Heliyon, Год журнала: 2024, Номер 10(20), С. e39584 - e39584
Опубликована: Окт. 1, 2024
Язык: Английский
ACS Applied Bio Materials, Год журнала: 2023, Номер 7(1), С. 44 - 58
Опубликована: Дек. 18, 2023
Epithelial-mesenchymal transition (EMT) is a fundamental process driving cancer metastasis, transforming non-motile cells into motile population that migrates to distant organs and forms secondary tumors. In recent years, research has revealed strong connection between exosomes the EMT. Exosomes, subpopulation of extracellular vesicles, facilitate cellular communication dynamically regulate various aspects including immune cell suppression, matrix remodeling, metastasis initiation, EMT organ-specific metastasis. Tumor-derived (TEXs) their molecular cargo, comprising proteins, lipids, nucleic acids, carbohydrates, are essential components promote in cancer. TEXs miRNAs play crucial role reprogramming tumor microenvironment, while TEX surface integrins contribute Exosome-based offers deeper understanding about an effective theranostic platform development. Additionally, therapeutic sources paving way for innovative treatment this Review, we spotlight impact, aiming inspire researchers worldwide explore fascinating field more ways.
Язык: Английский
Процитировано
21
Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11
Опубликована: Авг. 31, 2023
Intrauterine adhesion (IUA), also referred to as Asherman Syndrome (AS), results from uterine trauma in both pregnant and nonpregnant women. The IUA damages the endometrial bottom layer, causing partial or complete occlusion of cavity. This leads irregular menstruation, infertility, repeated abortions. Transcervical electroreception (TCRA) is frequently used treat IUA, which greatly lowers prevalence adhesions increases pregnancy rates. Although surgery aims disentangle adhesive tissue, it can exacerbate development when degree severer. Therefore, critical develop innovative therapeutic approaches for prevention IUA. Endometrial fibrosis essence studies have found that use different types mesenchymal stem cells (MSCs) reduce risk increase possibility pregnancy. Recent research has suggested exosomes derived MSCs overcome limitations MSCs, such immunogenicity tumorigenicity risks, thereby providing new directions treatment. Moreover, hydrogel drug delivery system significantly ameliorate recurrence rate intrauterine patients, its potential mechanism treatment been studied. It shown combination two more schemes broader application prospects; therefore, this article reviews pathophysiology current strategies, focusing on combined with hydrogels certain challenges treating they still provide promising field.
Язык: Английский
Процитировано
20
Cells, Год журнала: 2023, Номер 12(2), С. 311 - 311
Опубликована: Янв. 13, 2023
Ferroptosis is a type of iron-dependent cell death caused by ferrous iron overload, reactive oxygen species generation through the Fenton reaction, and lipid peroxidation, leading to antioxidative system dysfunction and, ultimately, membrane damage. The functional role ferroptosis in human physiology pathology considered cause or consequence diseases. Circulating exosomes mediate intercellular communication organ crosstalk. They not only transport proteins nucleic acids derived from parental cells but also serve as vehicles for targeted delivery exogenous cargo. Exosomes regulate delivering biological material recipient cell, affecting ferroptosis-related proteins, transporting ferritin-bound out cell. This review discusses pathogenesis mediated endogenous therapeutic potential In addition, this explores exosome-mediated diseases with an emphasis on strategies engineering therapy.
Язык: Английский
Процитировано
18
Frontiers of Medicine, Год журнала: 2024, Номер 18(2), С. 258 - 284
Опубликована: Янв. 13, 2024
Язык: Английский
Процитировано
6
ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 10(4), С. 2235 - 2250
Опубликована: Март 6, 2024
The use of exosomes to relieve skin injuries has received considerable attention. PluronicF-127 hydrogel (PF-127 hydrogel) is a novel biomaterial that can be used carry biomolecules. This study sought investigate the impact originating from human mesenchymal stem cells (MSCs) developed adipose tissue (hADSC-Exos) combined with PF-127 on repair and explore underlying mechanism using in vitro vivo experiments. miR-148a-3p most expressed microRNA (miRNA) hADSC-Exos. We found had better efficacy than alone; moreover, knockdown lowered its efficacy. In vitro, we observed significant increase tumor-like ability HUVECs after exosome treatment, which was attenuated knockdown. Furthermore, effects hADSC-Exos were achieved through prevention PTEN triggering phosphatidylinositol 3-kinase (PI3K)/Akt signaling. conclusion, our results demonstrated promote angiogenesis wound healing by delivering have effect when hydrogel, may an alternative strategy healing.
