Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 3, 2023
Abstract
Colorectal
cancer
(CRC)
is
deadly
anaplastic
changes
in
the
gastrointestinal
tract
with
high-rate
mortality.
In
recent
years,
application
of
phytocompounds
has
been
extended
along
different
therapeutic
protocols.
Here,
we
monitored
effects
Thymoquinone
(TQ)
on
autophagy
via
mitochondrial
function
after
modulation
Wnt/β-catenin
signaling
pathway.
Human
colorectal
adenocarcinoma
HT-29
cells
were
treated
TQ
(60
μM)
and
Wnt3a
inhibitor
(LGK974;
15
for
48
hours.
The
survival
rate
was
evaluated
using
an
MTT
assay.
expression
Wnt-related
factors
(c-Myc,
Axin),
angiogenesis
(VE-Cadherin),
mitophagy-related
(PINK1,
OPTN)
assessed
real-time
PCR
Protein
levels
(Beclin-1,
LC3,
P62)
western
blotting.
Using
flow
cytometry
analysis,
intracellular
accumulation
Rhodamine
123
evaluated.
migration
properties
analyzed
a
scratch
wound
healing
Data
indicated
that
can
reduce
viability
compared
to
control
(p<0.05).
VE-Cadherin
inhibited
while
PINK1
induced
Both
LGK974
TQ-treated
exhibited
activation
flux
(Beclin-1↑,
LC3II/I↑,
p62↓)
group
increase
123,
indicating
inhibition
efflux
mechanisms
cells.
Along
these
changes,
also
reduced.
potential
alter
dynamic
growth
human
autophagy,
mechanisms.
Drugs and Drug Candidates,
Год журнала:
2023,
Номер
2(2), С. 516 - 537
Опубликована: Июнь 17, 2023
Inflammation
is
an
essential
defense
mechanism
against
harmful
stimuli.
However,
uncontrolled
inflammatory
mechanisms
culminate
in
disturbed
responses
that
contribute
to
multiple
serious
diseases.
Besides
common
synthetic
drugs,
there
a
growing
interest
optimizing
the
use
of
natural
products
as
therapeutic
or
protective
supplements
disorders.
Black
cumin
seed
(BCS),
Nigella
sativa
(Family
Ranunculaceae),
widely
used
health-supportive
herb
Middle
East,
Far
East
and
West
Asia.
BCS
rich
source
phytochemicals,
studies
have
reported
its
promising
effects
variety
metabolic,
proliferative,
respiratory,
neurological
disorders
associated
with
disrupted
pathways.
This
review
presents
updated
comprehensive
assessment
BCS’s
various
highlights
role
bioactive
constituents
inflammation
oxidative
stress
Moreover,
it
outlines
future
possibilities
for
enhancing
activity
through
efficient
pharmaceutical
formulations.
Thorough
analysis
international
research
published
between
years
1998
2023
reveals
anti-inflammatory
potential
modulating
crucial
players
Thus,
can
be
further
boosted
by
technologies
such
nano-incorporation
improved
management
Current Issues in Molecular Biology,
Год журнала:
2023,
Номер
45(4), С. 3016 - 3034
Опубликована: Апрель 3, 2023
Nigella
sativa
L.
(family
Ranunculaceae),
also
known
as
black
cumin,
has
been
used
in
cuisine
around
the
world
for
many
years.
Due
to
its
health-promoting
properties,
it
can
be
not
only
food
industry
but
medicine.
The
main
bioactive
compound
contained
cumin
extract
is
thymoquinone
(TQ),
which
a
special
therapeutic
role.
results
of
research
recent
years
confirmed
hypoglycemic,
hypolipemic,
and
hepatoprotective
effects,
among
others.
In
addition,
laboratory
tests
indicate
immunomodulatory
anticancer
although
there
still
lack
data
on
mechanisms
how
they
are
involved
fight
against
cancer.
Including
this
plant
material
one’s
diet
both
an
element
prophylaxis
therapy
supporting
treatment
process,
including
pharmacological
treatment.
However,
attention
should
paid
potential
interactions
with
drugs
chronic
diseases.
black
seed
(Nigella
sativa
L.)
has
long
been
utilized
in
traditional
medicine
and
as
a
food
ingredient
due
to
its
potential
therapeutic
properties
including
effectiveness
against
cancer,
coronaviruses,
bacterial
infections.
Recently,
it
garnered
significant
attention
for
rich
reservoir
of
beneficial
secondary
metabolites.
In
vitro
culture
seeds
presents
an
efficient
modern
approach
the
large-scale
production
these
valuable
compounds,
offering
advantages
such
space
efficiency,
reduced
time,
lower
costs.
This
study
aimed
develop
optimize
protocol
callus
induction
identification
key
metabolites,
thymoquinone
(TQ),
phenolic
flavonoids.
To
induce
formation
explants,
two
plant
growth
regulators
(PGRs)
were
applied
individually
or
combination
incorporated
into
Murashige
Skoog
(MS)
medium.
The
Auxin,
2,4-dichlorophenoxyacetic
acid
(2,4-D)
cytokinin,
6-benzylaminopurine
(BAP),
effectively
induced
most
with
response
varying
based
on
concentration.
highest
fresh
weight
(7.02
g)
was
obtained
Red(R)
LED
lighting
FeO3-CTs
nanoparticles
(100
mg/L-
1),
which
also
resulted
dry
(1.307
after
40
days
cultivation.
