Imaging the enteric nervous system
Doriane Hazart,
Marwa Moulzir,
Brigitte Delhomme
и другие.
Frontiers in Neuroanatomy,
Год журнала:
2025,
Номер
19
Опубликована: Март 12, 2025
The
enteric
nervous
system
(ENS)
has
garnered
increasing
scientific
interest
due
to
its
pivotal
role
in
digestive
processes
and
involvement
various
gastrointestinal
central
(CNS)
disorders,
including
Crohn’s
disease,
Parkinson’s
autism.
Despite
significance,
the
ENS
remains
relatively
underexplored
by
neurobiologists,
primarily
because
structure
function
are
less
understood
compared
CNS.
This
review
examines
both
pioneering
methodologies
that
initially
revealed
intricate
layered
of
recent
advancements
studying
three-dimensional
(3-D)
organization,
fixed
samples
at
a
functional
level,
ex-vivo
or
in-vivo
.
Traditionally,
imaging
relied
on
histological
techniques
involving
sequential
tissue
sectioning,
staining,
microscopic
single
sections.
However,
this
method
limitations
representing
full
complexity
ENS’s
3-D
meshwork,
which
led
development
more
intact
preparations,
such
as
whole-mount
preparation,
well
use
volume
techniques.
Advancements
imaging,
particularly
methods
like
spinning-disk
confocal,
2-photon,
light-sheet
microscopies,
combined
with
tissue-clearing
techniques,
have
revolutionized
our
understanding
fine
structure.
These
approaches
offer
detailed
views
cellular
architecture,
interactions
among
cell
types,
blood
vessels,
lymphatic
vessels.
They
also
enhanced
comprehension
ENS-related
pathologies,
inflammatory
bowel
Hirschsprung’s
disease
(HSCR),
neurodegenerative
disorders
(PD)
Alzheimer’s
diseases
(AD).
More
recently,
2-photon
confocal
transgenic
for
calcium
laser
endomicroscopy,
opened
new
avenues
studies
ENS.
enable
real-time
observation
neuronal
glial
activity
their
interactions.
While
routinely
used
CNS
studies,
application
local
circuits
signals
is
presents
unique
challenges,
accommodating
peristaltic
movements.
expected
significantly
deepen
roles
neurological
diseases,
potentially
leading
improved
diagnostic
therapeutic
strategies.
Язык: Английский
Microfabrication Technologies for Nanoinvasive and High‐Resolution Magnetic Neuromodulation
Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 24, 2024
Abstract
The
increasing
demand
for
precise
neuromodulation
necessitates
advancements
in
techniques
to
achieve
higher
spatial
resolution.
Magnetic
stimulation,
offering
low
signal
attenuation
and
minimal
tissue
damage,
plays
a
significant
role
neuromodulation.
Conventional
transcranial
magnetic
stimulation
(TMS),
though
noninvasive,
lacks
the
resolution
neuron
selectivity
required
spatially
To
address
these
limitations,
next
generation
of
neurostimulation
technologies
aims
submillimeter‐resolution
selective
with
high
temporal
Invasive
nanoinvasive
are
two
next‐generation
approaches:
invasive
methods
use
implantable
microcoils,
while
nanoparticles
(MNPs)
This
review
will
introduce
working
principles,
technical
details,
coil
designs,
potential
future
developments
approaches
from
an
engineering
perspective.
Furthermore,
discuss
state‐of‐the‐art
microfabrication
depth
due
its
irreplaceable
realizing
In
addition
reviewing
neuromodulation,
this
cover
through‐silicon
vias
(TSV),
surface
micromachining,
photolithography,
direct
writing,
other
fabrication
technologies,
supported
by
case
studies,
providing
framework
integration
microelectronics
technologies.
Язык: Английский
Electromagnetic Radiation and Biophoton Emission in Neuronal Communication and Neurodegenerative Diseases
Progress in Biophysics and Molecular Biology,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 1, 2024
Язык: Английский
Exploring the Interface of Microwave Technology, Quantum Computing and Neuroscience
Опубликована: Июнь 14, 2024
Microwave
technology,
foundational
in
quantum
computing,
utilizes
precise
control
of
states
via
microwave
pulses
to
manage
bits
(qubits).
This
review
explores
the
potential
leveraging
these
principles
neuroscience,
where
such
technology
could
enable
novel
approaches
imaging,
diagnosing,
and
treating
neurological
disorders.
