Food Science & Nutrition,
Год журнала:
2024,
Номер
12(12), С. 10716 - 10733
Опубликована: Ноя. 24, 2024
ABSTRACT
The
current
study
aims
to
prepare
a
green
extract
using
new
method
in
addition
conventional
extraction
methods
including;
methanolic
and
ultrasonic
of
Salvia
triloba
,
compare
their
phenolic
composition
utilizing
high‐performance
liquid
chromatograph
equipped
with
diode
array
detector
(HPLC‐DAD),
anti‐bacterial,
anti‐oxidant,
enzyme
inhibition
activities.
results
HPLC‐DAD
analysis
showed
that
Rosmarinic
acid
was
found
the
highest
amount
followed
by
extracts
as
169.7
±
0.51,
135.1
0.40,
28.58
0.46
μg/g
respectively.
Trans‐cinnamic
(4.40
0.09
μg/g)
exclusively
extract.
For
bioactivities,
exhibited
biofilm
against
Enterococcus
faecalis
compared
other
extracts,
while
outperformed
both
ultrasonic‐assisted
Staphylococcus
aureus
Escherichia
coli
strains
at
minimum
inhibitory
concentration.
considerable
quorum
sensing
Chromobacterium
violaceum
CV026,
no
activity
recorded
from
S.
moderate
butyrylcholinesterase
inhibition;
each
demonstrated
limited
effects
on
urease
enzyme.
Similarly,
significant
antioxidant
activity,
β‐carotene‐linoleic
assay
(IC
50
=
10.29
0.36
μg/mL),
DPPH
•
27.77
0.55
ABTS
•+
15.49
0.95
metal
chelating
57.80
CUPRAC
(assay
A
0.50
32.54
0.84
μg/mL).
Furthermore,
better
than
α‐tocopherol
(Standard
used).
potential
solvent(s)
eco‐friendly
alternative
for
extractin
compounds
evaluated
biological
activities
first
time.
Journal of Chemistry,
Год журнала:
2025,
Номер
2025(1)
Опубликована: Янв. 1, 2025
The
formation
of
biofilms
occurs
due
to
a
group
bacteria
gathering
together.
increasing
cell
density
will
stimulate
chemical
signals
for
communicate
through
quorum
sensing
system.
Quorum
plays
role
competition,
virulence,
resistance,
and
pathogenesis.
produces
signaling
response
called
as
autoinducers.
Gram‐negative
produce
N
‐acyl‐
L
‐homoserine
lactones
autoinducer,
while
in
Gram‐positive
autoinducing
peptides.
By
looking
at
responses
bacterial
pathogenesis
the
study
natural
antibiotic
agents
became
particular
concern
researchers.
This
review
summarizes
systems
acting
on
bacteria,
biofilm
by
pathogenic
potential
use
medicinal
plants
anti–quorum
reviewed
vitro
silico.
extracts
from
leaves,
fruits,
flowers,
stems,
isolated
compounds
some
types
essential
oils
has
been
successfully
tested
have
activity.
Frontiers in Chemistry,
Год журнала:
2025,
Номер
13
Опубликована: Март 11, 2025
Background
Camellia
sinensis
extracts
have
a
rich
phytochemical
profile
and
therapeutic
properties.
The
plant
contains
bioactive
compounds,
such
as
catechins,
flavonoids,
phenolic
acids,
which
are
associated
with
various
health
benefits,
including
antioxidant,
anti-inflammatory,
anticancer
activities.
Aim
To
investigate
the
potential
of
extract,
particularly
its
antibacterial
activity
against
Gram-positive
Gram-negative
bacteria
drug-like
Method
Phenolic
compounds
in
C.
extract
were
identified
quantified
using
high-performance
liquid
chromatography
(
HPLC
).
Its
was
assessed
both
Staphylococcus
aureus
)
Pseudomonas
aeruginosa
Escherichia
coli
Drug-likeness,
toxicity,
molecular
properties
investigated
computational
approaches.
Additionally,
binding
affinities
selected
predicted
via
docking
to
elucidate
mechanisms.
Results
caffeic
acid
(10.32
mg/g),
epigallocatechin
gallate
EGCG
,
8.74
syringic
(6.21
quercetin
(15.29
mg/g).
Antibacterial
testing
revealed
inhibition
zones
ranging
from
10.62
mm
for
E.
18.65
S.
comparable
gentamicin
(19.42
mm).
Molecular
that
(−9.8
kcal/mol)
most
potent
compound
P.
RNase
PH,
followed
by
(−8.7
kcal/mol).
Drug-likeness
modeling
indicated
favorable
profiles
although
violated
Lipinski’s
rule
due
weight
(458.4
g/mol).
Density
Functional
Theory
analysis
significant
variations
electronic
among
exhibiting
smallest
HOMO-LUMO
gap
(2.31
eV),
suggesting
high
reactivity.
MD
simulations
confirmed
stability
-protein
complex,
RMSD
values
(∼2.5–3.0
Å),
reduced
RMSF
at
key
residues,
stable
Rg
(∼18–20
Å).
