Vibrational Spectroscopy,
Год журнала:
2024,
Номер
132, С. 103671 - 103671
Опубликована: Март 12, 2024
Vibrational
spectroscopy,
largely
based
on
infrared
absorption
and
Raman
scattering
techniques,
is
much
vaunted
as
a
label
free
approach,
delivering
high
content,
holistic
characterisation
of
sample,
with
demonstrable
applications
in
broad
range
fields,
from
process
analytical
technologies
preclinical
drug
screening,
to
disease
diagnostics,
therapeutics,
prognostics
personalised
medicine.
However,
the
analysis
such
complex
systems,
trend
has
emerged
which
spectral
reduced
identification
individual
peaks,
reference
tables
assignments
derived
literature,
are
then
interpreted
biomarkers.
More
sophisticated
attempts
unmix
spectrum
mixture
into
constituent
components,
used
characterise
biochemistry
sample
changes
it,
terms
its
components.
Data
mining
spectra,
particular
change
due
kinetic
processes,
remains
challenge,
it
proposed
that
rate
temporal
evolution
combination
can
be
itself
by
guide
analysis.
Ultimately,
argued
true
potential
spectroscopy
best
harnessed
truly
"spectralomic"
signature
an
"event",
intercalation
DNA
nucleus
cell,
or
key
stage
cellular
pathway
oxidative
stress,
presented.
It
envisioned
that,
future,
Spectralomics
will
fully
integrated
similar
omics
approaches,
potentially
ultimately
through
deep
learning
algorithms,
underpinned
systems
biology
models,
provide
living
human
cell
atlas,
describing
function
dysfunction
organism
at
level,
basis
for
improved
healthcare.
Advanced Drug Delivery Reviews,
Год журнала:
2024,
Номер
207, С. 115195 - 115195
Опубликована: Фев. 5, 2024
Enhanced
targeting
approaches
will
support
the
treatment
of
diseases
associated
with
dysfunctional
mitochondria,
which
play
critical
roles
in
energy
generation
and
cell
survival.
Obstacles
to
mitochondria-specific
include
presence
distinct
biological
barriers
need
pass
through
(or
avoid)
various
internalization
mechanisms.
A
range
studies
have
reported
design
mitochondrially-targeted
nanomedicines
that
navigate
complex
routes
required
influence
mitochondrial
function;
nonetheless,
a
significant
journey
lies
ahead
before
become
suitable
for
clinical
use.
Moving
swiftly
forward
require
safety
studies,
vivo
assays
confirming
effectiveness,
methodologies
validate
mitochondria-targeted
nanomedicines'
subcellular
location/activity.
From
nanomedicine
standpoint,
we
describe
involved
(from
administration
arrival
within
mitochondria),
features
influencing
rational
design,
techniques
used
identify/validate
successful
targeting.
Overall,
rationally-designed
mitochondria-targeted-based
hold
great
promise
precise
therapeutic
delivery.
Metabolites,
Год журнала:
2024,
Номер
14(1), С. 54 - 54
Опубликована: Янв. 14, 2024
Blood
metabolomics
profiling
using
mass
spectrometry
has
emerged
as
a
powerful
approach
for
investigating
non-cancer
diseases
and
understanding
their
underlying
metabolic
alterations.
Blood,
readily
accessible
physiological
fluid,
contains
diverse
repertoire
of
metabolites
derived
from
various
systems.
Mass
offers
universal
precise
analytical
platform
the
comprehensive
analysis
blood
metabolites,
encompassing
proteins,
lipids,
peptides,
glycans,
immunoglobulins.
In
this
review,
we
present
an
overview
research
landscape
in
spectrometry-based
profiling.
While
field
is
primarily
focused
on
cancer,
review
specifically
highlights
studies
related
to
diseases,
aiming
bring
attention
valuable
that
often
remains
overshadowed.
Employing
natural
language
processing
methods,
processed
507
articles
provide
insights
into
application
metabolomic
specific
The
encompasses
wide
range
with
emphasis
cardiovascular
disease,
reproductive
diabetes,
inflammation,
immunodeficiency
states.
By
analyzing
samples,
researchers
gain
perturbations
associated
these
potentially
leading
identification
novel
biomarkers
development
personalized
therapeutic
approaches.
Furthermore,
approaches
utilized
research,
including
GC-MS,
LC-MS,
others
discussing
advantages
limitations.
To
enhance
scope,
propose
recent
supporting
applicability
GC×GC-MS
metabolomics-based
studies.
This
addition
will
contribute
more
exhaustive
available
techniques.
Integration
clinical
practice
holds
promise
improving
disease
diagnosis,
treatment
monitoring,
patient
outcomes.
unraveling
complex
alterations
healthcare
professionals
can
pave
way
precision
medicine
interventions.
Continuous
advancements
technology
data
methods
further
potential
facilitating
its
translation
laboratory
routine
application.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(4), С. 1783 - 1783
Опубликована: Фев. 19, 2025
This
study
examines
the
effects
of
metformin
on
brain
functions
focusing
variability
results
reported
in
literature.
