[ DOI
Haonan Xu, Fang Zhang, Yuying Huang

и другие.

PubMed, Год журнала: 2025, Номер 45(2), С. 285 - 295

Опубликована: Фев. 20, 2025

To investigate the therapeutic mechanism of Thesium chinense Turcz. (TCT) for antibiotic-associated diarrhea (AAD). Network pharmacology, KEGG pathway enrichment analysis and molecular docking were used to identify shared targets genes TCT AAD, key signaling pathways binding between active components in core protein targets. In a Kunming mouse model AAD established by intragastric administration lincomycin hydrochloride, effects daily gavage 1% carboxymethyl cellulose sodium or gel solutions at 1.5 g/kg 3 (n=10) on body weight observed. HE staining, ELISA, 16S rRNA sequencing, Western blotting examine pathologies, expression levels IL-6 TNF-α, changes gut microbiota, expressions EGFR, p-EGFR, PI3K, p-PI3K, Akt, p-Akt colon tissues mice. We identified total 66 68 including STAT3 PIK3CA. suggested that was mediated primarily through PI3K/Akt pathway. Molecular showed EGFR had highest affinity with coniferin, EGFR-coniferin complex maintained stable conformation 10 ns, whose stability also confirmed Gibbs free energy analysis. models treatment significantly improved colonic tissue morphology, decreased TNF-α IL-6, increased microbiota diversity, modulated relative abundances genera Lactobacillus Bacteroides. markedly reduced p-PI3K can alleviate mice modulating composition, regulating EGFR/PI3K/Akt pathway, reducing TNF‑α expressions.

Язык: Английский

[ DOI
Haonan Xu, Fang Zhang, Yuying Huang

и другие.

PubMed, Год журнала: 2025, Номер 45(2), С. 285 - 295

Опубликована: Фев. 20, 2025

To investigate the therapeutic mechanism of Thesium chinense Turcz. (TCT) for antibiotic-associated diarrhea (AAD). Network pharmacology, KEGG pathway enrichment analysis and molecular docking were used to identify shared targets genes TCT AAD, key signaling pathways binding between active components in core protein targets. In a Kunming mouse model AAD established by intragastric administration lincomycin hydrochloride, effects daily gavage 1% carboxymethyl cellulose sodium or gel solutions at 1.5 g/kg 3 (n=10) on body weight observed. HE staining, ELISA, 16S rRNA sequencing, Western blotting examine pathologies, expression levels IL-6 TNF-α, changes gut microbiota, expressions EGFR, p-EGFR, PI3K, p-PI3K, Akt, p-Akt colon tissues mice. We identified total 66 68 including STAT3 PIK3CA. suggested that was mediated primarily through PI3K/Akt pathway. Molecular showed EGFR had highest affinity with coniferin, EGFR-coniferin complex maintained stable conformation 10 ns, whose stability also confirmed Gibbs free energy analysis. models treatment significantly improved colonic tissue morphology, decreased TNF-α IL-6, increased microbiota diversity, modulated relative abundances genera Lactobacillus Bacteroides. markedly reduced p-PI3K can alleviate mice modulating composition, regulating EGFR/PI3K/Akt pathway, reducing TNF‑α expressions.

Язык: Английский

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