Comparative Metabolomic Analysis of Phenolic Compounds in Different Ramulus Mori (Sangzhi) Based on UPLC‐ESI‐MS/MS

eFood, Год журнала: 2025, Номер 6(2)

Опубликована: Март 11, 2025

ABSTRACT In traditional Chinese medicine, Ramulus mori (Sangzhi) is used to lower blood sugar and treat diseases such as diabetes, its therapeutic effects are attributed an abundance of bioactive compounds. This study employed a comparative analysis from three geographical regions within China. The samples included Morus atropurpurea Roxb Guangdong (GDS), australis Poir Jiangsu (JS), nigra Linn, which native Xinjiang (XJYS). discovered significant differences in total polyphenol flavonoid content among various (Sangzhi), with those XJYS showing the highest levels. Utilizing UPLC‐ESI‐MS/MS technology, 128 phenolic metabolites were identified, 17 differential screened. Comparison GDS, JS, exhibited 12 10 different metabolites, respectively, 8 observed between JS XJYS. Furthermore, higher than other two varieties. Additionally, some upregulated have been confirmed possess variety pharmacological health benefits. Kyoto Encyclopedia Genes Genomes linked these biosynthesis, qRT‐PCR corresponding gene expression patterns. These findings provided theoretical basis for use pharmaceutical food industries.

Язык: Английский

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Computational Approaches to Evaluate the Acetylcholinesterase Binding Interaction with Taxifolin for the Management of Alzheimer’s Disease

Molecules, Год журнала: 2024, Номер 29(3), С. 674 - 674

Опубликована: Янв. 31, 2024

Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are enzymes that break down reduce the level of neurotransmitter acetylcholine (ACh). This can cause a variety cognitive neurological problems, including Alzheimer's disease. Taxifolin is natural phytochemical generally found in yew tree bark has significant pharmacological properties, such as being anti-cancer, anti-inflammatory, antioxidant. The binding affinity inhibitory potency taxifolin to these were evaluated through molecular docking dynamics simulations followed by MMPBSA approach, results significant. Taxifolin's for AChE-taxifolin complex was -8.85 kcal/mol, with an inhibition constant 326.70 nM. It observed interact hydrogen bonds. In contrast, BChE-taxifolin energy be -7.42 it significantly nearly equal standard inhibitor donepezil. simulation signified interactions studied enzymes. total free -24.34 while -16.14 kcal/mol. present research suggests strong ability bind inhibit AChE BChE could used manage neuron-associated problems; however, further required explore taxifolin's therapeutic potential using animal models

Язык: Английский

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Biotechnological approaches in the production of plant secondary metabolites for treating human viral diseases: Prospects and challenges

Biocatalysis and Agricultural Biotechnology, Год журнала: 2024, Номер 59, С. 103249 - 103249

Опубликована: Май 22, 2024

Язык: Английский

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Taxifolin regulates SLC31A1-mediated cuproptosis and tumor progression in hepatocellular carcinoma

Human Cell, Год журнала: 2025, Номер 38(2)

Опубликована: Янв. 3, 2025

Язык: Английский

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Parametric optimization, Polyphenol profiling and Bioefficacy of Bacopa monnieri (L.) Pennell phytoextract against multi-drug resistant (MDR) foodborne bacterial pathogens.

Microbial Pathogenesis, Год журнала: 2025, Номер unknown, С. 107320 - 107320

Опубликована: Янв. 1, 2025

Язык: Английский

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Multifunctional CuTax nanozyme-based chitosan edible coatings for fruit preservation

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 143204 - 143204

Опубликована: Апрель 1, 2025

Язык: Английский

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Case Report: Taxifolin for neurosurgery-associated early-onset cerebral amyloid angiopathy

Frontiers in Neurology, Год журнала: 2024, Номер 15

Опубликована: Март 19, 2024

Cases of iatrogenic cerebral amyloid angiopathy (CAA) have been increasingly reported recently, particularly those associated with neurosurgery. Preclinical studies shown taxifolin to be promising for treating CAA. We describe a young 42-year-old man history childhood traumatic brain injury that required craniotomy hematoma evacuation. He later presented recurrent lobar intracerebral hemorrhage (ICH) decades later, which was histologically confirmed Serial 11 C-Pittsburgh compound B positron emission tomography ( C-PiB-PET) imaging showed 24% decrease in global standardized uptake value ratio (SUVR) at 10 months after use. During this period, the patient experienced clinical improvement improved consciousness and reduced ICH frequency, may partly attributable potential amyloid-β (Aβ) clearing effect taxifolin. However, seemed diminished 15 months, CAA should considered patients presenting neurosurgery, serial C-PiB-PET scans warrant further validation as strategy monitoring treatment response candidate Aβ-clearing therapeutic agents such

