Improvement of antioxidant protection and energy production in neurodegenerative processes in the brain caused by long-term alcoholism under the influence of Lophantus chinensis Benth extract

Сибирский научный медицинский журнал, Год журнала: 2025, Номер 44(6), С. 171 - 178

Опубликована: Янв. 8, 2025

Long-term alcohol abuse causes psycho-emotional and cognitive impairment, including severe dementia. Oxidative stress is considered one of the main mechanisms in disorders pathophysiology caused by long-term alcoholism. In this regard, search for substances capable correcting mitochondrial dysfunction oxidative that arise as a result prolonged ethanol consumption relevant. Of particular interest complex treatment alcoholic encephalopathy Chinese hyssop ( Lophanthus chinensis ), used Tibetan Mongolian traditional medicine liver diseases, well means improving functional state body metabolism, slowing down aging process. Material methods . Alcohol intoxication was modeled Wistar rats per os administration 40 % solution volume 10 ml/kg six weeks. The L. dry extract at dose 100 mg/kg administered to animals starting from third week, an hour after ethanol. On day 45, content malondialdehyde (MDA), reduced glutathione (GSH), activity catalase, superoxide dismutase (SOD), peroxidase (GPx), reductase, NADH dehydrogenase succinate complex, concentration ATP werei determined spectrophotometrically animal brain homogenate. Results It found increases dismutase, peroxidase, reductase (by %, р = 0.016, 31 0.001, 30 0.041, 29 0.009, respectively) GSH 24 0.019), also reduces MDA 20 0.014) tissue. A decrease intensity enhances functioning enzymatic complexes I 23 0.017) II respiratory chain 72 0.001) 0.029) rat’s brain. Conclusions exhibits antioxidant effects stimulates energy processes neurodegenerative

Язык: Английский

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Scopoletin as a Cardioprotective Agent Against Cisplatin-Induced Oxidative Stress and Inflammation

Phytomedicine Plus, Год журнала: 2025, Номер unknown, С. 100738 - 100738

Опубликована: Янв. 1, 2025

Язык: Английский

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Scopoletin alleviates acetaminophen-induced hepatotoxicity through modulation of NLRP3 inflammasome activation and Nrf2/HMGB1/TLR4/NF-κB signaling pathway

International Immunopharmacology, Год журнала: 2025, Номер 148, С. 114132 - 114132

Опубликована: Янв. 26, 2025

Язык: Английский

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A validated HPTLC method for quantification of active compounds in a Polyherbal formulation Guggulutiktam Kashayam

Vegetos, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Язык: Английский

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Biochemical Screening of Phytochemicals and Identification of Scopoletin as a Potential Inhibitor of SARS-CoV-2 Mpro, Revealing Its Biophysical Impact on Structural Stability

Viruses, Год журнала: 2025, Номер 17(3), С. 402 - 402

Опубликована: Март 12, 2025

The main protease (Mpro or 3CLpro nsp5) of SARS-CoV-2 is crucial to the life cycle and pathogenesis SARS-CoV-2, making it an attractive drug target develop antivirals. This study employed virtual screening a few phytochemicals, resultant best compound, Scopoletin, was further investigated by FRET-based enzymatic assay, revealing experimental IC50 15.75 µM. impact Scopoletin on Mpro biophysical MD simulation studies. Fluorescence spectroscopy identified strong binding constant 3.17 × 104 M⁻1 for Mpro, as demonstrated its effective fluorescence quenching Mpro. Additionally, CD showed significant reduction in helical content upon interaction with Scopoletin. findings thermodynamic measurements using isothermal titration calorimetry (ITC) supported spectroscopic data, indicating tight KA 2.36 103 M−1. Similarly, studies have also revealed that forms hydrogen bonds amino acids nearest active site, this has been molecular dynamics These indicate may be developed potential antiviral treatment targeting

Язык: Английский

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Wild grown Portulaca oleracea as a novel magnetite based carrier with in vitro antioxidant and cytotoxicity potential

