Effect of Isoflavone on Muscle Atrophy in Ovariectomized Mice DOI Open Access

S Kawai,

Takuro Okamura, Chihiro Munekawa

и другие.

Nutrients, Год журнала: 2024, Номер 16(19), С. 3295 - 3295

Опубликована: Сен. 28, 2024

Background: Sarcopenia, characterized by muscle mass decline due to aging or other causes, is exacerbated decreased estrogen levels after menopause in women. Isoflavones, a class of flavonoids acting on receptors, may have beneficial effects metabolic disorders. We examined these ovariectomized mice fed high-fat, high-sucrose diet (HFHSD). Methods: At 7 weeks old, female C57BL6/J (18–20 g, n = 12) underwent bilateral ovariectomy (OVX), and were then starting at 8 age. Half the received isoflavone water (0.1%). Metabolic analyses, including glucose insulin tolerance tests, conducted. Muscle analysis involved grip strength assays, next-generation sequencing, quantitative RT–PCR, western blotting skeletal euthanizing 14 old. Additionally, 16S rRNA gene sequence gut microbiota was performed. Results: The results demonstrated that administration did not affect body weight, tolerance, lipid metabolism. In contrast, isoflavone-treated had higher strength. Gene expression soleus revealed Trim63 expression, showed inactivation muscle-specific RING finger protein 1 mice. Gut indicated Bacteroidetes lower Firmicutes abundance group, along with increased diversity. sets related TNF-α signaling via NF-κB unfolded response negatively associated isoflavones. Conclusions: Isoflavone intake alters increases strength, suggesting potential role improving sarcopenia menopausal

Язык: Английский

Emerging insights into the gut microbiota as a key regulator of immunity and response to immunotherapy in hepatocellular carcinoma DOI Creative Commons
Siqi Ren, Yinping Zhang,

Xingyue Wang

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Фев. 25, 2025

The gut microbiota, a complex microbial ecosystem closely connected to the liver via portal vein, has emerged as critical regulator of health and disease. Numerous studies have underscored its role in onset progression disorders, including alcoholic disease, metabolic dysfunction-associated steatotic disease (MASLD), steatohepatitis (MASH), fibrosis, cirrhosis, hepatocellular carcinoma (HCC). This review provides comprehensive overview current insights into influence microbiota on HCC progression, particularly effects immune cells within tumor microenvironment (TME). Furthermore, we explore potential microbiota-targeted interventions, such antibiotics, probiotics, prebiotics, fecal transplantation (FMT), modulate response improve outcomes immunotherapy HCC. By synthesizing from recent studies, this aims highlight microbiota-based strategies that may enhance outcomes, advancing personalized approaches treatment.

Язык: Английский

Процитировано

0

Heat exposure promotes sarcopenia via gut microbiota‐derived metabolites DOI Creative Commons

Yi‐Fan Guo,

Zheyu Liu, Min Zhou

и другие.

Aging Cell, Год журнала: 2024, Номер unknown

Опубликована: Окт. 29, 2024

Abstract The unprecedented rise in global ambient temperatures the last decade has significantly impacted human health, yet how heat exposure affects development of sarcopenia remains enigmatic. Here, we demonstrate that chronic induces skeletal muscle volume loss, leading to strength and functional decline mice. microbiota composition heat‐exposed mice was analyzed using 16S ribosomal DNA analysis. Liquid chromatography‐mass spectrometry (LC–MS) used explore effects on blood metabolome further analyze correlation between metabolism gut microbiota. Transplantation from germ‐free sufficient increase adverse function host. Mechanistically, an untargeted metabolomics strategy, reveal altered due high is associated with elevated serum levels homocitrulline. Homocitrulline causes mitochondrial dysfunction myocytes by exacerbating ferroptosis levels. And Nrf2 activator (Oltipraz) supplementation alleviates atrophy induced exposure. Our findings detrimental provide new strategies for treating sarcopenia.

Язык: Английский

Процитировано

2

The Relationship Between Gut Microbiota, Muscle Mass and Physical Function in Older Individuals: A Systematic Review DOI Open Access
David Barry, Sam Wu, Matthew B. Cooke

и другие.

Nutrients, Год журнала: 2024, Номер 17(1), С. 81 - 81

Опубликована: Дек. 28, 2024

Background: Recent evidence suggests that sarcopenia and subsequent changes in muscle mass functional outcomes are linked to disruption the gastrointestinal microbiota composition and/or function via microbiota-gut-muscle axis. Despite growing interest, few studies have systemically analysed (1) relationship between gut microbiota, physical performance (2) effects of gut-modulating dietary interventions on these within older individuals with or without sarcopenia. Methods: Four electronic databases (PubMed, MEDLINE, Embase Scopus) were searched for articles published from year 2004 until July 2023. The Preferred Reporting Items Systematic Reviews Meta-Analyses (PRISMA) followed. Revised Cochrane Risk Bias (RoB 2.0) Joanna Briggs Institute (JBI) critical appraisal checklist utilised evaluate risk bias intervention observational studies, respectively. Results: A total 20 (14 6 interventional) involving 4071 participants (mean age 69.9 years, 51.6% female) included. There was significant heterogeneity regarding outcome measures used studies. Correlations diversity sarcopenia-related observed. Interventional targeting resulted improved strength, body some, but not all, Conclusions: limitations reviewed, findings provide further development is likely influenced by an altered microbial environment microbiome could hold therapeutic potential treatment management

Язык: Английский

Процитировано

2

Effect of Isoflavone on Muscle Atrophy in Ovariectomized Mice DOI Open Access

S Kawai,

Takuro Okamura, Chihiro Munekawa

и другие.

Nutrients, Год журнала: 2024, Номер 16(19), С. 3295 - 3295

Опубликована: Сен. 28, 2024

Background: Sarcopenia, characterized by muscle mass decline due to aging or other causes, is exacerbated decreased estrogen levels after menopause in women. Isoflavones, a class of flavonoids acting on receptors, may have beneficial effects metabolic disorders. We examined these ovariectomized mice fed high-fat, high-sucrose diet (HFHSD). Methods: At 7 weeks old, female C57BL6/J (18–20 g, n = 12) underwent bilateral ovariectomy (OVX), and were then starting at 8 age. Half the received isoflavone water (0.1%). Metabolic analyses, including glucose insulin tolerance tests, conducted. Muscle analysis involved grip strength assays, next-generation sequencing, quantitative RT–PCR, western blotting skeletal euthanizing 14 old. Additionally, 16S rRNA gene sequence gut microbiota was performed. Results: The results demonstrated that administration did not affect body weight, tolerance, lipid metabolism. In contrast, isoflavone-treated had higher strength. Gene expression soleus revealed Trim63 expression, showed inactivation muscle-specific RING finger protein 1 mice. Gut indicated Bacteroidetes lower Firmicutes abundance group, along with increased diversity. sets related TNF-α signaling via NF-κB unfolded response negatively associated isoflavones. Conclusions: Isoflavone intake alters increases strength, suggesting potential role improving sarcopenia menopausal

Язык: Английский

Процитировано

0