Yangke powder alleviates OVA-induced allergic asthma by inhibiting the PI3K/AKT/NF-κB signaling pathway DOI Creative Commons
Xueyan Li, Lu Ding, Zirui Li

и другие.

Chinese Medicine, Год журнала: 2025, Номер 20(1)

Опубликована: Май 26, 2025

Abstract Background Asthma is a chronic inflammatory airway disease that remains inadequately controlled by existing conventional treatments. A traditional Chinese medicine (TCM) formula of Yangke powder (yǎng ké sǎn-YKS) has demonstrated potential in alleviating asthma symptoms and reducing its acute exacerbation. Despite clinical evidence supporting benefit, there still insufficient understanding the active compounds YKS their underlying mechanisms, which limits broader application. Objective This study aims to identify key ingredients explore particularly through PI3K/AKT/NF-κB pathways, provide scientific basis for application treatment. Methods We employed UPLC-Q-Exactive Orbitrap-MS analyze constituents, identified ingredients, explored treatment mechanisms bioinformatics, network pharmacology, Mendelian randomization, molecular docking. The model was evaluated using ovalbumin (OVA) pulmonary function tests, while pathological examination conducted hematoxylin eosin (HE), periodic acid-Schiff (PAS), Masson trichrome stains. Concentrations IgE, IL-4, IL-5 were measured ELISA, protein mRNA expressions confirmed via qPCR, immunohistochemistry, Western blot analysis. Results total 174 UPLC-MS, with 49 detected bloodstream, indicating role as ingredients. Bioinformatics analysis revealed 353 asthma-related targets 972 YKS. Key such AKT1, TNF, IL1B validated Our studies indicated modulates primarily PI3K/Akt NF-κB improving resistance, inflammation, mucus production, remodeling, decreasing Th2 cytokines IgE levels. Conclusion investigation identifies Kaempferol, Norephedrine, Cynaroside, Genistein, Rutin critical YKS, impacting biomarkers IL1B. These substances effectively modulate pathway, enhancing management allergic asthma.

Язык: Английский

Yangke powder alleviates OVA-induced allergic asthma by inhibiting the PI3K/AKT/NF-κB signaling pathway DOI Creative Commons
Xueyan Li, Lu Ding, Zirui Li

и другие.

Chinese Medicine, Год журнала: 2025, Номер 20(1)

Опубликована: Май 26, 2025

Abstract Background Asthma is a chronic inflammatory airway disease that remains inadequately controlled by existing conventional treatments. A traditional Chinese medicine (TCM) formula of Yangke powder (yǎng ké sǎn-YKS) has demonstrated potential in alleviating asthma symptoms and reducing its acute exacerbation. Despite clinical evidence supporting benefit, there still insufficient understanding the active compounds YKS their underlying mechanisms, which limits broader application. Objective This study aims to identify key ingredients explore particularly through PI3K/AKT/NF-κB pathways, provide scientific basis for application treatment. Methods We employed UPLC-Q-Exactive Orbitrap-MS analyze constituents, identified ingredients, explored treatment mechanisms bioinformatics, network pharmacology, Mendelian randomization, molecular docking. The model was evaluated using ovalbumin (OVA) pulmonary function tests, while pathological examination conducted hematoxylin eosin (HE), periodic acid-Schiff (PAS), Masson trichrome stains. Concentrations IgE, IL-4, IL-5 were measured ELISA, protein mRNA expressions confirmed via qPCR, immunohistochemistry, Western blot analysis. Results total 174 UPLC-MS, with 49 detected bloodstream, indicating role as ingredients. Bioinformatics analysis revealed 353 asthma-related targets 972 YKS. Key such AKT1, TNF, IL1B validated Our studies indicated modulates primarily PI3K/Akt NF-κB improving resistance, inflammation, mucus production, remodeling, decreasing Th2 cytokines IgE levels. Conclusion investigation identifies Kaempferol, Norephedrine, Cynaroside, Genistein, Rutin critical YKS, impacting biomarkers IL1B. These substances effectively modulate pathway, enhancing management allergic asthma.

Язык: Английский

Процитировано

0