Similarly,
levels
phenols,
flavonoids
amino
acids
observed
under
R
mg
L-
while
at
100
200
1)
exhibited
effects
metabolite
production.
contrast,
antioxidant
activity
DPPH
free
radicals
total
carbohydrate
accumulation
enhanced
cultures
treated
(200
dark
conditions.
Additionally,
GC-MS
analysis
revealed
that
yielded
effective
enhancement
metabolites
blue
(B)
light
concentration
295
1.
finding
this
highlights
proposed
method
carbohydrates,
acids,
from
controlled
environment.
optimized
offers
cost-effective
space-efficient
strategy
enhancing
bioactive
compound
synthesis,
applications
pharmaceutical
nutraceutical
industries.
Heliyon,
Год журнала:
2024,
Номер
10(2), С. e24840 - e24840
Опубликована: Янв. 1, 2024
PurposeThe
aim
of
the
study
was
to
compare
ameliorating
effects
thymoquinone
at
various
dosages
on
cisplatin-induced
renal
toxicity,
and
investigate
its
nephrocyte
apoptosis
via
mitochondrial
pathway
in
a
rat
model.MethodsA
model
damage
established,
with
treatment
groups
(receiving
1,
3,
5,
10,
or
20
mg/kg
thymoquinone).
We
determined
serum
creatinine
(Cr)
blood
urea
nitrogen
(BUN),
measured
expression
anti-apoptotic
protein
Bcl-2,
pro-apoptotic
Bax,
caspase-3,
kidney
injury
molecule-1
(KIM-1)
neutrophil
gelatinase-associated
lipocalin
(NGAL)
tissue.
Additionally,
we
observed
pathological
changes
tissue
performed
paller
score
for
tubule
injury.ResultsRelative
control,
cisplatin
group
exhibited
significantly
elevated
NGAL
KIM-1
expression,
Cr
BUN
concentrations
reduced
Bcl-2
(P
<
0.05).
Histopathological
examination
cisplatin-treated
revealed
vacuolar
degeneration,
tubular
epithelial
cell
swelling,
an
absence
brush
margins
tubules.
Paller
relative
normal
control
group.
Thymoquinone
dose-dependently
ameliorated
these
effects.ConclusionThymoquinone
1–20
improved
dysfunction
rats.
This
protective
effect
is
related
inhibition
mitochondria-mediated
apoptosis.
Applied Sciences,
Год журнала:
2024,
Номер
14(19), С. 8843 - 8843
Опубликована: Окт. 1, 2024
Oilseed
plants
are
a
valuable
group
of
foods
in
human
nutrition.
Uncommon
oilseed
plants,
such
as
safflower
(Carthamus
tinctorius
L.),
camelina
(Camelina
sativa
and
black
cumin
(Nigella
have
been
gaining
increasing
attention.
The
unique
chemical
compositions
these
which
contribute
to
potential
health
benefits,
underscore
the
importance
further
exploration
study.
continuation
development
research
this
field,
resulting
well-documented
positive
impact
on
health,
may
lead
possibility
them
becoming
new
functional
future.
This
review
aims
present
health-promoting
properties
aforementioned
uncommon
based
recent
literature
reports
intends
inspire
their
features.
Among
others,
anticancer,
antioxidant,
antibacterial
potential,
well
preventive
case
metabolic
diseases
were
discussed.
problems
was
also
emphasized.
BMC Complementary Medicine and Therapies,
Год журнала:
2024,
Номер
24(1)
Опубликована: Март 26, 2024
Abstract
Colorectal
cancer
(CRC)
is
deadly
anaplastic
changes
in
the
gastrointestinal
tract
with
high-rate
mortality.
In
recent
years,
application
of
phytocompounds
has
been
extended
along
different
therapeutic
protocols.
Here,
we
monitored
effects
Thymoquinone
(TQ)
on
autophagy
via
mitochondrial
function
after
modulation
Wnt/β-catenin
signaling
pathway.
Human
colorectal
adenocarcinoma
HT-29
cells
were
treated
TQ
(60
µM)
and
15
µM
Wnt3a
inhibitor
(LGK974)
for
48
h.
The
survival
rate
was
evaluated
using
an
MTT
assay.
expression
Wnt-related
factors
(c-Myc,
Axin),
angiogenesis
(VE-Cadherin),
mitophagy-related
(PINK1,
OPTN)
assessed
real-time
PCR
Protein
levels
(Beclin-1,
LC3,
P62)
western
blotting.
Using
flow
cytometry
analysis,
intracellular
accumulation
Rhodamine
123
evaluated.
migration
properties
analyzed
a
scratch
wound
healing
Data
indicated
that
can
reduce
viability
compared
to
control
(
p
<
0.05).
VE-Cadherin
inhibited
while
PINK1
induced
Both
LGK974
TQ-treated
exhibited
activation
flux
(Beclin-1↑,
LC3II/I↑,
p62↓)
group
increase
123,
indicating
inhibition
efflux
mechanisms
cells.
Along
these
changes,
also
reduced
potential
phytocompound
alter
dynamic
growth
human
autophagy,
mechanisms.
