HLF and PPARα axis regulates metabolic‐associated fatty liver disease through extracellular vesicles derived from the intestinal microbiota
iMeta,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 7, 2025
Metabolic-associated
fatty
liver
disease
(MAFLD)
has
become
increasingly
widespread.
The
intestine
is
the
primary
site
of
lipid
absorption
and
important
for
homeostasis
metabolism.
However,
mechanism
underlying
participation
intestinal
tract
in
development
MAFLD
requires
additional
investigation.
In
this
study,
analysis
single-cell
transcriptome
tissue
from
cynomolgus
monkeys
found
that
hepatic
leukemia
factor
(HLF)
participated
genetic
regulation
absorption.
Results
obtained
normal
intestine-specific
Hlf-knockout
mice
confirmed
HLF
alleviated
barrier
disorders
by
inhibiting
peroxisome
proliferator-activated
receptor
alpha
(PPARα)
expression.
HLF/PPARα
axis
mediating
gut
microbiota-derived
extracellular
vesicles
(fEVs),
thereby
hepatocyte
ferroptosis.
Lipidomics
functional
experiments
verified
taurochenodeoxycholic
acid
(TCDCA),
a
conjugated
bile
contained
fEVs,
had
key
role
process.
conclusion,
activity
was
mediated
fEVs
identified
as
novel
therapeutic
target
MAFLD.
Язык: Английский
An Optimized Aerogel‐Based Apheresis Device for Targeted Lipid Clearance in Elderly Hyperlipidemia Patients
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 21, 2025
Abstract
Elderly
patients
with
hyperlipidemia
often
exhibit
resistance
to
conventional
hypolipidemic
treatments,
underscoring
the
need
for
more
effective
strategies
address
lipid
imbalances
in
this
high‐risk
group.
This
study
introduces
LipClean,
an
aerogel‐based
apheresis
device
specifically
designed
remove
harmful
plasma
lipids.
LipClean
is
constructed
using
hydrophilic
cellulose
fibers,
which
serve
as
a
supramolecular
platform
synthesizing
hydrophobic
conjugated
polymers
through
Sonogashira‐Hagihara
reaction.
These
are
then
cross‐linked
fibers
via
phosphorylation,
generating
aerogel
monolith
interpenetrating
network
of
and
polymers.
Unlike
bilayer
aerogels
that
separate
layers,
LipClean's
structure
precisely
engineered
polymer
design
gradient
cross‐linking.
optimization
enhances
both
bodily
fluid
flow
adsorption
while
minimizing
removal
essential
components
ensuring
unobstructed
cell
passage.
In
preclinical
testing,
significantly
reduced
triglyceride
cholesterol
levels
elderly
rat
model
normalized
blood
samples
from
hypertensive
patients.
Importantly,
purified
maintained
normal
cells
physiological
biochemical
indicators
after
apheresis,
highlighting
potential
managing
hyperlipidemia‐related
disorders.
study,
therefore,
underscores
importance
interdisciplinary
collaboration
driving
medical
innovation.
Язык: Английский
Effects of Hawthorn Flavonoids on Intestinal Microbial Community and Metabolic Phenotype in Obese Rats
Yu Bi,
Chang Lv,
Juanjuan Zhu
и другие.
Advanced Biology,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 12, 2024
Abstract
Obesity
(OB)
is
a
prevalent
metabolic
disorder.
With
the
advancement
of
economy,
prevention
and
treatment
obesity
big
problem
for
global
community.
The
methods
to
lose
weight
include
exercise,
diet,
medicine,
surgery.
Compared
with
other
methods,
diet
regulation
safer
more
effective.
Hawthorn
fruit
has
effect
reducing
weight,
but
mechanism
effectiveness
are
not
clear.
In
this
study,
model
rats
used
conduct
scientific
pharmacological
research
on
hawthorn
flavonoids.
flavonoids
can
effectively
improve
body
lipid
accumulation,
levels
obese
rats.
contents
colon
analyzed
using
16S
rDNA
sequencing
technology.
intestinal
microflora
in
changed
significantly
after
treatment,
they
tended
be
control
group.
Based
liquid
chromatography‐mass
spectrometry,
serum
metabolomics
showed
that
metabolites
significantly,
treatment.
especially
involved
biological
processes
grade
bile
acid
biosynthesis,
histidine
metabolism,
metabolism.
Pearson
correlation
analysis
disorder
microorganisms
connected
changes
metabolites.
These
findings
give
new
idea
about
how
help
obesity.
Язык: Английский
Chronic lead poisoning–induced budgerigar liver damage, gut microbiota dysbiosis, and metabolic disorder
Chongyang Yu,
Na Xu,
Xiaoyu Tao
и другие.
Ecotoxicology and Environmental Safety,
Год журнала:
2024,
Номер
278, С. 116388 - 116388
Опубликована: Май 3, 2024
Birds
are
sensitive
to
heavy
metal
pollution,
and
lead
(Pb)
contamination
can
negatively
affect
their
liver
gut.
Therefore,
we
used
budgerigars
examine
gut
toxicosis
caused
by
Pb
exposure
in
bird,
the
possible
toxic
mechanisms.
