Uncovering proteome variations and concomitant quality changes of different drying methods Cordyceps sinensis by 4D-DIA structural proteomics

Frontiers in Nutrition, Год журнала: 2025, Номер 12

Опубликована: Фев. 5, 2025

Introduction Cordyceps sinensis is a fungus, serves dual purposes as both medicinal herb and food source. Due to its high water content, fresh difficult preserve, necessitating the drying necessary process . Methods Using 4D-DIA proteomics, researchers analyzed proteome profiles of (CK) under three different conditions: vacuum freeze-drying (FD), oven-drying (OD), air-drying (AD). In addition, it was found that protein free sulfhydryl content decreased significantly disulfide bond increased after methods. Results discussion A total 3762 proteins were identified, showing variations between groups content. control consisting samples methods, FD. vs CK exhibited fewest differentially abundant proteins, with majority being upregulated. On other hand, OD displayed greatest amount distinct significant rise in up-regulated down-regulated proteins. Analysis KEGG indicated predominantly concentrated pathways like ribosome, synthesis coenzymes, metabolism amino sugar nucleotide sugar. Notably, there overlap ribosome biogenesis eukaryotes pathways. The resulted upregulation expression linked various metabolic This observation suggests treatment might activate or enhance certain biochemical processes within organism, potentially influencing overall activity. finding highlights importance post-harvest dry methods on properties , which could have implications for nutritional value.This study provides theoretical basis realization resource utilization storage support guaranteeing sustainable development resources.

Язык: Английский

TMSB4X is a regulator of inflammation-associated ferroptosis, and promotes the proliferation, migration and invasion of hepatocellular carcinoma cells

Discover Oncology, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 18, 2024

Ferroptosis and inflammation are involved in cancer progression. The aim of this study was to identify inflammation-associated ferroptosis regulators hepatocellular carcinoma (HCC). FerrDb database searched for ferroptosis-related genes. RNA sequencing data clinicopathologic information HCC patients were downloaded from the Cancer Genome Atlas (TCGA) database. Weighted gene co-expression network analysis applied obtain genes probably ferroptosis. Univariate Cox regression conducted screen prognostic genes, 10 machine learning algorithms combined find optimal strategy evaluate prognosis based on prognosis-related divided into high risk group low group, differentially expressed obtained. Thymosin beta 4 X-linked (TMSB4X) overexpressed or knocked down cell lines, then qPCR, CCK-8, Transwell, flow cytometery assays performed detect change cells' phenotypes, Western blot used markers. 157 related obtained by WGCNA. rLasso algorithm, with highest C-index, screened out 29 hub model showed good efficacy predict patients. groups distinct molecular characteristics. TMSB4X most important which dominated classification, it highly samples. promoted viability, migration invasion, repressed cells. constructed is effective clinical outcome TMSB4X, ferroptosis, a potential biomarker therapeutic target HCC.

Язык: Английский

Comprehensive Prognostic and Immunoinfiltration Analysis in Esophageal Squamous Cell Carcinoma Patients Using a Combined EMT and Ferroptosis-Related Biomarker Signature

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

Background Limited research has been conducted on the interaction between ferroptosis and epithelial-mesenchymal transition (EMT) their combined effect esophageal squamous cell carcinoma (ESCC) patient prognosis. The present study aimed to develop a prognostic model based impact of EMT ESCC prognosis for clinical application. Methods Gene expression levels data patients were obtained from GSE53625 dataset in Expression Omnibus (GEO) database, Cancer Genome Atlas (TCGA) as validation set. By combining results Cox regression analysis least absolute shrinkage selection operator (LASSO) analysis, we selected nine genes associated with prognosis, which then used construct model. Immune infiltration was evaluated using CIBERSORT single-sample Set Enrichment Analysis methods. Finally, in vitro experiments assess oncogenic effects ACSL3 VIM. Results Nine key screened integrated score (FEIS). Compared low-FEIS group, high-FEIS group demonstrated shorter overall survival period. immune showed an increase elevated checkpoint molecules group. A nomogram constructed accurately predict Additionally, our confirmed Conclusion Our introduced novel tool that integrates -and EMT-related biomarker, offered valuable insights developing personalized treatment strategies patients.

Язык: Английский