Integration of ion-mobility high-resolution liquid chromatography/mass spectrometry-based untargeted metabolomics and desorption electrospray ionization-mass spectrometry imaging to unveil the ginsenosides variation induced by steaming for Panax ginseng, P. quinquefolius and P. notoginseng

Arabian Journal of Chemistry, Год журнала: 2024, Номер 17(6), С. 105781 - 105781

Опубликована: Апрель 7, 2024

The raw and steamed products of ginseng exhibit different efficacy, elucidation the underlying chemical transformation is significant for their rational application quality control. This work was designed to holistically depict variation Panax (PG), P. quinquefolius (PQ), notoginseng (PN) induced by steaming, unveil steaming-associated markers diagnostic differentiating between processed products, following a three-step strategy: 1) systematic ginsenosides characterization hybrid scan (namely HDDIDDA) available on Vion™ ion mobility-quadrupole time-of-flight mass spectrometer coupled with reversed-phase ultra-high performance liquid chromatography; 2) holistic depiction discovery potential pattern recognition untargeted metabolomics analysis; 3) construction steaming-induced network combined desorption electrospray ionization-mass spectrometry imaging (DESI-MSI). Consequently, 542 were characterized from PG/PQ/PN. Steaming at 1–10 h could cause variation, separately 26, 28, 18 found steaming PG, PQ, PN. Steaming-induced mainly involved hydration malonyl group, hydrolysis glycosyl moiety, dehydration C-20. DESI-MSI further revealed spatial distribution marker saponins in cork layer, phloem, xylem, primarily confirmed reactions occurring steaming. Conclusively, established strategy practical processing, which useful control both herbal medicines foods.

Язык: Английский

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Sustainable production of natural products using synthetic biology: Ginsenosides

Journal of Ginseng Research, Год журнала: 2024, Номер 48(2), С. 140 - 148

Опубликована: Янв. 3, 2024

Synthetic biology approaches offer potential for large-scale and sustainable production of natural products with bioactive potency, including ginsenosides, providing a means to produce novel compounds enhanced therapeutic properties. Ginseng, known its non-toxic potent qualities in traditional medicine, has been used various medical needs. Ginseng shown promise antioxidant neuroprotective properties, it as agent boost immunity against infections when together other drugs vaccines. Given the increasing demand ginsenosides challenges associated extraction methods, synthetic holds development therapeutics. In this review, we discuss recent developments microorganism producer engineering ginsenoside microorganisms using approaches.

Язык: Английский

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Structural-Activity Relationship of Rare Ginsenosides from Red Ginseng in the Treatment of Alzheimer’s Disease

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(10), С. 8625 - 8625

Опубликована: Май 11, 2023

Rare ginsenosides are the major components of red ginseng. However, there has been little research into relationship between structure and their anti-inflammatory activity. In this work, BV-2 cells induced by lipopolysaccharide (LPS) or nigericin, activity eight rare ginsenosides, target proteins expression AD were compared. addition, Morris water maze test, HE staining, thioflavins urine metabonomics used to evaluate effect Rh4 on mice. Our results showed that configuration influences ginsenosides. Ginsenosides Rk1, Rg5, Rk3, have significant compared S-Rh1, R-Rh1, S-Rg3, R-Rg3. S-Rh1 S-Rg3 more pronounced than R-Rh1 R-Rg3, respectively. Furthermore, two pairs stereoisomeric can significantly reduce level NLRP3, caspase-1, ASC in cells. Interestingly, improve learning ability mice, cognitive impairment, hippocampal neuronal apoptosis Aβ deposition, regulate AD-related pathways such as tricarboxylic acid cycle sphingolipid metabolism. findings conclude with a double bond those without, 20(S)-ginsenosides excellent 20(R)-ginsenosides.

Язык: Английский

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Optimizing the extraction process of total saponins from Panax vietnamensis based on response surface analysis and backpropagation neural network-genetic algorithm

Industrial Crops and Products, Год журнала: 2024, Номер 220, С. 119252 - 119252

Опубликована: Июль 18, 2024

Язык: Английский

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Interpreting the efficacy enhancement mechanism of Chinese medicine processing from a biopharmaceutic perspective

Chinese Medicine, Год журнала: 2024, Номер 19(1)

Опубликована: Янв. 18, 2024

Abstract Chinese medicine processing (CMP) is a unique pharmaceutical technology that distinguishes it from natural medicines. Current research primarily focuses on changes in chemical components to understand the mechanisms behind efficacy enhancement processing. However, this paper presents novel perspective biopharmaceutics of CMP. It provides comprehensive overview current research, emphasizing two crucial aspects: role ‘heat’ during and utilization adjuvants. The highlights generation easily absorbed through hydrolysis glycosides by ‘heat’, as well facilitation dissolution, absorption, targeted distribution active From biopharmaceutic perspective, lucid comprehension scientific foundation for augmenting Moreover, proposes three-dimensional framework encompassing reactions, phase transitions, biopharmaceutical properties further investigate involved enhancing Graphical

Язык: Английский

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Increasing the Amounts of Bioactive Components in American Ginseng (Panax quinquefolium L.) Leaves Using Far-Infrared Irradiation

