Participação dos nitrosotiois nos efeitos do nitrito de sódio sobre as gelatinases vasculares >i<in vitro>/i< e >i<in vivo>/i< DOI Creative Commons

Rose I. M. Batista

Опубликована: Апрель 16, 2024

Matrix metalloproteinases (MMPs) are a group of endopeptidases that participate on the extracellular matrix (ECM) degradation, resulting in vascular remodeling.In hypertension, sodium nitrite treatment reduces MMP-2 activity through mechanisms have not been elucidated yet.Thus, objective this study was to evaluate whether beneficial effects arterial hypertension due production nitrosothiols and MMPs nitrosylation, decrease their activity.For this, we used smooth muscle cells (VSMCs) treated with PMA, PMA+nitrite, PMA+GSNO, Ang II, II+nitrite, AngII+GSNO, control groups.Gelatinases measured by situ zymography.We also performed treatments RNAi for GSNO reductase (GSNOR) elucidate participation anti-MMP nitrite.In vivo part work, Sham hypertensive rats (2 kidney-oneclip-model) vehicle, (1mg/kg orally) SPL-334 (0,3mg/kg IP, GSNOR inhibitor).We blood pressure these animals weekly tail plethysmography, plasmatic nitrite, total nitrosylated species levels, as well aortic levels were an ozone-based chemiluminescence assay.We morphometric parameters assess remodeling from animals.In addition, 2K1C (15mg/kg nitrite+omeprazole (10mg/kg intraperitoneally).We systolic pressure, stomach pH, oxidative stress using lucigenin DHE methods, gelatinases gelatin zymography remodeling.As results, observed cell PMA II increased whereas able reverse increasement, meanwhile had no effects.RNAi expression increase nitrosylation.In where activator MMPs, it effect only RNAi.However, angiotensin both groups (control GSNOR-), inhibited activity.In animals, generated more pronounced than when drugs separately, especially fourth week protocol.We concentration after SPL 334.Treatments SPL-344 alone association media/lumen (M/L) parameter.However, concerning cross-section area (CSA) parameter, two cause effects.Regarding animal protocol omeprazole 15 mg/kg, reduce weeks 3, 5 6 tended hypertension.Nitrite superoxide NADPH oxidase reversed effects.A similar activity, nitrite.Regarding zymography, there 64 72 kDa did any changes.Omeprazole CSA parameter remodeling.These results suggest over would be, at least part, related formation stomach, which lead decreasing its consequence.

Язык: Английский

Fabrication and application of gold nanoparticles functionalized polymer monolith in spin column for the determination of S-nitrosoglutathione in meat DOI

Shengman Zhang,

Wenxiu Zhou,

Xiaoyuan Lv

и другие.

Food Chemistry, Год журнала: 2024, Номер unknown, С. 141210 - 141210

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

1
Recombinant hirudin attenuates pulmonary hypertension and thrombosis in acute pulmonary embolism rat model DOI Creative Commons

Xiang Wei,

Yanfen Zou,

Shunli Dong

и другие.

PeerJ, Год журнала: 2024, Номер 12, С. e17039 - e17039

Опубликована: Апрель 5, 2024

Background Acute pulmonary embolism (APE) is classified as a subset of diseases that are characterized by lung obstruction due to various types emboli. Current clinical APE treatment using anticoagulants frequently accompanied high risk bleeding complications. Recombinant hirudin (R-hirudin) has been found have antithrombotic properties. However, the specific impact R-hirudin on remains unknown. Methods Sprague-Dawley (SD) rats were randomly assigned five groups, with thrombi injections establish models. Control and group subcutaneously injected equal amounts dimethyl sulfoxide (DMSO). The APE+R-hirudin low-dose, middle-dose, high-dose groups received subcutaneous at doses 0.25 mg/kg, 0.5 1.0 respectively. Each was subdivided into time points 2 h, 6 1 d, 4 animals per point. Subsequently, all euthanized, serum tissues collected. Following assessment right ventricular pressure (RVP) mean artery (mPAP), blood gas analysis, enzyme-linked immunosorbnent assay (ELISA), vascular testing, hematoxylin-eosin (HE) staining, Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) immunohistochemistry, Western blot experiments conducted. Results caused significant reduction mPAP, RVP, Malondialdehyde (MDA) content, well H O myeloperoxidase (MPO) activity, while increasing oxygen (PaO ) Superoxide Dismutase (SOD) activity. also decreased wall area ratio thickness diameter in rat arteries. Serum levels endothelin-1 (ET-1) thromboxaneB2 (TXB2) decreased, prostaglandin (6-K-PGF1 α NO increased. Moreover, ameliorated histopathological injuries reduced apoptotic cells Matrix metalloproteinase-9 (MMP9), cell adhesion molecule-1 (VCAM-1), p-Extracellular signal-regulated kinase (ERK)1/2/ERK1/2, p-P65/P65 expression tissues. Conclusion attenuated hypertension thrombosis rats, suggesting its potential novel strategy for APE.

Язык: Английский

Процитировано

0
Participação dos nitrosotiois nos efeitos do nitrito de sódio sobre as gelatinases vasculares >i<in vitro>/i< e >i<in vivo>/i< DOI Creative Commons

Rose I. M. Batista

Опубликована: Апрель 16, 2024

Matrix metalloproteinases (MMPs) are a group of endopeptidases that participate on the extracellular matrix (ECM) degradation, resulting in vascular remodeling.In hypertension, sodium nitrite treatment reduces MMP-2 activity through mechanisms have not been elucidated yet.Thus, objective this study was to evaluate whether beneficial effects arterial hypertension due production nitrosothiols and MMPs nitrosylation, decrease their activity.For this, we used smooth muscle cells (VSMCs) treated with PMA, PMA+nitrite, PMA+GSNO, Ang II, II+nitrite, AngII+GSNO, control groups.Gelatinases measured by situ zymography.We also performed treatments RNAi for GSNO reductase (GSNOR) elucidate participation anti-MMP nitrite.In vivo part work, Sham hypertensive rats (2 kidney-oneclip-model) vehicle, (1mg/kg orally) SPL-334 (0,3mg/kg IP, GSNOR inhibitor).We blood pressure these animals weekly tail plethysmography, plasmatic nitrite, total nitrosylated species levels, as well aortic levels were an ozone-based chemiluminescence assay.We morphometric parameters assess remodeling from animals.In addition, 2K1C (15mg/kg nitrite+omeprazole (10mg/kg intraperitoneally).We systolic pressure, stomach pH, oxidative stress using lucigenin DHE methods, gelatinases gelatin zymography remodeling.As results, observed cell PMA II increased whereas able reverse increasement, meanwhile had no effects.RNAi expression increase nitrosylation.In where activator MMPs, it effect only RNAi.However, angiotensin both groups (control GSNOR-), inhibited activity.In animals, generated more pronounced than when drugs separately, especially fourth week protocol.We concentration after SPL 334.Treatments SPL-344 alone association media/lumen (M/L) parameter.However, concerning cross-section area (CSA) parameter, two cause effects.Regarding animal protocol omeprazole 15 mg/kg, reduce weeks 3, 5 6 tended hypertension.Nitrite superoxide NADPH oxidase reversed effects.A similar activity, nitrite.Regarding zymography, there 64 72 kDa did any changes.Omeprazole CSA parameter remodeling.These results suggest over would be, at least part, related formation stomach, which lead decreasing its consequence.

Язык: Английский

Процитировано

0