Ultra‐High Performance Liquid Chromatography/Triple Quadrupole Ion‐Trap Mass Spectrometry‐Based Quantitative Assay of 17 Compounds to Unveil the Extraction Difference of Potentially Toxic Components Between the Single Drugs and Sishen Formula DOI

M Wang,

Wandi Hu,

Jia Lou

и другие.

Separation Science Plus, Год журнала: 2024, Номер unknown

Опубликована: Дек. 2, 2024

ABSTRACT Sishen formula is a famous prescription utilized to treat kidney diarrhea. The main components of Psoraleae Fructus and Euodiae are reported with the liver toxicity; however, dissolution difference those toxic compounds among single medicine decoctions versatile compatibility forms has yet not been documented by quantitative assays. We established validated an ultra‐high performance liquid chromatography/triple quadrupole‐linear ion‐trap mass spectrometry‐multiple reaction monitoring approach targeting 17 (e.g., four coumarins eight flavonoids from Fructus; three alkaloids two limonoids Fructus). Co‐decoction Myristicae Semen or Schisandrae Chinensis could significantly affect Fructus, whereas weak influence was observed for combining Fructus. Combination increase contents both but effect relatively slight. Moreover, in remarkably inhibited, compared pure decoction, variation between decoction formula. These results benefit bridge efficacy toxicity changes

Язык: Английский

Euodiae Fructus: a review of botany, application, processing, phytochemistry, quality control, pharmacology, and toxicology DOI Creative Commons

Yule Hao,

Jiawen Qi,

Xinggui Huang

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Янв. 29, 2025

Euodiae Fructus (EF) is the dried and nearly ripe fruit of Euodia rutaecarpa , first recorded in Shen Nong’s Herbal Classic . EF a versatile Traditional Chinese Medicine (TCM) known for effects dispelling colds alleviating pain, suppressing adverse qi to relieve vomiting, boosting yang mitigate diarrhea. However, it should be noted that possesses mild toxicity. In TCM prescriptions, employed treat various ailments, including abdominal diarrhea, chronic non-atrophic gastritis, irritable bowel syndrome, primary dysmenorrhea. This review collected literature published before September 2024 on EF. An exhaustive analysis was conducted utilizing multiple sources, namely classic books scientific databases like Web Science, PubMed, Elsevier, ACS, ResearchGate, Google Scholar, National Knowledge Infrastructure. So far, more than 300 metabolites have been extracted identified from EF, exhibiting pharmacological effects, such as cardiovascular protection, gastrointestinal neuroprotection, anti-inflammation, analgesia, anti-tumor, glucose lipid metabolism regulation, etc. It also exhibits diverse toxicological properties poses specific toxic risks liver, heart, kidney. Nonetheless, research scarce regarding toxicology especially its cardiotoxicity nephrotoxicity. Further in-depth necessary explore mechanisms underlying EF’s develop strategies quality control toxicity mitigation. The can reduced by processing, but this aspect rarely discussed, needs further standardized. Evodiamine, rutaecarpine, limonin are effective one causes evodiamine rutaecarpine intensely studied, there few studies other Therefore, paper focuses botanical characteristics, traditional applications, processing methods, phytochemistry, control, pharmacology, We hope provides theoretical basis future high-value high-connotation development

Язык: Английский

Процитировано

0

Network Pharmacology and Experimental Validation Reveal Sishen Pill’s Efficacy in Treating NSAID-Induced Small Intestinal Ulcers DOI Creative Commons
Jiangtao Zhou,

Fengting Zhu,

Hong Liang

и другие.