Discussion
results
highlight
is
valuable
source
promising
bacteria,
.
Quercetin,
abundant
compound,
showed
better
chemical
(higher
gap),
but
lower
affinity
suggests
more
effective
candidate.
Moreover,
these
positions
them
alternatives
conventional
antibiotics.
Future
research
should
focus
on
vivo
validation,
structure-activity
optimization,
formulation
development
improve
bioavailability
clinical
applicability.
Antibiotics,
Год журнала:
2025,
Номер
14(4), С. 384 - 384
Опубликована: Апрель 5, 2025
Background/Objectives:
Wine
pomace
is
a
rich
source
of
bioactive
phenolic
compounds
with
potential
health
benefits.
This
study
aimed
to
evaluate
the
antipathogenic
and
antioxidant
properties
ethanol
ethyl
acetate
extracts
from
wine
three
grape
varietals
(Tannat,
Bonarda,
Malbec)
explore
their
as
natural
alternatives
for
mitigating
bacterial
virulence
in
Pseudomonas
aeruginosa.
Methods:
Successive
exhaustion
extractions
were
performed
using
solvents
increasing
polarity
(ethyl
ethanol).
The
content
was
quantified,
activity
evaluated
standard
assays.
against
P.
aeruginosa
assessed
by
measuring
biofilm
formation,
elastase
protease
activity,
pyocyanin
production,
swarming
motility.
Quorum
sensing
(QS)
inhibition
tested
violacein
production
assay
Chromobacterium
violaceum.
Results:
Ethanol
more
effective
at
extracting
compounds,
Tannat
exhibiting
highest
total
(162.5
µg
GAE/mg).
HPLC-DAD
analysis
identified
16
acids,
18
flavonoids,
3
stilbenes
across
extracts.
showed
strong
(phosphomolybdenum
reducing
capacity
67–128
μg
AAE/mg,
ABTS•+
scavenging
37–71
µg/mL,
Fe3+
power
31–68
µg/mL)
inhibited
formation
(up
61%),
41%),
46%)
activities
also
reduced
78%)
motility
68%),
suggesting
interference
QS.
Moreover,
C.
violaceum,
confirming
QS
26%).
Conclusions:
Among
extracts,
ethanol-extracted
most
substantial
activities.
results
add
value
its
use
capable
controlling
without
promoting
development
resistance.
Microorganisms,
Год журнала:
2025,
Номер
13(4), С. 843 - 843
Опубликована: Апрель 7, 2025
Antimicrobial
resistance
(AMR)
poses
a
global
health
threat,
with
multidrug-resistant
(MDR)
Klebsiella
pneumoniae
and
Pseudomonas
aeruginosa
contributing
to
the
burden,
especially
in
Bulgaria.
This
study
investigates
recent
trends
of
these
pathogens
Bulgaria
evaluates
antibacterial
effects
commercially
available
oregano
(Origanum
vulgare)
thyme
(Thymus
vulgaris)
essential
oils
(EOs),
both
alone
combination
conventional
antibiotics.
The
chemical
composition
EOs
was
analyzed
using
gas
chromatography–mass
spectrometry
(GC-MS),
revealing
carvacrol
(84.2%)
as
main
component
EO
thymol
(45.74%)
EO.
Minimum
inhibitory
concentration
(MIC)
minimum
bactericidal
(MBC)
values
were
determined
serial
dilution
method.
Oregano
exhibited
lower
MIC
MBC
(0.039–1.250%)
compared
(0.156–5.000%)
against
pathogens.
disk
diffusion
assay
showed
that
exhibits
more
potent
than
strains.
findings
highlight
potential
adjunctive
therapies
enhance
efficacy
antibiotics
MDR
strains
International Journal of Microbiology,
Год журнала:
2025,
Номер
2025(1)
Опубликована: Янв. 1, 2025
Multidrug‐resistant
(MDR)
Pseudomonas
aeruginosa
poses
a
global
challenge
due
to
its
high
virulence
and
resistance
mechanisms,
which
lead
persistent
infections.
This
study
explored
the
efficacy
of
active
compounds
Aloe
vera
as
alternative
treatments
against
MDR
P.
isolates.
A
total
283
isolates
were
obtained
from
sputum
throat
samples.
The
antibiotic
these
was
evaluated
several
classes.
minimum
inhibitory
concentration
(MIC)
A.
–derived
phytochemicals
individually
assessed;
lupeol
found
be
potent
phytochemical
with
lowest
MIC
(125
μ
g/mL).
physicochemical
properties
toxicity
further
using
SwissADME,
StopTox,
Protox
3.0.
Genetic
analysis
identified
mutations
in
AmpC
protein
key
factor
resistance;
consequently,
molecular
docking
studies
examined
interactions
between
AmpC.
Most
exhibited
pronounced
resistance,
while
demonstrated
favorable
pharmacological
strong
binding
amino
acids
Of
phytochemicals,
aloe‐emodin
showed
most
promising
antibacterial
activity.
These
findings
underscore
potential
for
clinical
application
combating
drug‐resistant
bacterial
