Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 21, 2024
Abstract
In
this
work,
we
compared
the
effects
of
probiotics
(Lactobacillus
reuteri
and
Lactobacillus
bulgaricus)
fermented
yogurt
on
chronic
unpredictable
mild
stress
(CUMS)-induced
anxious
depressive-like
symptoms
in
mice
as
well
discussed
potential
mechanism.
The
behavioral
test
results
showed
that
L.
bulgaricus
significantly
improved
anxious-
symptoms.
We
also
found
presented
an
overall
neuroprotective
effect
hippocampus
maintained
cerebral
activity.
yogurts
were
observed
to
ameliorate
levels
monoamine
neurotransmitters
inflammatory
cytokines
via
inhibiting
activation
NF-κB
pathway
accompanying
with
elevating
CREB-BDNF
pathway.
Our
findings
suggested
may
be
involved
modulating
gut
microbiota
metabolite
composition,
thereby
performing
ameliorating
depressive
behaviors
by
at
least
partially
microbiota-gut-brain
axis.
Pharmaceutical Biology,
Год журнала:
2025,
Номер
63(1), С. 156 - 169
Опубликована: Фев. 25, 2025
Context
Flavones,
the
key
active
components
in
Traditional
Chinese
Medicine
(TCM),
have
demonstrated
antidepressant
activity.
Given
numerous
animal
studies
conducted,
a
systematic
analysis
is
essential
to
provide
valuable
reference
for
future
research.
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Сен. 16, 2024
Introduction
Depression
is
a
common
psychological
disorder,
accompanied
by
disturbance
of
the
gut
microbiota
and
its
metabolites.
Recently,
microbiota-derived
tryptophan
metabolism
AMPK/mTOR
pathway
were
found
to
be
strongly
linked
development
depression.
Shugan
Hewei
Decoction
(SHD)
classical
anti-depression
traditional
Chinese
medicine
formula.
Although,
we
have
shown
that
SHD
exerted
antidepressant
effects
via
cecal
cecum
NLRP3
inflammasome,
specific
mechanism
on
driven
unknown.
In
this
study,
focus
elucidate
multifaceted
mechanisms
SHD.
Methods
Male
rats
established
chronic
unpredictable
stress
(CUS)/social
isolation
for
6
weeks,
SHD-L
(7.34
g/kg/d),
SHD-H
(14.68
Fructooligosaccharide
(FOS)
(3.15
g/kg/d)
given
intragastric
administration
once
daily
during
last
2
weeks.
Behavioral
experiments
carried
out
evaluate
model.
The
colonic
content
was
taken
shotgun
metagenomic
sequencing
combined
with
untargeted
metabolomics,
targeted
metabolomics.
ELISA
used
detect
levels
zonula
occludens
1
(ZO-1),
Occludin
in
colon,
as
well
lipopolysaccharide
(LPS),
diamine
oxidase
(DAO),
D-lactate
(DLA)
serum.
expressions
mRNA
proteins
adenosine
monophosphate-activated
protein
kinase
(AMPK)/mammalian
target
rapamycin
(mTOR)
autophagy
examined
using
RT-qPCR
Western
blot
colon.
Results
modulated
function
biological
pathways,
which
related
metabolism.
addition,
could
regulate
production
(such
reduction
3-HK,
3-HAA
etc.,
increment
ILA,
IAA
etc.),
metabolites
belong
kynurenine
(KYN)
indole
derivatives.
Further,
reduced
intestinal
permeability
enhanced
barrier
function.
Moreover,
upregulate
AMPK,
microtubule
associated
light
chain
3
(LC3),
5
(ATG5)
Beclin1,
downregulate
mTOR,
p62,
promoted
Spearman’s
analysis
illustrated
close
correlation
between
barrier,
pathway.
