Development of endosome-related gene signature for the prediction of prognosis and therapeutic response in breast cancer
Medicine,
Год журнала:
2025,
Номер
104(2), С. e41230 - e41230
Опубликована: Янв. 10, 2025
Endosomes
play
a
pivotal
role
in
cellular
biology,
orchestrating
processes
such
as
endocytosis,
molecular
trafficking,
signal
transduction,
and
recycling
of
materials.
This
study
aims
to
construct
an
endosome-related
gene
(ERG)-derived
risk
signature
for
breast
cancer
prognosis.
Transcriptomic
clinical
data
were
retrieved
from
The
Cancer
Genome
Atlas
the
University
California
Santa
Cruz
databases
build
validate
model.
A
Lasso
Cox
regression
model
was
employed
construction.
immune
landscape
assessed
using
CIBERSORT
ESTIMATE
algorithms,
while
drug
sensitivity
evaluated
via
pRRophetic
algorithm.
Gene
set
enrichment
analysis
variation
applied
evaluate
expression
patterns.
nomogram
constructed
validated
predicting
outcomes.
ERGs
cells
tissues
further
validated.
Sixty-one
associated
with
prognosis
identified,
23
selected
constructing
signature.
stratified
patients
into
high-
low-risk
groups,
where
group
exhibited
significantly
better
Notably,
younger
tended
have
lower
scores
compared
older
ones.
enhanced
majority
drugs
tested,
accompanied
by
increased
infiltration
T
M1
macrophages.
Additionally,
cell
cycle
pathways
suppressed
group,
whereas
antigen
binding
functions
activated.
Ultimately,
score
age
identified
independent
prognostic
factors
cancer,
these
incorporated
that
demonstrated
excellent
performance
assessment.
Finally,
external
cohort
dysregulation
score-associated
tissues.
successfully
established
ERG-derived
nomogram,
elucidating
their
potential
value
prediction
evaluation
therapeutic
response.
Язык: Английский
Genes Associated with the Immune System Affected by Ionizing Radiation and Estrogen in an Experimental Breast Cancer Model
Biology,
Год журнала:
2024,
Номер
13(12), С. 1078 - 1078
Опубликована: Дек. 20, 2024
Breast
cancer
is
a
global
health
issue
that,
when
in
the
metastasis
stage,
characterized
by
lack
of
estrogen
receptor-α,
progesterone
receptor,
and
human
epidermal
growth
receptor
expressions.
The
present
study
analyzed
differential
gene
expression
related
to
immune
system
affected
ionizing
radiation
cell
lines
derived
from
an
experimental
breast
model
that
was
previously
developed;
where
immortalized
epithelial
line
MCF-10F,
triple-negative
line,
exposed
low
doses
high
linear
energy
transfer
α
particle
(150
keV/μm),
it
subsequently
grew
presence
or
absence
17β-estradiol.
Results
indicated
interferon-related
developmental
regulator
1
estrogen-treated
line;
this
interferon,
as
well
Interferon-Induced
Transmembrane
protein
2,
TNF
alpha-induced
Protein
6
levels
were
higher
than
control
Alpha3
line.
Furthermore,
1,
6,
Nuclear
Factor
Interleukin
3-regulated,
Interferon-Gamma
Receptor
showed
Alpha5
Interferon
Regulatory
Tumor2
Additionally,
further
strengthen
these
data,
publicly
available
datasets
analyzed.
This
analysis
conducted
assess
correlation
between
alpha
genes
mentioned
above
patients,
tumor
normal
tissues,
infiltration
level,
ER
status,
survival
outcome
adjusted
clinical
stage
factor.
It
can
be
concluded
interferon
family
Tumor
Necrosis
factors
potential
therapeutic
targets
for
cancer,
since
they
are
active
before
formation
defense
body
under
effects.
Язык: Английский
Affinity of PET-MRI Tracers for Hypoxic Cells in Breast Cancer: A Systematic Review
Cells,
Год журнала:
2024,
Номер
13(12), С. 1048 - 1048
Опубликована: Июнь 17, 2024
Tumour
hypoxia
is
a
known
microenvironmental
culprit
for
treatment
resistance,
tumour
recurrence
and
promotion
of
metastatic
spread.
Despite
the
long-known
existence
this
factor
within
milieu,
still
one
greatest
challenges
in
cancer
management.
The
transition
from
invasive
less
reliable
detection
methods
to
more
accurate
non-invasive
ways
identify
quantify
was
long
process
that
eventually
led
promising
results
showed
by
functional
imaging
techniques.
Hybrid
imaging,
such
as
PET-CT,
has
great
advantage
combining
structural
or
anatomical
image
(offered
CT)
with
metabolic
PET).
However,
context
hypoxia,
it
only
PET
taken
after
appropriate
radiotracer
administration
would
supply
hypoxia-specific
information.
To
overcome
limitation,
development
latest
hybrid
systems,
PET-MRI,
enables
synergistic
approach
towards
both
having
potential
provide
information
on
microenvironment.
This
study
designed
systematic
review
literature
newest
developments
PET-MRI
hypoxic
cells
breast
cancer.
analysis
includes
affinity
various
tracers
patient
group
well
correlations
between
PET-specific
MRI-specific
parameters,
offer
broader
view
widespread
clinical
implementation
technique.
Язык: Английский
Morphological assessment of angiogenesis factor expression in tumor and microenvironment of breast fibroadenoma and ductal carcinoma: An observational cohort study
Kuban Scientific Medical Bulletin,
Год журнала:
2024,
Номер
31(5), С. 26 - 40
Опубликована: Окт. 25, 2024
Background
.
