Abstract
Cancer
remains
a
significant
global
health
challenge
characterized
by
the
uncontrolled
proliferation
of
malignant
cells.
While
conventional
treatments
such
as
chemotherapy
and
radiotherapy
are
effective,
they
often
cause
severe
side
effects,
driving
interest
in
alternative
therapies
derived
from
medicinal
plants.
These
plants
rich
bioactive
compounds,
including
terpenoids,
phenolics,
flavonoids,
which
exhibit
promising
anticancer
properties.
This
review
focuses
on
gas
chromatography–mass
spectrometry
(GC–MS)
for
identifying
characterizing
these
phytochemicals,
highlighting
its
value
natural
product
research
due
to
precise
separation
identification
capabilities.
An
analysis
over
30
studies
identified
key
compounds
with
cytotoxic
effects.
Limonene
(IC
50
:
47–57.34
µg/mL
A549,
HepG2,
CaCo,
PANC‐1
cells),
squalene
26.22
JURKAT),
sesamin
52–57.2
MCF‐7
HCT116
β
‐caryophyllene
19.4–58.2
ME‐180
cells)
have
exhibited
potent
activities.
Despite
advantages,
GC‐MS
realizes
limitations,
difficulty
differentiating
structural
isomers
detecting
low‐abundance
compounds.
Integrating
GC–MS
complementary
analytical
techniques
can
overcome
challenges
enhance
phytochemical
profiling.
Addressing
bioavailability
conducting
clinical
trials
is
essential
translating
findings
into
effective
cancer
therapies.
underscore
therapeutic
potential
phytochemicals
safer
alternatives
treatments.
Frontiers in Drug Discovery,
Год журнала:
2024,
Номер
4
Опубликована: Июль 17, 2024
Antimicrobial
Resistance
(AMR)
is
a
critical
global
health
challenge,
and
in
this
review
article,
we
examine
the
limitations
of
traditional
therapeutic
methods
emerging
role
alternative
therapies.
By
examining
reasons
behind
failure
conventional
treatments,
including
inadequacy
one-drug-one-enzyme
approaches,
complex
evolution
AMR,
impact
drug
biotransformation,
better
understand
why
treatments
failed.
Moreover,
discusses
several
therapies,
RNA-based
aptamers,
peptide-based
phage
therapy,
probiotics,
discussing
their
applications,
advantages,
limitations.
Additionally,
discuss
obstacles
to
develop
these
funding
shortages,
regulatory
barriers,
public
perception.
This
comprehensive
analysis
aims
provide
insight
into
future
emphasizing
need
for
innovative
strategies
practical
approaches.
Muscular
dystrophies
(MDs)
are
an
array
of
inherited
disorders
characterized
by
progressive
muscle
weakness
and
degeneration.
This
review
explores
the
genetic
foundation,
pathophysiological
mechanisms,
current
treatments,
therapeutic
potential
medicinal
plants
for
MDs.
Although
gene
therapy,
exon-skipping,
stem
cell
therapies
substantial
advancements,
they
encounter
obstacles
regarding
immune
responses,
long-term
efficacy,
delivery
efficiency.
A
promising
complementary
approach
to
conventional
MD
is
presented
natural
compounds
from
plants.
Recent
studies
historical
use
underscore
multi-targeted
effects
phytochemicals,
such
as
resveratrol,
withaferin
A,
ginsenosides,
curcumin,
epigallocatechin
gallate,
on
regeneration,
oxidative
stress,
inflammation.
These
have
demonstrated
in
pre-clinical
enhance
function,
reduce
inflammation,
stress.
Despite
their
potential,
utilization
treatment
impeded
regulatory
hurdles,
side
effects,
variability
bioactive
compound
concentration,
standardization
issues.
To
address
these
challenges,
it
necessary
implement
rigorous
clinical
trials,
protocols,
comprehensive
quality
control
measures.
Future
research
should
focus
elucidating
molecular
mechanisms
plant-derived
compounds,
improving
biotechnological
production
techniques,
integrating
treatments
into
personalized,
plans.
Advances
synthetic
biology,
genomics,
metabolomics,
nanoformulations
improve
efficacy
safety
phytomedicine
MD.
Implementing
evidence-based
integrative
approaches
can
holistic
care,
patient
outcomes,
facilitate
acceptance
plant-based
practice.
Advances in Human Biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 3, 2025
Abstract
Introduction:
Root
canal
treatment
involves
eliminating
microorganisms
and
necrotic
tissue
through
biomechanical
debridement
root
filling
to
prevent
reinfection.
Disinfection
is
enhanced
by
removing
the
smear
layer,
composed
of
organic
inorganic
debris,
using
irritants
like
sodium
hypochlorite
(NaOCl)
for
dissolution
ethylenediaminetetraacetic
acid
(EDTA)
chelation.
Despite
their
efficacy,
these
agents
have
limitations,
such
as
cytotoxicity
dentinal
erosion.
Research
into
biocompatible
alternatives,
including
herbal
extracts
essential
oils,
shows
promise
safer
more
effective
disinfection.
Materials
Methods:
Eighty
extracted
single-rooted
premolars
were
studied
pulp
layer
removal.
Neem
leaf
(Group
A),
orange
peel
B)
grape
seed
C)
tested
against
3%
NaOCl
D)
17%
EDTA
E)
Pulp
was
assessed
weighing
specimens
before
after
immersion
in
solutions,
while
removal
evaluated
scanning
electron
microscopy.
Results
statistically
analysed.
Results:
On
statistical
analysis,
showed
significantly
higher
compared
experimental
extract
at
all-time
intervals,
i.e.
30,
60
90
min.
intergroup
comparison
scores,
produced
with
a
mean
score
0.4
other
extracts.
Conclusion:
Herbal
exhibited
no
significant
min
NaOCl.
Similarly,
none
comparable
efficacy.
However,
amongst
extracts,
better
than
Orange
extract.