Conclusion
may
exert
antidepressant-like
regulating
metabolism,
triggering
autophagy,
enhancing
Translational Psychiatry,
Год журнала:
2025,
Номер
15(1)
Опубликована: Фев. 17, 2025
The
microbiota-gut-brain
axis
plays
a
pivotal
role
in
neuropsychiatric
disorders,
particularly
depression.
Escitalopram
(ESC)
is
first-line
antidepressant,
however,
its
regulatory
mechanisms
on
the
treatment
of
depression
remain
unclear.
antidepressant
effects
ESC
were
evaluated
using
forced
swim
test
Wistar-Kyoto
(WKY)
rats,
while
damage
gut
and
brain
regions
was
assessed
through
H&E
staining
immunohistochemistry.
therapeutic
WKY
rats
with
depression-like
behavior
investigated
16S
rRNA
sequencing
microbiota,
serum
untargeted
metabolomics,
hippocampal
proteomics.
Results
indicated
that
intervention
improved
depressive-like
behaviors,
as
evidenced
by
increased
swimming
times
also
restored
intestinal
permeability
tissue
integrity.
Significant
changes
microbiota
composition,
an
increase
Bacteroides
barnesiae,
well
increases
sphingolipid
metabolites
(Sphinganine
1-phosphate,
Sphingosine,
Sphingosine-1-phosphate)
proteins
(Sptlc1,
Enpp5,
Enpp2),
strongly
correlated.
These
robust
correlations
suggest
may
exert
modulating
metabolism
influence
microbiota.
Accordingly,
this
research
elucidates
novel
underlying
efficacy
highlights
importance
mediating
these
effects.
Neurobiology of Disease,
Год журнала:
2025,
Номер
207, С. 106852 - 106852
Опубликована: Фев. 20, 2025
Sphingolipids
are
a
class
of
lipids
highly
expressed
in
brain,
especially
the
myelin
sheath
white
matter.
In
recent
years,
with
development
lipidomics,
role
brain
sphingolipids
neurological
disorders
have
raised
lots
interests
due
to
their
function
neuronal
signal
transduction
and
survival.
Although
not
thoroughly
investigated,
some
previous
studies
indicated
that
homeostasis
closely
linked
etiology
disorders.
For
example,
disrupted
level
been
found
clinic
patients
disorders,
such
as
neurodegeneration
psychiatric
Conversely,
intervention
metabolism
by
modulating
activity
related
enzymes
also
could
result
pathological
deficits
identified
Moreover,
alteration
catabolic
pathway
be
partly
represented
cerebrospinal
fluid
blood
tissues,
which
show
diagnostic
potential
for
Therefore,
our
review
aims
summarize
discuss
known
contents
bioactive
sphingolipid
neurodegenerative
help
understand
mechanism
underlying
regulation
neural
provide
possible
directions
further
study.
The
new
perspectives
this
promising
field
will
open
up
therapeutic
options
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 24, 2025
Atherosclerosis
(AS)-related
cardiovascular
disease
and
depression
are
often
comorbid,
with
patients
facing
an
increased
risk
of
depression,
which
worsens
AS.
Both
diseases
characterized
by
oxidative
stress
lipid
metabolism
disorders.
Ferroptosis,
a
form
cell
death
iron
overload
harmful
peroxide
accumulation,
is
found
in
various
diseases,
including
AS
depression.
Consistent
the
deposition
peroxidation
(LPO)
that
characterize
ferroptosis
mechanism,
disturbances
also
crucial
pathogenic
mechanisms
The
comorbid
complex,
posing
challenges
for
clinical
treatment.
Chinese
herbs
hold
significant
potential
owing
to
their
multi-target
pharmacological
effects.
Therefore,
this
review
aims
investigate
overload,
LPO,
across
types,
shared
pathogenesis
ferroptosis,
research
on
herbal
medicine
targeting
treatment
anti-AS
co-depression.
This
provides
comprehensive
understanding
co-depression
from
perspective
ferroptosis.