Angiogenesis
plays
a
crucial
role
in
the
progression
of
breast
cancer.
Identifying
and
investigating
key
components
this
process,
focused
on
phenotype
as
well
microenvironment
tumor,
is
considered
highly
relevant
for
understanding
tumor
biology.
Studies
into
expression
angiogenesis-related
factors
by
means
immunohistochemical
methods
appear
valuable
both
assessing
conventional
chemotherapy
options
identifying
new
targets
targeted
therapy
Objectives
To
investigate
angiogenesis
ductal
carcinoma
vascular
endothelial
growth
factor,
angiopoietin-2,
hypoxia-inducible
factor
alpha
context
various
therapeutic
strategies.
Methods
An
observational
cohort
study
was
conducted
using
biopsy
samples
from
female
patients
with
confirmed
diagnoses
“fibroadenoma”
“ductal
breast,”
residents
Republic
Crimea,
who
applied
to
oncological
hospitals
Simferopol
January
2021
2023.
Examination
involved
histological
sections
tissue
68
verified
carcinoma”
(the
mean
age
65
±
5).
The
following
cohorts
were
formed
study:
control
group,
consisting
fibroadenoma
(
n
=
20);
two
subgroups
48),
including
Group
I
—
had
not
received
23),
II
breast,
underwent
surgery
one
or
more
courses
25).
examining
obtained
paraffin
blocks,
markers
via
immunohistochemistry
primary
antibodies
against
angiopoietin
2,
alpha.
Statistical
analysis
carried
out
Statistica
10.0
(StatSoft,
USA).
Differences
significant
at
error
probability
p
≤
0.05.
value
<
0.05
deemed
statistically
all
types
analysis.
Results
differed
significantly
between
groups
when
compared
group.
having
cytoplasmic
localization
detected
group
benign
processes,
whereas
nuclear
noted
groups.
Significant
differences
have
been
established
among
breast:
II,
which
chemotherapy,
notably
higher
stroma
tumor-free
areas.
greater
demarcation
zone
than
that
observed
surgically
treated
women
0.033;
0.034,
0.001,
respectively).
In
epithelium
carcinoma,
expressed
intensively
did
receive
other
0.001).
Conversely,
stroma,
exhibited
levels
those
no
treatment;
areas
due
cell
involvement
0.004)
conditionally
healthy
regions
represented
patients,
studied
pronounced
breast.
Conclusion
data
indicate
activation
processes
after
evidenced
increased
angiopoietin.
This
result
associated
high
prevalence
resistant
forms
II.
signaling
pathways
its
provides
insights
patterns
occurrence
strategies
overcome
resistance
Язык: Английский
DNA Damage and Inflammatory Response of p53 Null H358 Non-Small Cell Lung Cancer Cells to X-Ray Exposure Under Chronic Hypoxia
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12590 - 12590
Опубликована: Ноя. 23, 2024
Hypoxia-induced
radioresistance
limits
therapeutic
success
in
cancer.
In
addition,
p53
mutations
are
widespread
tumors
including
non-small
cell
lung
carcinomas
(NSCLCs),
and
they
might
modify
the
radiation
response
of
hypoxic
tumor
cells.
We
therefore
analyzed
DNA
damage
inflammatory
chronically
(1%
O
Язык: Английский
PRKD3 promotes proliferation of liver cancer cells: a downstream proteomics profiling study
Ye Tian,
Bei Xie,
Shuaiyang Wang
и другие.
American Journal of Translational Research,
Год журнала:
2024,
Номер
16(11), С. 6384 - 6398
Опубликована: Янв. 1, 2024
Protein
kinase
D
3
(PRKD3),
a
serine/threonine
protein
kinase,
functions
as
crucial
regulator
across
numerous
cancer
types.
However,
its
regulatory
function
and
mechanism
in
hepatocellular
carcinoma
(HCC)
proliferation
remain
unclear.
In
vitro
experiments
proteomics
analysis
offer
new
insights
into
the
regulation
of
PRKD3
HCC.
A
knockdown
cell
line
was
constructed
to
assess
effects
on
HCC
cells
using
counting
kit-8
(CCK-8)
assay,
5-Ethynyl-2'-deoxyuridine
(EdU)
clonogenic
flow
cytometry.
Proteomic
changes
liver
before
after
were
analyzed
4D-lablefree
technology.
Analysis
The
Cancer
Genome
Atlas
(TCGA)
dataset
revealed
abnormal
expression
HCC,
associated
with
poorer
prognosis
specific
pathological
Results
from
CCK-8
assay
showed
marked
reduction
Huh7
(P
<
0.01),
number
clonal
colonies
being
5.26
times
higher
than
that
cells,
EdU
positivity
rate
decreased
54.77%
37.97%.
Flow
cytometry
results
indicated
knockout
induced
cycle
arrest
at
G2/M
phase.
330
proteins
had
altered
expression,
amino
acid
transport,
stress
response,
apoptosis.
Cyclin-dependent
4
(CDK4),
plasminogen
activator
inhibitor
1
(SERPINE1),
sequestosome
(SQSTM1),
ras-related
Rab-8A
(RAB8A),
nuclear
receptor-binding
factor
2
(NRBF2)
emerged
key
nodes
interaction
network.
This
study
elucidates
inhibitory
effect
unveils
proteomic
features
regulation.
CDK4,
SERPINE1,
SQSTM1,
RAB8A,
NRBF2
may
serve
PRKD3's
pathways.
Язык: Английский