The Cousa objective: a long working distance air objective for multiphoton imagingin vivo DOI Creative Commons
Che‐Hang Yu, Yiyi Yu,

Liam M. Adsit

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Ноя. 6, 2022

Abstract Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue, but applying this technique vivo be limited by short working distance water-immersion objectives. Here we present an ultra long (20 mm) air objective called the Cousa objective. It is optimized for performance across multiphoton imaging wavelengths, offers a > 4 mm 2 field-of-view with submicron lateral resolution, compatible commonly used systems. We share full optical prescription, report including 2-photon 3-photon range of species preparations.

Язык: Английский

The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo DOI Creative Commons
Che‐Hang Yu, Yiyi Yu,

Liam M. Adsit

и другие.

Nature Methods, Год журнала: 2023, Номер 21(1), С. 132 - 141

Опубликована: Дек. 21, 2023

Abstract Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue has transformed neural imaging, but applying this technique vivo be limited by the mechanical optical constraints of conventional objectives. Short working distance objectives collide with compact surgical windows or other instrumentation preclude imaging. Here we present an ultra-long (20 mm) air objective called Cousa objective. It is optimized for performance across multiphoton imaging wavelengths, offers a more than 4 mm 2 field view submicrometer lateral resolution compatible commonly used systems. A novel design, wider typical microscope objectives, enabled combination specifications. We share full prescription, report including two-photon three-photon array species preparations, nonhuman primates. The enable range experiments neuroscience beyond.

Язык: Английский

Процитировано

25

Wide-field imaging in behaving mice as a tool to study cognitive function DOI Creative Commons
Ariel Gilad

Neurophotonics, Год журнала: 2024, Номер 11(03)

Опубликована: Фев. 19, 2024

Cognitive functions are mediated through coordinated and dynamic neuronal responses that involve many different areas across the brain. Therefore, it is of high interest to simultaneously record activity from as brain possible while subject performs a cognitive behavioral task. One emerging tools achieve mesoscopic field view wide-field imaging cortex-wide dynamics in mice. Wide-field cost-effective, user-friendly, enables obtaining signals mice performing complex demanding tasks. Importantly, offers an unbiased observation sheds light on overlooked cortical regions highlights parallel processing circuits. Recent studies have shown multi-area patterns, rather than just single area, involved encoding functions. The optical properties enable signals, such layer-specific, inhibitory subtypes, or neuromodulation signals. Here, I review main advantages mice, recent literature, discuss future directions field. It expected behaving will continue gain popularity aid understanding mesoscale underlying function.

Язык: Английский

Процитировано

2

The Cousa objective: a long working distance air objective for multiphoton imagingin vivo DOI Creative Commons
Che‐Hang Yu, Yiyi Yu,

Liam M. Adsit

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Ноя. 6, 2022

Abstract Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue, but applying this technique vivo be limited by short working distance water-immersion objectives. Here we present an ultra long (20 mm) air objective called the Cousa objective. It is optimized for performance across multiphoton imaging wavelengths, offers a > 4 mm 2 field-of-view with submicron lateral resolution, compatible commonly used systems. We share full optical prescription, report including 2-photon 3-photon range of species preparations.

Язык: Английский

Процитировано

5