Foods, Год журнала: 2024, Номер 13(4), С. 607 - 607

Опубликована: Фев. 17, 2024

Both the roots and leaves of American ginseng contain ginsenosides polyphenols. The impact thermal processing on enhancing biological activities root by altering its component composition has been widely reported. However, effects far-infrared irradiation (FIR), an efficient heat treatment method, bioactive components remain to be elucidated. In present study, we investigated FIR between 160 200 °C deglycosylation dehydration rates in leaves. As temperature was increased, amounts common decreased while those rare increased. After at optimal 190 °C, highest total polyphenolic content kaempferol were detected, antioxidant activity significantly enhanced, F4, Rg6, Rh4, Rk3, Rk1, Rg3, Rg5 41, 5, 37, 64, 222, 17, 266 times higher than untreated leaves, respectively. Moreover, radical scavenging for 2,2-diphenyl-1-picrylhydrazyl 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) reducing power treated leaf extracts 2.17, 1.86, 1.77 higher, Hence, is method producing beneficial from

Язык: Английский

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Integrated Analysis of Ginsenoside Content and Biomarker Changes in Processed Ginseng: Implications for Anti-Cancer Mechanisms

Foods, Год журнала: 2024, Номер 13(16), С. 2497 - 2497

Опубликована: Авг. 8, 2024

Black ginseng is the processed product of ginseng, and it has been found that content types rare ginsenosides increased after processing. However, there limited research on ginsenoside differences between cultivated forest before processing among various plant parts. This study investigated effects in different parts ginseng. After processing, contents Re, Rg1, S-Rg3, Rg5, R-Rh1, Rk1, Rk3, F4 were significantly or decreased, growth age was not proportional to ginsenosides, from cultivation methods relatively small. Chemometric analysis identified biomarkers showing varying percentage changes Network pharmacology predicted EGFR/PI3K/Akt/mTOR pathway as a potential key for anti-cancer effect black

Язык: Английский

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DGS-YOLOv8: A Method for Ginseng Appearance Quality Detection

Agriculture, Год журнала: 2024, Номер 14(8), С. 1353 - 1353

Опубликована: Авг. 13, 2024

In recent years, the research and application of ginseng, a famous valuable medicinal herb, has received extensive attention at home abroad. However, with gradual increase in demand for discrepancies are inevitable when using traditional manual method grading appearance quality ginseng. Addressing these challenges was primary focus this study. This study obtained batch ginseng samples enhanced dataset by data augmentation, based on which we refined YOLOv8 network three key dimensions: firstly, used C2f-DCNv2 module SimAM mechanism to augment model’s effectiveness recognizing features, followed use Slim-Neck combination (GSConv + VoVGSCSP) lighten model These improvements constitute our proposed DGS-YOLOv8 model, achieved an impressive mAP50 95.3% detection. The improved not only reduced number parameters smaller size but also improves 6.86%, 2.73%, 3.82% precision, mAP50, mAP50-95 over YOLOv8n comprehensively outperforms other related models. With its potential demonstrated experiment, technology can be deployed large-scale production lines benefit food Chinese medicine industries. summary, advantages high detection accuracy, small space occupation, easy deployment, robustness.

Язык: Английский

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Foods for Sleep Improvement: A Review of the Potential and Mechanisms Involved

Foods, Год журнала: 2025, Номер 14(7), С. 1080 - 1080

Опубликована: Март 21, 2025

Insomnia affects one-third of the world’s population; negative effects insomnia are significant, and traditional medications have numerous side cause considerable suffering. This has aroused interest in obtaining sleep-improving substances from foods. study conducted a comprehensive literature review using Web Science PubMed with keywords like “sleep”, “insomnia”, “food”. A subsequent summary revealed that certain foods, including milk, Ziziphus jujuba, Lactuca sativa, ginseng, Schisandra chinensis, Juglans regia, etc., purported to enhance sleep quality by prolonging duration, reducing latency, alleviating anxiety. The mechanisms these foods’ mainly occur via central nervous system, particularly gamma-aminobutyric acid (GABA)ergic 5-hydroxytryptamine (5-HT)ergic systems. Although this supports fact they potential, further research is needed. There also issues such as more limited fewer mechanisms, pharmacokinetic studies, models being involved. These need be addressed future adequately address problem insomnia. It hoped will contribute into foods properties and, future, provide an effective natural alternative for those seeking medication.

Язык: Английский

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MicroRNA-mediated regulation of ginsenoside biosynthesis in Panax ginseng and its biotechnological implications

Science Progress, Год журнала: 2025, Номер 108(2)

Опубликована: Март 31, 2025

Panax ginseng , renowned for its therapeutic properties, derives much of medicinal value from ginsenosides, a group bioactive triterpenoid saponins. The biosynthesis ginsenosides is regulated by various mechanisms, including microRNAs (miRNAs), which play key roles in gene regulation. Recent studies have identified numerous miRNAs P . and other plants, highlighting their potential to influence targeting genes the pathway. This mini-review explores current understanding miRNA-mediated regulation discusses controlling ginsenoside production through miRNA manipulation. Although research still primitive, ongoing suggest promising applications agriculture medicine. Further functional on these could provide valuable insights into optimizing enhancing properties.

Язык: Английский

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