By
comparing
techniques
used
computing
with
applications
neural
circuit
manipulation
brain
activity
monitoring,
we
highlight
both
promising
synergies
significant
challenges
at
intersection
fields.
interdisciplinary
exploration
not
only
underscores
transformative
neuroscience
but
also
addresses
ethical
considerations
technological
hurdles
that
accompany
integration
advanced
mechanics
into
biological
contexts.
The
convergence
science
opens
a
pathway
for
breakthroughs
understanding
complex
disorders,
advocating
cautious
yet
optimistic
approach
towards
future
research
application.
Язык: Английский
Brain-implantable needle-type CMOS imaging device enables multi-layer dissection of seizure calcium dynamics in the hippocampus
Journal of Neural Engineering,
Год журнала:
2024,
Номер
21(4), С. 046022 - 046022
Опубликована: Июнь 26, 2024
:
Current
neuronal
imaging
methods
mostly
use
bulky
lenses
that
either
impede
animal
behavior
or
prohibit
multi-depth
imaging.
To
overcome
these
limitations,
we
developed
a
lightweight
lensless
biophotonic
system
for
imaging,
enabling
compact
and
simultaneous
visualization
of
multiple
brain
layers.
Язык: Английский
Low-NA Two-photon lithography patterning of metal/dielectric tapered optical fibers for depth-selective, volumetric optical neural interfaces
Optics Express,
Год журнала:
2024,
Номер
32(27), С. 48772 - 48772
Опубликована: Ноя. 6, 2024
Optical
neural
implants
allow
neuroscientists
to
access
deep
brain
regions,
enabling
decipher
complex
patterns
of
activity.
In
this
field,
the
use
optical
fibers
is
rapidly
increasing,
and
ability
generate
high-quality
metal
on
their
non-planar
surface
would
further
extend
application.
Here,
we
propose
alternating
shielding
dielectric
confinement
engineer
mode-division
properties
tapered
fiber
implants.
This
accomplished
through
an
unconventional
application
two-photon
lithography
(TPL),
which
employs
a
low-numerical
aperture
objective
pattern
extensive
waveguide
sections
at
both
low
high
curvature
radii.
The
low-NA
TPL
used
polymerize
mask
photoresist,
while
rest
taper
undergoes
wet
etching.
implies
no
direct
destructive
interaction
between
laser
beam
be
removed,
preserving
coating.
advantages
provided
by
presented
fabrication
method,
combined
with
intrinsic
modal
waveguide,
enable
engineering
light
guiding
mechanisms,
achieving
depth-selective
delivery
extinction
ratio.
device's
emission
collection
were
investigated
in
quasi-transparent
media
highly
scattering
slices,
finding
that
our
proposed
method
facilitates
360°
symmetric
around
dielectric-confined
section
depth
resolution.
opens
perspective
for
realization
can
interface
implant
axis
all-around,
also
applied
other
types
surfaces.
Язык: Английский
Small form factor implantable neural probe with efficient flip chip micro LED for in vivo optogenetics
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 18, 2024
Abstract
Optogenetics
is
a
widely
used
tool
to
dissect
neural
circuits
with
optical
stimulation,
but
it
requires
that
light
delivered
photosensitive
neurons
inside
the
brain.
Implantable
probes
microscale
LEDs
(μLEDs)
are
an
emerging
approach
delivering
brain
superior
output
control.
However,
approaches
integrate
μLEDs
in
depend
on
complex
fabrication
processes.
Here,
we
developed
implantable
small
form
factor
probe
integrates
highly
efficient
commercial
flip
chip
using
only
standard
lithography
processes
silicon
and
custom
automated
LED
mounting
3D-printed
tools
pick-and-place
machine.
The
has
cross-sectional
area
under
0.013
mm
2
can
up
2.3
mW
of
power
irradiance
175
mW/mm
.
Due
high
plug
efficiency
wide
large
interconnect
lines,
improved
heat
dissipation
properties
perform
stimulation
protocols
50
Hz
90%
duty
cycles
without
surpassing
hotspot
temperature
elevations
above
1
ºC.
were
validated
vivo,
activity
motor
cortex
transgenic
optogenetic
mice
being
reliably
modulated
by
pulsed
emitted
from
probe.
Язык: Английский