Chaihuang Qingfu Pills Protect Against Acute Pancreatitis—Associated Acute Lung Injury Through MMP9-NLRP3-Pyroptosis Pathway

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 2317 - 2338

Опубликована: Фев. 1, 2025

Severe acute pancreatitis associated with lung injury (SAP-ALI) is a critical condition high mortality rate. Investigating the pathogenesis of SAP-ALI and developing effective treatments are urgently needed. Chaihuang Qingfu Pills (CHQF), traditional Chinese medicine modified from Qingyi Decoction, has been approved for treating (AP). However, its role in underlying mechanisms remain unclear. 92 AP patients were enrolled to observe protective effect CHQF on AP-ALI. L-arginine was used establish animal model. UHPLC-MS/MS identify components absorbed into serum. Transcriptomics analysis, network pharmacology, proteomics approaches explore molecular mechanism. In vivo vitro experiments conducted validate relevant findings. Clinical data indicated reduced incidence ALI 58.33% 36.36% patients. Animal demonstrated that decreased mortality, attenuated organ damage, inhibited systemic inflammation pathological SAP mice. Differential expression analysis weighted gene co-expression (WGCNA) identified 146 SAP-related differentially expressed genes (DEGs) GSE194331 dataset. acquired 26 blood 271 therapeutic targets. Integrated obtained 52 core targets SAP. Proteomic 216 proteins treatment SAP-ALI. Joint found MMP9 NLRP3 only common Both confirmed levels pyroptosis alveolar macrophages (AMs) under conditions. Moreover, inhibitor suppressed AMs pyroptosis. exerted by inhibiting macrophage through MMP9-NLRP3 pathway, providing novel strategy

Язык: Английский

Fuzheng Jiedu decoction alleviates H1N1 virus-induced acute lung injury in mice by suppressing the NLRP3 inflammasome activation

Journal of Ethnopharmacology, Год журнала: 2024, Номер unknown, С. 119314 - 119314

Опубликована: Дек. 1, 2024

Язык: Английский