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 13, 2025

The latest research on nanotechnology through the new tailored scaffolds encompassed therapeutic effects of natural compounds, and unique properties metallic nanoparticles offer possibilities in emerging biomedical fields. Various strategies have been developed to address limitations existing agents concerning specificity, vectorization, bioavailability, drug resistance, adverse effects. In this study, medicinal plant Portulaca oleracea L. magnetite were used develop an innovative target carrier system, designed enhance cytotoxic effect overcome main drawbacks (permeability localization) phytoconstituents. low-metabolite profile Romanian wild-grown exhibits a diverse range hundred fifty-five compounds across various chemical categories (amino acids, peptides, fatty flavonoids, alkaloids, terpenoids, phenolic organic esters, sterols, coumarins, nucleosides, lignans, miscellaneous compounds). Morpho-structural magnetic phytocarrier investigated using variety methods, including XRD, FTIR, Raman, SEM, DLS), determinations. MTT assay was conducted evaluate vitro potential cytotoxicity normal human dermal fibroblasts (NHDF), as well two tumoral cell lines: osteosarcoma (HOS) cervical cancer (HeLa). Results indicated that significant inhibition both lines' viability exerted by compared herbal extract. Furthermore, results obtained for total content antioxidant screening performed FRAP DPPH assays superior system. These findings suggest applications system its promising implications future development field.

Язык: Английский

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Influence of Plant Part Selection and Drying Technique: Exploration and Optimization of Antioxidant and Antibacterial Activities of New Guinea Impatiens Extracts

Plants, Год журнала: 2025, Номер 14(7), С. 1092 - 1092

Опубликована: Апрель 1, 2025

Impatiens L. plants are sources of polyphenols with antioxidant and antimicrobial activities. There scarce data about these effects in the case hawkeri W. Bull, a relevant species ornamental plant industry ethnobotanical backgrounds. The aim this study is to provide information regarding antibacterial properties ethanol extracts I. support new applications. HPTLC was used estimate concentration seven known bioactive metabolites reported among plants. Total phenolics, flavonoids, monomeric anthocyanins were also measured. An orthogonal platform chemical biological vitro assays evaluate activity extracts. Antibacterial determined by broth microdilution assay on human pathogenic bacteria. results integrated correlation principal component analysis identify most promissory part drying technique optimize evaluated Data suggest tentative identification markers for activities (quercetin rutin). Freeze-dried leaves flowers parts development nutraceuticals or preservatives. demonstrate that phenolic compounds play major role hawkery

Язык: Английский

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Insights on the Phytochemistry and Bioactive Properties of Ailanthus altissima (Mill.) Swingle for Nutritional and Curative Potential

Current Pharmacology Reports, Год журнала: 2025, Номер 11(1)

Опубликована: Апрель 1, 2025

Язык: Английский

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Methanolic extract of Merremia emarginata (Burm. f.) Hallier f. attenuates renal damage in streptozotocin-induced diabetic nephropathy in rats

Obesity Medicine, Год журнала: 2024, Номер 48, С. 100544 - 100544

Опубликована: Апрель 26, 2024

Язык: Английский

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Genes to specialized metabolites: accumulation of scopoletin, umbelliferone and their glycosides in natural populations of Arabidopsis thaliana

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Авг. 27, 2024

Abstract Background Scopoletin and umbelliferone belong to coumarins, which are plant specialized metabolites with potent wide biological activities, the accumulation of is induced by various environmental stresses. Coumarins have been detected in species, including medicinal plants model organism Arabidopsis thaliana . In recent years, key role coumarins maintaining iron (Fe) homeostasis has demonstrated, as well their significant impact on rhizosphere microbiome through exudates secreted into soil environment. Several mechanisms underlying these processes require clarification. Previously, we demonstrated that an excellent for studying genetic variation molecular basis coumarin plants. Results Here, targeted metabolic profiling gene expression analysis, gene-metabolite network scopoletin was examined more detail selected accessions (Col-0, Est-1, Tsu-1) undergoing different culture conditions characterized content. The highest roots grown vitro liquid culture. 10 phenylpropanoid genes ( 4CL1 , 4CL2 4CL3 CCoAOMT1 C3’H HCT F6’H1 F6’H2 CCR1 CCR2 ) assessed qPCR three backgrounds, cultured soil, two types tissues (leaves roots). We not only expected variability among accessions, but also found interesting polymorphisms coding sequences silico analysis resequencing. Conclusions To best our knowledge, this first study comparing simple phenylpropanoid-related cultures. large variations content genetically determined, tissue dependent. It particularly important considering growing media a widely used technology provides amount root suitable metabolomics. Research differential related will be useful future studies aimed at better understanding physiological surrounding environments.

Язык: Английский

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