Identification and Characterization of Two Aryl Sulfotransferases from Deep-Sea Marine Fungi and Their Implications in the Sulfation of Secondary Metabolites DOI Creative Commons

Nicolas Graziano,

Beatriz Arce-López, Tristan Barbeyron

и другие.

Marine Drugs, Год журнала: 2024, Номер 22(12), С. 572 - 572

Опубликована: Дек. 20, 2024

Sulfation plays a critical role in the biosynthesis of small molecules, regulatory mechanisms such as hormone signaling, and detoxification processes (phase II enzymes). The sulfation reaction is catalyzed by broad family enzymes known sulfotransferases (SULTs), which have been extensively studied animals due to their medical importance, but also plant key processes. Despite identification some sulfated metabolites fungi, underlying fungal remain largely unknown. To address this knowledge gap, we conducted comprehensive search available genomes, resulting 174 putative SULT genes Ascomycota phylum. Phylogenetic analysis structural modeling revealed that these SULTs belong aryl sulfotransferase family, they are divided into two potential distinct clusters PAPS-dependent within kingdom. from marine fungi isolated deep-sea hydrothermal vents, Hortaea werneckii UBOCC-A-208029 (HwSULT) Aspergillus sydowii UBOCC-A-108050 (AsSULT), were selected representatives each cluster. Recombinant proteins expressed Escherichia coli biochemically characterized. HwSULT demonstrated high versatile activity, while AsSULT appeared more substrate-specific. Here, was used sulfate mycotoxin zearalenone, enhancing its cytotoxicity toward healthy feline intestinal cells.

Язык: Английский

Identification and Characterization of Two Aryl Sulfotransferases from Deep-Sea Marine Fungi and Their Implications in the Sulfation of Secondary Metabolites DOI Creative Commons

Nicolas Graziano,

Beatriz Arce-López, Tristan Barbeyron

и другие.

Marine Drugs, Год журнала: 2024, Номер 22(12), С. 572 - 572

Опубликована: Дек. 20, 2024

Sulfation plays a critical role in the biosynthesis of small molecules, regulatory mechanisms such as hormone signaling, and detoxification processes (phase II enzymes). The sulfation reaction is catalyzed by broad family enzymes known sulfotransferases (SULTs), which have been extensively studied animals due to their medical importance, but also plant key processes. Despite identification some sulfated metabolites fungi, underlying fungal remain largely unknown. To address this knowledge gap, we conducted comprehensive search available genomes, resulting 174 putative SULT genes Ascomycota phylum. Phylogenetic analysis structural modeling revealed that these SULTs belong aryl sulfotransferase family, they are divided into two potential distinct clusters PAPS-dependent within kingdom. from marine fungi isolated deep-sea hydrothermal vents, Hortaea werneckii UBOCC-A-208029 (HwSULT) Aspergillus sydowii UBOCC-A-108050 (AsSULT), were selected representatives each cluster. Recombinant proteins expressed Escherichia coli biochemically characterized. HwSULT demonstrated high versatile activity, while AsSULT appeared more substrate-specific. Here, was used sulfate mycotoxin zearalenone, enhancing its cytotoxicity toward healthy feline intestinal cells.

Язык: Английский

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