Jiedu Huayu Extract Alleviate Acute Liver Failure via Promotion of GPX4 Expression and Inhibition of D-GalN/LPS-Induced Ferroptosis DOI Creative Commons
Yong Lin, Yong Du, Minggang Wang

и другие.

Natural Product Communications, Год журнала: 2024, Номер 19(12)

Опубликована: Дек. 1, 2024

Objective This study aims to explore the potential mechanisms of Jiedu Huayu granules (JDHY) mitigate D-galactosamine (D-GalN) and lipopolysaccharide (LPS)-induced acute liver failure (ALF) in a cell damage model. Methods ALF was modeled using various concentrations D-GalN + LPS. JDHY-medicated serum at different then co-cultured with model proportion. The best concentration time intervention were determined by Cell Counting Kit-8, Alanine Aminotransferase (ALT), Aspartate (AST). Western blot used assess expression Ferritin Heavy Chain 1 (FTH1), Transferrin Receptor 1(TfR1), Glutathione Peroxidase 4 (GPX4), Lysyl Oxidase (LOX), Prostaglandin-Endoperoxide Synthase 2(PTGS2). Malondialdehyde analyzed for lipid peroxidation, enzyme-linked immunosorbent assay detect glutathione, Tumor Necrosis Factor-alpha, Interleukin-10, Interleukin-6 expression, function indicators (ALT, AST). Additionally, GPX4 knocked down transfection, molecular JDHY treating explored through blot, PCR, assay. Results appropriate dose D-GalN/LPS-induced (10 mg/mL μg/mL LPS 48 h) optimal (15%) ALT, AST, Kit-8 assays. treatment reduced ALT AST levels, alleviated inhibited ferroptosis. mechanism involves enhancing antioxidant capacity cells increasing regulating ferroptosis proteins (downregulating TfR1, upregulating FTH1), inhibiting LOX PTGS2, suppressing inflammation Factor-alpha Interleukin-6, Interleukin-10). In addition, knockdown experiments revealed that knocking worsened ALF, while can alleviate promoting cells. Conclusion enhance reduce peroxidation affected protecting cell, alleviating inflammatory,

Язык: Английский

A new strategy for the treatment of intracerebral hemorrhage: Ferroptosis DOI

Ke Yao Sun,

Xin Yue Bai,

Lei Zhang

и другие.

Experimental Neurology, Год журнала: 2024, Номер 382, С. 114961 - 114961

Опубликована: Сен. 15, 2024

Язык: Английский

Процитировано

2
Ferroptosis in hepatocellular carcinoma: Mechanisms and therapeutic implications DOI Open Access

Shanjie Tu,

Yisheng Zou,

Meiqi Yang

и другие.

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 182, С. 117769 - 117769

Опубликована: Дек. 16, 2024

Язык: Английский

Процитировано

2
Mechanisms of Vitamins Inhibiting Ferroptosis DOI Creative Commons
Meng Zhang, Xin Chen,

Yumei Zhang

и другие.

Antioxidants, Год журнала: 2024, Номер 13(12), С. 1571 - 1571

Опубликована: Дек. 20, 2024

Ferroptosis is an iron-dependent form of cell death, which characterized by the uncontrolled and overwhelming peroxidation membrane lipids. has been implicated in progression various pathologies, including steatotic liver, heart failure, neurodegenerative diseases, diabetes. Targeted inhibition ferroptosis provides a promising strategy to treat ferroptosis-related diseases. Multivitamins, vitamins A, B, C, D, E, K, have shown good ability inhibit ferroptosis. For example, vitamin A significantly upregulated expression several key ferroptotic gatekeepers genes through nuclear retinoic acid receptors X (RAR/RXR). Vitamin B6 could compensate for impaired glutathione (GSH) levels restore Glutathione peroxidase 4 (GPX4) cells, ultimately inhibiting D up-regulate anti-ferroptosis proteins activating receptors. E hydroquinone K (VKH2) can directly propagation lipid peroxidation, thereby In this review, we summarize currently understood mechanisms provide reference information future research on development inhibitors.