The
findings
showed
increased
weight
decreased
body
weight.
Moreover,
histopathological
immunofluorescence
assay
results
demonstrated
obvious
damage
cell
apoptosis
Pb-
treated
budgerigars.
Quantitative
polymerase
chain
reaction
(qPCR)
also
an
increase
inhibiting
PPAR-γ/PI3K/Akt
pathway.
microbe
analyses
indicated
Firmicutes,
Proteobacteria,
Bacteroidetes
were
dominant
microbial
phyla,
Network
analysis
shown
Arthrobacter,
Bradyrhizobium
Alloprevotella
as
hubs
of
Modules
I,
II,
III,
respectively.
Phenylpropanoids
polyketides,
Organoheterocyclic
compounds,
Organic
oxygen
nitrogen
compounds
metabolite
superclasses.
Tauroursodeoxycholic
acid,
taurochenodeoxycholic
acid
(sodium
salt),
2-[2-(5-bromo-2-pyridyl)diaz-1-enyl]-5-(diethylamino)phenol
significantly
enriched
Pb-treated
group.
It
that
41
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
orthologues
183
pathways
differed
between
control
using
metabolomic
data.
orthogonal
partial
least-squares
discrimination
(OPLS-DA)
based
on
distinct
clusters
groups.
Additionally,
correlation
a
positive
for
groups
microbiota
data,
Furthermore,
microenvironment
found
each
other,
this
study
especially
may
pose
serious
health
risks
birds
through
"gut-liver
axis"
too.
Язык: Английский
Fuel stores and time of day account for variation in serum metabolomes of passerine migrants stopping over
Journal of Avian Biology,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 12, 2024
Migratory
birds
excel
in
phenotypic
flexibility,
adapting
physiologically
as
their
life
histories
and
environments
require.
Discerning
the
metabolic
processes
underlying
migrants'
physiology,
an
emergent
property
of
multiple
continuous
dynamic
organism–environment
interactions,
is
therefore
challenging,
particularly
under
natural
conditions.
Accordingly,
analyses
snapshot‐sampled
serum‐circulating
metabolites,
versatile
readily
applicable
for
migrating
birds,
have
increasingly
become
method
choice
such
physiologic
inference.
However,
atemporal
nature
single
sampling
might
obscure
links
between
observed
metabolite
concentrations
producing
them,
necessitating
analytical
decoupling
focal
from
broader
biochemical
background.
In
present
study,
we
examined
how
variation
combined
fat
muscle
fuel
stores,
traits
pivotal
migratory
context,
relates
to
metabolomes
spring‐migrating
Eurasian
blackcaps
stopping‐over.
Our
accounted
potential
spatiotemporal
influences
form
time
past
night's
fasting
random
local
conditions
across
three
sites
within
Negev
Desert.
We
shifted
focus
compound‐level
analysis
preselected
metabolites
towards
level
inclusive
metabolome,
quantifying
lipophilic
polar
molecules
via
UHPLC–MS/MS
untargeted
metabolomic
technique.
results
indicated
a
general
relationship
stores
comprising
16
326
6923
compounds,
among
which
918
44
were
annotated,
respectively.
By
applying
generalized
latent‐variable
linear
modeling
(GLLVM)
upon
annotated
identified
several
candidate
biomarkers,
some
novel
notably
fuel‐associated
increase
serum
ceramides
likely
derived
circulating
very
low‐density
lipoproteins
(VLDLs).
Relying
on
estimated
with
foraging
modeled
residual
covariations
demonstrate
fuel–metabolite
associations
generally
consistent
higher
fat‐
lower
protein
mobilization
having
greater
decreased
utilization
ingested
nutrients
accumulate
over
time,
thus
introducing
approach
physiological
study
birds.
Язык: Английский
Intrauterine exposure to long-chain perfluorocarboxylic acids (PFCAs) were associated with reduced primary bile acids in three-year-old children: Findings from a prospective birth cohort study
Environmental Pollution,
Год журнала:
2024,
Номер
360, С. 124583 - 124583
Опубликована: Июль 20, 2024
Язык: Английский
Lipidomic analysis to reveals therapeutic effects of herbal cake-separated moxibustion on high-fat diet-induced hyperlipidemia rabbits
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 8, 2024
Abstract
Background
Herbal
cake-separated
moxibustion
(HM)
is
one
of
the
characteristic
therapies
for
prevention
and
treatment
hyperlipidemia
(HLP).
However,
effect
HM
on
plasma
lipid
metabolism
in
HLP
rabbits
not
clear.
Methods
New
Zealand
were
fed
with
high-fat
diet
8
weeks
to
induce
model,
then
was
intervened
weeks.
The
level
blood
serum
detected
by
full
biochemical
analyzer,
pathological
changes
liver
tissue
observed
oil
red
O
staining.
Then
we
used
ultra-high
performance
liquid
chromatography
/
quadrupole
time-of-flight
mass
spectrometry
combined
multivariate
statistical
analysis
non-targeted
lipidomic
analysis.
Results
ameliorated
induced
abnormal
improved
deposition
high
cholesterol
diet.
Non-targeted
showed
that
changed
profile
rabbits.
Язык: Английский