Язык: Английский
Процитировано
6
Cancers, Год журнала: 2023, Номер 15(7), С. 2156 - 2156
Опубликована: Апрель 5, 2023
Exosomes are nanosized vesicles that produced in normal and cancer cells, promoting intracellular communication. In head neck (HNC), exosomes involved many undesirable events of development progression, including angiogenesis, tumor microenvironment (TME) remodeling, invasion, epithelial-to-mesenchymal transition (EMT), metastasis, extracellular matrix (ECM) degradation, drug resistance. altering the signaling pathways recipient cells by cargoes they carry. Proteins, lipids, nucleic acids such as DNA fragments RNAs (i.e., mRNAs, miRNAs, long non-coding RNAs) carried to promote cell EMT is a critical cellular process which epithelial forced become mesenchymal actions SNAIL/SLUG, TWIST, ZEB family transcription factors facilitate metastasis. this review, we focused on exosome biogenesis, their cargoes, involvement induction metastasis during HNC. Insights into isolation characterization, well key role ECM remodeling also presented critically discussed. More importantly, article addresses HNC resistance induced drug-sensitive cells. addition, have great potential be used diagnostic therapeutic tools. A better understanding composition, functions will aid developing novel strategies treat HNC, overcome therapy resistance, avoid significant cause death.
Язык: Английский
Процитировано
13
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 9871 - 9887
Опубликована: Сен. 1, 2024
Pathological scarring results from aberrant cutaneous wound healing due to the overactivation of biological behaviors human skin fibroblasts, characterized by local inordinate inflammation, excessive extracellular matrix and collagen deposition. Yet, its underlying pathogenesis opinions vary, which could be caused increased mechanical tension, enhanced continuous gene mutation, as well cellular metabolic disorder, etc. Metabolic reprogramming is process pattern cells undergoes a systematic adjustment transformation adapt changes external environment meet needs their growth differentiation. Therefore, abnormality in within wounds scars attaches great importance scar formation. Mesenchymal stem cells-derived exosomes (MSC-Exo) are vesicles that play an important role tissue repair, cancer treatment immune regulation. However, there not work detail relevant studies. Herein, we gave comprehensive summary existing research on three main metabolisms, including glycometabolism, lipid metabolism amino acid metabolism, MSC-Exo regulating formation for further reference.
Язык: Английский
Процитировано
5
Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)
Опубликована: Дек. 3, 2024
Minor salivary gland mesenchymal stem cells (MSGMSCs) can be easily extracted and have a broad range of sources. Applying exosomes to wounds is highly promising method for promoting wound healing. Exosomes derived from different cell types been proven enhance healing, with adipose-derived (ADSC)-derived being the most extensively researched. Considering that MSGMSCs advantages such as easier extraction compared ADSCs, should also very type in exosome therapy. However, whether MSGMSC-derived (MSGMSC-exos) promote healing how they compare ADSC-derived (ADSC-exos) process remain unclear. The effects MSGMSC-exos ADSC-exos on angiogenesis were investigated vitro using CCK-8, scratch assays, tube formation assays. Subsequently, promotion by was evaluated vivo full-thickness defect model mice. Immunohistochemistry used verify collagen deposition, angiogenesis, proliferation wound. Immunofluorescence staining performed investigate role modulating inflammatory response Furthermore, proteomic sequencing conducted functional similarities differences between proteomes ADSC-exos, key protein contents verified ELISA. exhibited similar migration, proliferation, human umbilical vein endothelial (HUVECs) vitro, comparable dose-dependent effect. In experiments confirmed healing-promoting functions ADSC-exos. promoted neovascularization maturation blood vessels at level Despite showing less deposition than stronger anti-scar anti-inflammatory effects. Proteomic analysis revealed proteins both relatively conserved, ITGB1 identified critical angiogenesis. Further differential specifically enriched reflected their source tissue. Our study confirms exhibit angiogenesis-promoting involved are conserved. Moreover, show This suggests applications treatment.
Язык: Английский
Процитировано
3
Regenerative Engineering and Translational Medicine, Год журнала: 2025, Номер unknown
Опубликована: Фев. 3, 2025
Язык: Английский
Процитировано
0
Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)
Опубликована: Фев. 11, 2025
The self-renewal ability and multipotency of stem cells give them great potential for use in wound healing. Stem cell-derived exosomes, owing to their close biological resemblance parent cells, offer a more efficient, safer, economical approach facilitating cellular communication interactions within different environments. This makes particularly valuable the treatment both acute chronic wounds, such as lacerations, burns, diabetic ulcers. Long non-coding RNAs (lncRNAs) enclosed one leading actors these extracellular microvesicles, through interaction with miRNAs regulation various signaling pathways involved inflammation, angiogenesis, cell proliferation, migration, could heal wounds. Exosome-derived lncRNAs from facilitate matrix remodeling between macrophages fibroblasts. Moreover, alongside regulating expression inflammatory cytokines, controlling reactive oxygen species levels, enhancing autophagic activity, they also modulate immune responses support Regulating genes related by increasing blood supply accelerating delivery essential substances environment, is another effect exosomal derived These can enhance skin healing homeostasis, proliferation differentiation wound-healing process, fibroblast viability migration injury site. Ultimately, exosome-derived novel insights into molecular mechanisms underlying improved They pave way therapeutic strategies, fostering further research better future. Meanwhile, exosomes mesenchymal due exceptional regenerative properties, well have emerged innovative tools review article aims narrate roles focus on cells.
Язык: Английский
Процитировано
0