High-throughput Bronchus-on-a-Chip system for modeling the human bronchus

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 1, 2024

Airway inflammation, a protective response in the human body, can disrupt normal organ function when chronic, as seen chronic obstructive pulmonary disease (COPD) and asthma. Chronic bronchitis induces goblet cell hyperplasia metaplasia, obstructing airflow. Traditional animal testing is often replaced by vitro three-dimensional cultures of epithelial cells to assess responses. However, these are cultured horizontally, differing from tubular structure airway failing accurately reproduce stenosis. To address this, we developed Bronchus-on-a-Chip (BoC) system. The BoC uses novel microfluidic design standard laboratory plate, embedding 62 chips one plate. Human bronchial were against collagen extracellular matrix for up 35 days. Characterization included barrier integrity assays, microscopy, histological examination. Cells successfully structure, with apical side air-lifted. Epithelial differentiated into basal, ciliated, secretory cells, mimicking epithelium. Upon exposure inducers system showed mucus hyperproduction, replicating This enhances providing more human-relevant high-throughput method.

Язык: Английский

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Abstracts from The International Society for Aerosols in Medicine

Journal of Aerosol Medicine and Pulmonary Drug Delivery, Год журнала: 2023, Номер 36(6), С. A - 39

Опубликована: Окт. 31, 2023

Язык: Английский

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Effect of enclosing terpenoids-rich Boswellia Carterri ethyl acetate extract in binary cyclodextrin based oligomer nano-complex for improving its activity via counteracting ICAM-1, Ilβ4 and LTB4 pathways in respiratory distressed rats

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Янв. 8, 2024

Boswellia carterii (BC) resins plants have a long historical background as treatment for inflammation, indicated by information originating from multiple countries. Twenty-seven diterpenoids been identified in ethylacetate and total methanol BC, comprising seventeen boscartins of the cembrane-type ten boscartols prenylaromadendrane-type diterpenoids. Moreover, twenty-one known triterpenoids also found, encompassing nine tirucallane-type, six ursane-type, four oleanane-type, two lupane-type. The hold significant position pharmaceutical chemistry related industries due to their captivating biological characteristics promising pharmacological potentials. Extraction creation assessment nano sponges loaded with either B. Carterii plant extract or DEX, are subjects our current investigation. With use ultrasound-assisted synthesis, were produced. entrapment efficiency (EE%) medications Nano was examined using spectrophotometry. characterized number methods. Within sponges, EE% medicines varied between 98.52± 0.07 99.64± 1.40%. sponges' particle sizes 105.9±15.9 166.8±26.3 nm. Drugs released Korsmeyer-Peppas concept. In respiratory distressed rats, effects BC extract, DEX salt formulations (D1, D5, P1 P1), tested. Treatment significantly reduced ICAM-1, LTB4, ILβ4 levels improved histopathologic profiles, when compared positive control group. Boswellia its sponge formulation showed therapeutic effects. effect may be synergism both formulation. This achieved blocking ICAM-1 LTB4 pathways, therefore counteracting talc powder.

Язык: Английский

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3D bioprinting of the airways and lungs for applications in tissue engineering and in vitro models

Journal of Tissue Engineering, Год журнала: 2024, Номер 15

Опубликована: Янв. 1, 2024

Tissue engineering and in vitro modeling of the airways lungs respiratory system are substantial research clinical importance. In airway lung models aim to improve treatment options for repair advance pathophysiological research. The construction biomimetic native with tissue-specific biological, mechanical, configurable features remains challenging. Bioprinting, an emerging 3D printing technology, is promising development airway, lung, disease models, allowing incorporation cells biologically active molecules into printed constructs a precise reproducible manner recreate airways, architecture, microenvironment. Herein, we present review bioprinting applications tissue modeling. key characteristics interstitium, alveoli described. bioinks recently used summarized. Furthermore, propose bioink categorization based on structural airways. Finally, challenges opportunities biofabrication discussed.

Язык: Английский

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"Lung-on-a-chip" as a tool for studying the pathophysiology of human lungs

Journal of clinical practice, Год журнала: 2024, Номер unknown

Опубликована: Дек. 20, 2024

Lung-on-a-Chip (LoC) is a microfluidic device that simulates the gas-liquid interface of human pulmonary alveoli and intended for pathophysiological, pharmacological molecular biological studies blood-air barrier in vitro. The LoC includes system liquid gas microchannels separated by semipermeable elastic membrane containing polymer base cellular elements alveoli. Depending on type (single-channel, two-channel three-channel), may contain only alveolocytes, or alveolocytes combination with other cells: endothelial cells, fibroblasts, alveolar macrophages, tumor etc. Some models also include hydrogel stroma interstitium. first LoC, which there monolayer cells one side other, was developed 2010 group Ingber et al. at Wyss Institute Harvard University order to reproduce microenvironment biomechanics Modern modifications same differ design system, biomaterial, composition stromal special tasks being solved. In addition barrier, are studying specific pathophysiological processes, screening drugs, modeling particular diseases, such as lung cancer, COPD asthma. this review, we analyzed existing varieties biomaterials used, methods detecting processes devices main areas research using "Lung-on-a-chip" technology.

Язык: Английский

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