Structural characterization and antioxidant activity of a polysaccharide produced by Chaetomium globosum CGMCC 6882 from waste tobacco leaves

Preparative Biochemistry & Biotechnology, Год журнала: 2025, Номер unknown, С. 1 - 10

Опубликована: Фев. 18, 2025

Due to the restricts of treatment methods and high costs, waste tobacco materials are usually discarded, which leads resources environmental pollution. Present work used leaves as carbon source produce polysaccharide (CGP-TL) by Chaetomium globosum CGMCC 6882 via submerged fermentation. CGP-TL yield was 1.62 ± 0.35 g/L, contents carbohydrate protein in were 91.73% 6.49% 5.51% 0.84%. Structural characterization showed composed rhamnose, arabinose, galactose, glucose, mannose xylose a molar ratio 9.9688: 6.8482: 19.6045: 29.4448: 0.8033: 0.5874. Meanwhile, its weight-average molecular weight, number-average weight polydispersity 286.035 kDa, 118.146 kDa 2.421, respectively. In vitro antioxidant analysis scavenged DPPH, ABTS, hydroxyl superoxide radicals positively correlated concentration, corresponding scavenging effects at 2.5 mg/mL 75.53% 1.89%, 96.24% 2.32%, 52.91% 2.28% 65.26% 2.47%. Furthermore, not only improved antioxidative status RAW 264.7 cells, but also exerted oxidative repairing on H2O2-damaged cells increasing levels SOD, CAT GSH-Px, reducing MDA level. Overall, this will provide references for green efficient utilization cellulose resources.

Язык: Английский

Alleviating effect of Lactobacillus fermentum E15 on hyperlipidemia and hepatic lipid metabolism in zebrafish fed by a high-fat diet through the production of short-chain fatty acids

Frontiers in Nutrition, Год журнала: 2025, Номер 12

Опубликована: Март 3, 2025

Introduction Hyperlipidemia is regarded as one of the crucial factors leading to atherosclerosis and other cardiovascular diseases. Gut microbiota plays an important role in regulating host lipid metabolism. Nevertheless, exact mechanisms behind this remain unclear. Methods In present study, a hyperlipidemic zebrafish model was established using high-cholesterol diet (HCD) evaluate anti-hyperlipidemic effects Lactobacillus fermentum E15 ( L. E15). Results showed that effectively reduced accumulation blood vessels liver HCD-fed larvae. Meanwhile, improved abnormal levels, normalized enzyme activity. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis revealed downregulated expression sterol regulatory element-binding factor (SREBP-1), peroxisome proliferator-activated receptor-gamma (PPAR- γ ), fatty acid synthase (Fasn), while upregulated receptor-alpha α ). Additionally, metabolomic produced series short-chain acids (SCFAs), including acetic acid, propionic butyric isovaleric acid. Notably, contributed reduction droplet contrast, blocking G-protein coupled receptor 43 (GPR43) with pertussis toxin (PTX) abolished on reducing RT-qPCR results further suggested both promoted GPR43 leptin A, which inhibited by PTX. Conclusion These findings alleviates HCD-induced hyperlipidemia activating through SCFAs.

Язык: Английский

Insight into the relationship between structure and immunomodulatory activity of enzymatic degradated Dendrobium officinale polysaccharide fractions

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106623 - 106623

Опубликована: Апрель 1, 2025

Язык: Английский