The Oncolytic Effect of Aerva lanata on Osteosarcoma Cell Lines via the Apoptotic Signaling Pathway DOI Open Access

Ummu Zuvairiya,

S Menaka,

Selvaraj Jayaraman

и другие.

Cureus, Год журнала: 2024, Номер unknown

Опубликована: Апрель 12, 2024

Introduction Osteosarcoma, a malignant bone tumor, poses significant treatment challenges, necessitating the development of alternative therapeutic strategies. Aerva lanata (A. lanata), medicinal plant with traditional use in various healthcare systems, has anti-cancer properties. This study looks at oncolytic effect A. extract on osteosarcoma cell lines (sarcoma osteogenic-Saos2). Aim The aim this was to investigate Saos2 through apoptotic signaling pathway. Materials and methods prepared using Soxhlet extraction, its cytotoxic effects cells were assessed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Real-time polymerase chain reaction (RT-PCR) analysis gene activity used assess extract's pathways. Results MTT assay demonstrated dose-dependent decrease proliferation following concentrations ranging from 50 μg 200 μg. standard deviations observed ranged 1.414 7.071. Gene expression revealed that led reduction messenger ribonucleic acid (mRNA) levels anti-apoptotic marker B-cell lymphoma 2 (Bcl2), 1 0.535. Conversely, it induced an increase mRNA tumor suppressor protein p53, 1.835. These findings suggest modulates pathways Bcl2 p53 genes. Conclusion exhibits promising against lines, inducing apoptosis by downregulating increasing p53. study's may be useful as natural for osteosarcoma.

Язык: Английский

Nano-selenium alleviated immunoresponse, apoptosis and oxidative stress in leydig cells of yak DOI
Hao Li,

Yujun Tang,

Hui Wang

и другие.

Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер unknown, С. 114684 - 114684

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Green Synthesis of Selenium Nanoparticles Utilizing Drimia indica: Insights Into Anticancer and Antimicrobial Activities DOI

Fuad Ameen,

Norah Salem Almalki,

Rawan M. Alshalan

и другие.

Microscopy Research and Technique, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

Selenium nanoparticles (SeNPs) have garnered significant interest as anticancer and antimicrobial agents. The aqueous extract of medicinal plant Drimia indica leaves (DI-LAE) was used to synthesize SeNPs (DI-SeNPs) that were extensively characterized by UV-visible absorbance, TEM, EDX, XRD, zeta potential measurements, FTIR. DI-SeNPs exhibited dose-dependent toxicity against the human lung adenocarcinoma cell line (A549; IC

Язык: Английский

Процитировано

2
The Oncolytic Effect of Aerva lanata on Osteosarcoma Cell Lines via the Apoptotic Signaling Pathway DOI Open Access

Ummu Zuvairiya,

S Menaka,

Selvaraj Jayaraman

и другие.

Cureus, Год журнала: 2024, Номер unknown

Опубликована: Апрель 12, 2024

Introduction Osteosarcoma, a malignant bone tumor, poses significant treatment challenges, necessitating the development of alternative therapeutic strategies. Aerva lanata (A. lanata), medicinal plant with traditional use in various healthcare systems, has anti-cancer properties. This study looks at oncolytic effect A. extract on osteosarcoma cell lines (sarcoma osteogenic-Saos2). Aim The aim this was to investigate Saos2 through apoptotic signaling pathway. Materials and methods prepared using Soxhlet extraction, its cytotoxic effects cells were assessed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Real-time polymerase chain reaction (RT-PCR) analysis gene activity used assess extract's pathways. Results MTT assay demonstrated dose-dependent decrease proliferation following concentrations ranging from 50 μg 200 μg. standard deviations observed ranged 1.414 7.071. Gene expression revealed that led reduction messenger ribonucleic acid (mRNA) levels anti-apoptotic marker B-cell lymphoma 2 (Bcl2), 1 0.535. Conversely, it induced an increase mRNA tumor suppressor protein p53, 1.835. These findings suggest modulates pathways Bcl2 p53 genes. Conclusion exhibits promising against lines, inducing apoptosis by downregulating increasing p53. study's may be useful as natural for osteosarcoma.

Язык: Английский

Процитировано

1