Drug Design Development and Therapy, Год журнала: 2025, Номер Volume 19, С. 2035 - 2050

Опубликована: Март 1, 2025

Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used but often cause small intestinal ulcers (SIUs), for which effective therapies lacking. Sishen Pill (SSP), a traditional Chinese medicine, shows therapeutic promise, yet its mechanisms remain unclear. This study integrates network pharmacology, molecular docking, and experimental validation to systematically investigate SSP's protective against NSAID-induced SIUs. Active SSP ingredients were screened using the Traditional Medicine Systems Pharmacology (TCMSP) Encyclopedia of (ETCM) databases. SIU-related targets retrieved from GeneCards DisGeNET. Protein-protein interaction (PPI) networks constructed via STRING Cytoscape, followed by Gene Ontology (GO) Kyoto Genes Genomes (KEGG) pathway enrichment analyses. Molecular docking (AutoDock Vina, PyMOL) validated ligand-target interactions. In vivo employed an indomethacin-induced SIU rat model assess effects on ulcer severity, inflammation, oxidative stress, PI3K/AKT signaling. We identified 66 bioactive ingredients, 222 drug targets, 144 targets. revealed high binding affinity components (quercetin, bavachinin, rutaecarpine, evodiamine) key (AKT1, HSP90AA1, IL6, MAPK1, BCL2). KEGG analysis highlighted as central. vivo, reduced indices, suppressed pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), attenuated stress. also downregulated PI3K AKT1 mRNA expression, confirming modulation. elucidates multi-target mechanism SIUs, emphasizing role in suppressing These findings provide scientific foundation clinical application highlight potential safe, alternative conventional therapies.

Язык: Английский

Процитировано

0

ATF3 Restoration as a Potential Strategy in Managing Ulcerative Colitis: Implications from Sishen Pill Research DOI
Yutong Jin,

Xuetao Dong,

Weilong Zhong

и другие.

Phytomedicine, Год журнала: 2025, Номер unknown, С. 156814 - 156814

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Sishen Pills Inhibit Inflammatory Dendritic Cell Differentiation via miR-505-3p mediated E-cadherin Downregulation in Ulcerative Colitis DOI
Jiaqi Huang, Youbao Zhong, Nian Cheng

и другие.

Phytomedicine, Год журнала: 2024, Номер 135, С. 156035 - 156035

Опубликована: Сен. 19, 2024

Язык: Английский

Процитировано

3

Integrating UPLC-Q-Exactive Orbitrap/MS, Network pharmacology and experimental validation to reveal the potential mechanism of Kadsura cocicinea roots in Colon Cancer DOI
Shiqi Liu, Bingbing Shen, Huiying Li

и другие.

Journal of Ethnopharmacology, Год журнала: 2024, Номер 337, С. 118934 - 118934

Опубликована: Окт. 12, 2024

Язык: Английский

Процитировано

2

Traditional Chinese Medicine Formulas-Based Interventions on Colorectal Carcinoma Prevention: The Efficacies, Mechanisms and Advantages DOI Creative Commons

Xiaojuan Tang,

He Min,

Yuan Ren

и другие.

Journal of Ethnopharmacology, Год журнала: 2024, Номер unknown, С. 119008 - 119008

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

2

Ultra‐High Performance Liquid Chromatography/Triple Quadrupole Ion‐Trap Mass Spectrometry‐Based Quantitative Assay of 17 Compounds to Unveil the Extraction Difference of Potentially Toxic Components Between the Single Drugs and Sishen Formula DOI

M Wang,

Wandi Hu,

Jia Lou

и другие.

Separation Science Plus, Год журнала: 2024, Номер unknown

Опубликована: Дек. 2, 2024

ABSTRACT Sishen formula is a famous prescription utilized to treat kidney diarrhea. The main components of Psoraleae Fructus and Euodiae are reported with the liver toxicity; however, dissolution difference those toxic compounds among single medicine decoctions versatile compatibility forms has yet not been documented by quantitative assays. We established validated an ultra‐high performance liquid chromatography/triple quadrupole‐linear ion‐trap mass spectrometry‐multiple reaction monitoring approach targeting 17 (e.g., four coumarins eight flavonoids from Fructus; three alkaloids two limonoids Fructus). Co‐decoction Myristicae Semen or Schisandrae Chinensis could significantly affect Fructus, whereas weak influence was observed for combining Fructus. Combination increase contents both but effect relatively slight. Moreover, in remarkably inhibited, compared pure decoction, variation between decoction formula. These results benefit bridge efficacy toxicity changes

Язык: Английский

Процитировано

1