While
some
studies
suggest
that
may
have
neuroprotective
diabetic
patients,
others
report
an
insignificant
impact
cognitive
function,
or
even
a
negative
effect.
We
propose
this
inconsistency
be
due
to
intrinsic
cellular-level
among
individuals,
which
we
term
"biovariance".
Biovariance
persists
demographically
homogeneous
samples
complex
and
stochastic
biological
processes.
Additionally,
metabolic
actions
metformin,
including
its
influence
neuroenergetics
neuronal
survival,
produce
different
depending
individual
characteristics.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Июль 14, 2023
Abstract
Mammalian
retinal
metabolism
favors
aerobic
glycolysis.
However,
the
role
of
glycolytic
in
morphogenesis
remains
unknown.
We
report
that
glycolysis
is
necessary
for
early
stages
development.
Taking
advantage
an
unbiased
approach
combines
use
eye
organoids
and
single-cell
RNA
sequencing,
we
identify
specific
glucose
transporters
genes
progenitors.
Next,
determine
optic
vesicle
territory
mouse
embryos
displays
elevated
levels
activity.
At
functional
level,
show
removal
Glucose
transporter
1
Lactate
dehydrogenase
A
gene
activity
from
developing
progenitors
arrests
morphogenesis.
Surprisingly,
uncover
lactate-mediated
upregulation
key
eye-field
transcription
factors
controlled
by
epigenetic
modification
histone
H3
acetylation
through
deacetylase
Our
results
unexpected
bioenergetic
independent
lactate
as
a
signaling
molecule
mammalian
Annual Review of Nutrition,
Год журнала:
2023,
Номер
43(1), С. 153 - 177
Опубликована: Авг. 21, 2023
Gluconeogenesis
is
a
critical
biosynthetic
process
that
helps
maintain
whole-body
glucose
homeostasis
and
becomes
altered
in
certain
medical
diseases.
We
review
gluconeogenic
flux
various
diseases,
including
common
metabolic
disorders,
hormonal
imbalances,
specific
inborn
genetic
errors,
cancer.
discuss
how
the
activity
contributes
to
disease
pathogenesis
using
data
from
experiments
isotopic
tracer
spectroscopy
methodologies.
These
vitro,
animal,
human
studies
provide
insights
into
changes
circulating
levels
of
available
gluconeogenesis
substrates
efficiency
converting
those
by
organs.
highlight
ongoing
knowledge
gaps,
emerging
research
areas,
suggest
future
investigations.
A
better
understanding
may
ultimately
identify
novel
targeted
treatment
strategies
for
such
Critical Reviews in Microbiology,
Год журнала:
2024,
Номер
unknown, С. 1 - 40
Опубликована: Янв. 25, 2024
Microbial
communities
thrive
through
interactions
and
communication,
which
are
challenging
to
study
as
most
microorganisms
not
cultivable.
To
address
this
challenge,
researchers
focus
on
the
extracellular
space
where
communication
events
occur.
Exometabolomics
interactome
analysis
provide
insights
into
molecules
involved
in
dynamics
of
their
interactions.
Advances
sequencing
technologies
computational
methods
enable
reconstruction
taxonomic
functional
profiles
microbial
using
high-throughput
multi-omics
data.
Network-based
approaches,
including
community
flux
balance
analysis,
aim
model
molecular
within
between
communities.
Despite
these
advances,
challenges
remain
computer-assisted
biosynthetic
capacities
elucidation,
requiring
continued
innovation
collaboration
among
diverse
scientists.
This
review
provides
current
state
future
directions
elucidation
studying
Analytical Science Advances,
Год журнала:
2024,
Номер
5(5-6)
Опубликована: Июнь 1, 2024
Abstract
The
field
of
metabolomics
has
gained
tremendous
interest
in
recent
years.
Whether
the
goal
is
to
discover
biomarkers
related
certain
pathologies
or
better
understand
impact
a
drug
contaminant,
numerous
studies
have
demonstrated
how
crucial
it
variations
metabolism.
Detailed
knowledge
metabolic
variabilities
can
lead
more
effective
treatments,
as
well
faster
less
invasive
diagnostics.
Exploratory
approaches
are
often
employed
metabolomics,
using
relative
quantitation
look
at
perturbations
between
groups
samples.
Most
been
based
on
metabolite
profiling
quantitation,
with
very
few
an
approach
for
absolute
quantitation.
Using
accurate
facilitates
comparison
different
studies,
enabling
longitudinal
studies.
In
this
review,
we
discuss
most
widely
used
techniques
quantitative
mass
spectrometry
(MS).
Various
aspects
will
be
addressed,
such
use
external
and/or
internal
standards,
derivatization
techniques,
vivo
isotopic
labelling,
MS
imaging.
principles,
associated
limitations
and
challenges,
described
each
approach.