Metabolites,
Год журнала:
2025,
Номер
15(3), С. 167 - 167
Опубликована: Март 1, 2025
Background:
Gut
microbiomes
play
a
vital
role
in
maintaining
whole-body
metabolic
homeostasis.
It
has
gained
significant
attention
recent
years
due
to
advancements
genome
sequencing
technologies
and
deeper
understanding
of
its
relationship
with
obesity.
However,
the
specific
ways
which
different
microorganisms
directly
or
indirectly
influence
host
obesity,
as
well
underlying
mechanisms,
remain
uncertain
because
complexity
gut
microbiota
composition.
Methods:
In
this
review,
we
summarize
roles
major
phyla
such
Bacteroidetes,
Firmicutes,
Proteobacteria,
Verrucomicrobia
obesity
type
2
diabetes
based
on
studies
published
past
five
PubMed
Google
Scholar.
The
current
therapeutic
strategies
associated
are
also
explored
from
clinical
trials,
challenges
future
directions
discussed.
Results
Conclusions:
This
review
will
provide
functions
diabetes,
could
lead
more
individualized
effective
treatments
for
diseases.
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Март 7, 2025
Background
and
Objective
Ischemic
stroke
is
a
cerebrovascular
disease
with
highly
incidence.
Previous
research
has
demonstrated
that
apigenin
provides
protective
effects
against
ischemic
stroke.
However,
it
remains
unclear
whether
can
regulate
intestinal
flora
Methods
In
this
study,
we
evaluated
the
regulatory
of
on
microbiota
using
middle
cerebral
artery
occlusion
rat
model.
The
impact
brain
damage
in
rats
was
assessed
through
Nissl
staining,
hematoxylin
eosin
immunohistochemistry.
Additionally,
employed
16S
rRNA
sequencing
to
analyze
contents
utilized
non-targeted
metabolomics
investigate
metabolites,
thereby
exploring
its
mechanism
action.
AMPK
levels
were
detected
by
Western
blot
kit
used
detect
oxidative
stress
inflammation.
Results
intervention
resulted
significant
alterations
flora,
characterized
an
increase
abundance
probiotic
species
decrease
harmful
alongside
notable
changes
metabolite
profiles.
This
effect
attributed
apigenin’s
promotion
expression
enhancement
energy
metabolism
context
addition,
improved
inflammation
Conclusion
These
findings
suggest
exerts
signaling
pathway
modulating
associated
metabolites.
Consequently,
emerges
as
therapeutic
candidate
warranting
further
investigation.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2722 - 2722
Опубликована: Март 18, 2025
Growing
neurochemical
evidence
highlights
cerebral
lipid
dysregulation
as
a
key
factor
in
the
pathophysiology
of
major
depressive
disorder
(MDD).
This
review
systematically
explores
dual
roles
species
both
normal
behavioral
regulation
and
MDD
development.
By
critically
examining
recent
literature,
we
classify
these
into
two
functional
categories
based
on
their
neuroactivity:
(1)
neuroprotective
lipids
(sphingomyelin,
cholesterol,
cardiolipin,
sphingosine,
phosphatidic
acid,
phosphatidylserine),
which
exert
effects
by
modulating
membrane
fluidity
supporting
synaptic
vesicle
trafficking;
(2)
neurotoxic
(ceramides,
phosphatidylinositol,
phosphocholine,
phosphatidylethanolamine),
promote
apoptotic
signaling
cascades
disrupt
mitochondrial
bioenergetics.
An
unresolved
but
critical
question
pertains
to
maintenance
homeostatic
equilibrium
between
opposing
classes.
balance
is
essential,
given
significant
impact
protein
localization
function,
monoaminergic
neurotransmitter
metabolism,
energy
homeostasis,
redox
neural
circuits
involved
mood
regulation.
emerging
framework
positions
lipidomics
promising
avenue
for
identifying
novel
therapeutic
targets
developing
biomarker-based
diagnostic
approaches
treatment.