Язык: Английский

Процитировано

1
Design and Synthesis of 1,4‐Diformyl‐Piperazine Ferrostatin‐1 Derivatives as Novel Ferroptosis Inhibitors DOI Open Access
Yifan Zhang, Wei Guo, Hui Zheng

и другие.

Chemical Biology & Drug Design, Год журнала: 2024, Номер 104(5)

Опубликована: Окт. 28, 2024

ABSTRACT The present study focuses on the design and synthesis of novel 1,4‐diformyl‐piperazine‐based ferrostatin‐1 (Fer‐1) derivatives, their evaluation against ferroptosis activity. synthesized compounds demonstrated significant anti‐ferroptosis activity in human umbilical vascular endothelial cells (HUVECs), with Compound 24 showing highest potency. Mechanistic studies revealed that effectively reduced intracellular reactive oxygen species (ROS) levels, mitigated mitochondrial damage, enhanced glutathione peroxidase 4 (GPX4) expression. Additionally, exhibited improved solubility plasma stability compared to control compounds, Fer‐1 JHL‐12. These findings suggest derivatives hold promise as therapeutic agents for ferroptosis‐associated cardiovascular diseases.

Язык: Английский

Процитировано

0
Discovery of phenazine derivatives as a new class of non-classical ferroptosis inhibitors and efficacy evaluation on a mouse model of liver injury DOI
Yunjie Wu, Yang Lu, Jing You

и другие.

European Journal of Medicinal Chemistry, Год журнала: 2024, Номер 282, С. 117042 - 117042

Опубликована: Ноя. 12, 2024

Язык: Английский

Процитировано

0
[Ferroptosis inducer Erastin inhibits proliferation of liver cancer cells DOI
Pan Zhao, Zihui Zhou, Liang Yu

и другие.

PubMed, Год журнала: 2024, Номер 44(11), С. 2131 - 2136

Опубликована: Ноя. 20, 2024

To investigate the expression of Acyl-CoA synthetase long-chain family member 4 (ACSL4) in liver cancer and its role regulating ferroptosis proliferation cells.

Язык: Английский

Процитировано

0
Jiedu Huayu Extract Alleviate Acute Liver Failure via Promotion of GPX4 Expression and Inhibition of D-GalN/LPS-Induced Ferroptosis DOI Creative Commons
Yong Lin, Yong Du, Minggang Wang

и другие.

Natural Product Communications, Год журнала: 2024, Номер 19(12)

Опубликована: Дек. 1, 2024

Objective This study aims to explore the potential mechanisms of Jiedu Huayu granules (JDHY) mitigate D-galactosamine (D-GalN) and lipopolysaccharide (LPS)-induced acute liver failure (ALF) in a cell damage model. Methods ALF was modeled using various concentrations D-GalN + LPS. JDHY-medicated serum at different then co-cultured with model proportion. The best concentration time intervention were determined by Cell Counting Kit-8, Alanine Aminotransferase (ALT), Aspartate (AST). Western blot used assess expression Ferritin Heavy Chain 1 (FTH1), Transferrin Receptor 1(TfR1), Glutathione Peroxidase 4 (GPX4), Lysyl Oxidase (LOX), Prostaglandin-Endoperoxide Synthase 2(PTGS2). Malondialdehyde analyzed for lipid peroxidation, enzyme-linked immunosorbent assay detect glutathione, Tumor Necrosis Factor-alpha, Interleukin-10, Interleukin-6 expression, function indicators (ALT, AST). Additionally, GPX4 knocked down transfection, molecular JDHY treating explored through blot, PCR, assay. Results appropriate dose D-GalN/LPS-induced (10 mg/mL μg/mL LPS 48 h) optimal (15%) ALT, AST, Kit-8 assays. treatment reduced ALT AST levels, alleviated inhibited ferroptosis. mechanism involves enhancing antioxidant capacity cells increasing regulating ferroptosis proteins (downregulating TfR1, upregulating FTH1), inhibiting LOX PTGS2, suppressing inflammation Factor-alpha Interleukin-6, Interleukin-10). In addition, knockdown experiments revealed that knocking worsened ALF, while can alleviate promoting cells. Conclusion enhance reduce peroxidation affected protecting cell, alleviating inflammatory,

Язык: Английский

Процитировано

0