Язык: Английский

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Analysis of <i>cis</i>-isomer-enriched dihydroquercetin sample by 1D and 2D NMR spectroscopy

Drug development & registration, Год журнала: 2024, Номер unknown

Опубликована: Май 8, 2024

Introduction. The structure of dihydroquercetin (DHQ) is characterized by two chiral centers at positions 2 and 3 the benzopyran cycle, resulting in possible diastereomers: trans - cis -isomers. Therefore, development methods for qualitative quantitative control DHQ diastereomers analyzed samples essential patient safety management. Nuclear magnetic resonance (NMR) spectroscopy one physicochemical that can be used this purpose. Aim. study objective was to accumulate analytical structural characteristics -DHQ NMR spheroidal form flavonoid (DHQs). Materials Methods. 1D 1 H, H-COSY, H-NOESY, 13 C-HSQC spectra were acquired 298 K on an 800 MHz spectrometer equipped with a TXI triple probe. number scans 32. mixing time NOESY experiment 400 ms. H C using CcpNmr software. dihedral angles calculated applying Karplus equation. Results discussion. In -DHQ, chemical shift values H2 H3 are 4.93 ppm 4.52 ppm, respectively, they 5.31 4.20 respectively. spin-spin coupling constants between 12.00 Hz 2.40 Hz, Thus, -isomers 154° 64°, We found DHQs contains 12.5 % -isomer. Conclusion. Our experiments confirmed discriminate based cross-peaks H3. second major finding method considered as more selective analysis than HPLC UV detection without reference. One most important results drug updated information parameters liquid phase.

Язык: Английский

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Optimization of flavanonols heterologous biosynthesis in Streptomyces albidoflavus, and generation of auronols

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Март 28, 2024

Aromadendrin and taxifolin are two flavanonols (derived from the precursor naringenin) displaying diverse beneficial properties for humans. The carbon skeleton of these flavonoids may be transformed by human gastrointestinal microbiota into other compounds, like auronols, which exert different interesting biological activities. While research in has become a certainly extensive field, studies about auronols still scarce. In this work, versions key plant enzyme biosynthesis, flavanone 3-hydroxylase (F3H), been screened selecting best one de novo production compounds bacterial factory Streptomyces albidoflavus UO-FLAV-004-NAR, naringenin overproducer strain. This screening rendered 2.6 μg/L aromadendrin 2.1 mg/L final titers. Finally, expression chalcone isomerase (CHI) gut bacterium Eubacterium ramulus direct conversion (after feeding experiments) 38.1% (+)-aromadendrin maesopsin 74.6% (+)-taxifolin alphitonin. Moreover, heterologous biosynthesis 1.9 alphitonin was accomplished means co-culture strategy producer S. CHI-expressing Escherichia coli, after observation high instability culture medium. study addresses significance time optimization selection appropriate enzymes depending on desired product. To our knowledge, is first that accomplished.

Язык: Английский

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Taxifolin Alleviate Metabolic and Neurochemical Alterations in the Hippocampus and Cortex of Rats with Rotenone-Induced Toxicity: In Vivo and In Silico Insight

Pharmacological Research - Modern Chinese Medicine, Год журнала: 2024, Номер 11, С. 100439 - 100439

Опубликована: Май 8, 2024

: Exposure to rotenone could directly affects the function of mitochondrial which leads neurodegeneration. This study evaluated therapeutic potential taxifolin (Huāqí sōng dài; 花旗松甙) alleviate metabolic and neurochemical alterations in hippocampal cortical region brain rotenone-toxified rats. Male Wistar rats were subcutaneously administered 1.5 mg/kg for 10 days, followed by post-treatment with varying doses (0.25, 0.5 1.0 mg/kg) 3 days through same route. Activities complex I, membrane ion pump lactate dehydrogenase (LDH) hippocampus cortex Markers neurotransmitter metabolism oxidative stress also biochemically estimated molecular interaction between tyrosine hydroxylase, monoamine oxidase, glutamine synthetase Na+/K+ ATPase was determined silico simulation. Taxifolin attenuated dysfunction mitochondrial, ATPase, LDH modulate metabolism. Also, elicited mitigated Furthermore, showed excellent interactions targeted enzymes silico. The highest binding affinity recorded taxifolin-tyrosine hydroxylase complex. Hydrogen bond hydrophobic two key enzymes. alleviates aberrations rotenone-induced neurotoxicity model vivo exhibits promising specific target that are neurotransmission

Язык: Английский

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