022
Abstract
Multiphoton
microscopy
can
resolve
fluorescent
structures
and
dynamics
deep
in
scattering
tissue,
but
applying
this
technique
vivo
be
limited
by
short
working
distance
water-immersion
objectives.
Here
we
present
an
ultra
long
(20
mm)
air
objective
called
the
Cousa
objective.
It
is
optimized
for
performance
across
multiphoton
imaging
wavelengths,
offers
a
>
4
mm
2
field-of-view
with
submicron
lateral
resolution,
compatible
commonly
used
systems.
We
share
full
optical
prescription,
report
including
2-photon
3-photon
range
of
species
preparations.
Язык: Английский