Cortical Response to Acute Implantation of the Utah Optrode Array in Macaque Cortex DOI Open Access

Adrián Villamarin‐Ortiz,

Christopher F. Reiche, Frederick Federer

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Optogenetics has transformed the study of neural circuit function, but limitations in its application to species with large brains, such as non-human primates (NHPs), remain. A major challenge NHP optogenetics is delivering light sufficiently volumes deep tissue high spatiotemporal precision, without simultaneously affecting superficial tissue. To overcome these limitations, we recently developed and tested vivo cortex, Utah Optrode Array (UOA). This a 10×10 array penetrating glass shanks, tiling 4×4mm 2 area, bonded interleaved needle-aligned interstitial µLED arrays, which allows for independent photostimulation brain Here, investigate acute biological response UOA implantation goal optimizing device design reduced insertion trauma subsequent chronic response. this goal, systematically vary shank diameter, surface texture, tip geometry, pressure, assess their effects on astrocytes, microglia, neuronal viability, following implantation. We find that UOAs shanks smaller smooth texture round tips cause least damage. Higher pressures have limited inflammatory response, lead greater compression. Our results highlight importance balancing pressure preserving integrity improving long-term performance biocompatibility.

Язык: Английский

Minimally Invasive Bladder Stimulation via Upconversion Nanoparticle-Mediated Optogenetics DOI
Zhonghan Zhou, Xuesheng Wang, Limin Liao

и другие.

ACS Applied Nano Materials, Год журнала: 2024, Номер 7(5), С. 5652 - 5660

Опубликована: Фев. 19, 2024

Currently, there are no clinically proven alternative treatments for inducing or enhancing bladder contractions. In this study, we applied optogenetics to modulate function, successfully contractions with 475 nm blue light after transfecting channelrhodopsin-2 (ChR2) into smooth muscle cells. However, it requires laparotomy and exposure due restricted tissue penetration of visible light. Hence, developed a minimally invasive approach utilizing penetrating near-infrared (NIR) via upconversion nanoparticles (UCNPs) as an intermediary control. The core–shell UCNPs NaYF4:Yb/Tm@PEG were designed. Excited at 980 nm, emitted predominantly matching the excitation wavelength ChR2. We reasoned feasibility UCNPs-mediated system its efficacy safety. our experiment, confirmed that by under NIR is sufficient activate ChR2 induce cation influx in Moreover, has low cell toxicity acceptable photothermal effects. No significant UNCPs was detected. Then, performed ex vivo pressure recording cystometry 4 weeks injection transfection. An 8.0 W transdermal illumination delivered from optical laser, need procedures expose bladder. result showed induced ChR2-transfected intermediation UCNPs. Compared spontaneous voiding, NIR-induced voiding exhibited higher maximum detrusor micturition volume. These findings demonstrated can serve

Язык: Английский

Процитировано

3
Cortical Response to Acute Implantation of the Utah Optrode Array in Macaque Cortex DOI Open Access

Adrián Villamarin‐Ortiz,

Christopher F. Reiche, Frederick Federer

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Optogenetics has transformed the study of neural circuit function, but limitations in its application to species with large brains, such as non-human primates (NHPs), remain. A major challenge NHP optogenetics is delivering light sufficiently volumes deep tissue high spatiotemporal precision, without simultaneously affecting superficial tissue. To overcome these limitations, we recently developed and tested vivo cortex, Utah Optrode Array (UOA). This a 10×10 array penetrating glass shanks, tiling 4×4mm 2 area, bonded interleaved needle-aligned interstitial µLED arrays, which allows for independent photostimulation brain Here, investigate acute biological response UOA implantation goal optimizing device design reduced insertion trauma subsequent chronic response. this goal, systematically vary shank diameter, surface texture, tip geometry, pressure, assess their effects on astrocytes, microglia, neuronal viability, following implantation. We find that UOAs shanks smaller smooth texture round tips cause least damage. Higher pressures have limited inflammatory response, lead greater compression. Our results highlight importance balancing pressure preserving integrity improving long-term performance biocompatibility.

Язык: Английский

Процитировано

0