Dysregulation of miR-106a-5p/PTEN axis associated with progression and diagnostic of postmenopausal osteoporosis DOI Creative Commons
Xiangjie Liu, Xiaogang Zhang,

M. Cen

и другие.

Journal of Orthopaedic Surgery and Research, Год журнала: 2025, Номер 20(1)

Опубликована: Май 13, 2025

Postmenopausal osteoporosis (PMOP) is a bone disorder in postmenopausal women and significant risk factor for fragility fractures. This study aims to explore the role of miR-106a-5p pathogenesis PMOP its potential as diagnostic biomarker. 220 were recruited. The levels miR-106a-5p, PTEN, osteogenic-related genes quantified using qRT-PCR. relative protein PTEN was detected Western blotting. ROC curve Pearson correlation employed evaluate value relationships between variables. To model iron accumulation, hFOB1.19 osteoblasts treated with ferric ammonium citrate (FAC). Cell proliferation apoptosis assessed CCK-8 flow cytometry. target relationship verified dual-luciferase assays. reduced, while increased PMOP. positively correlated mineral density negatively ferritin. In FAC-treated cells, decreased, increased. Dual-luciferase assays confirmed that targets PTEN. Successful transfection by observing corresponding changes expression. Up-regulated level, mRNA expression RUNX2, OPN, OCN, promoted cell proliferation, decreased under accumulation conditions. These effects reversed upregulation has diagnose linked ferritin levels. plays protective regulating conditions suggesting promising biomarker

Язык: Английский

Dysregulation of miR-106a-5p/PTEN axis associated with progression and diagnostic of postmenopausal osteoporosis DOI Creative Commons
Xiangjie Liu, Xiaogang Zhang,

M. Cen

и другие.

Journal of Orthopaedic Surgery and Research, Год журнала: 2025, Номер 20(1)

Опубликована: Май 13, 2025

Postmenopausal osteoporosis (PMOP) is a bone disorder in postmenopausal women and significant risk factor for fragility fractures. This study aims to explore the role of miR-106a-5p pathogenesis PMOP its potential as diagnostic biomarker. 220 were recruited. The levels miR-106a-5p, PTEN, osteogenic-related genes quantified using qRT-PCR. relative protein PTEN was detected Western blotting. ROC curve Pearson correlation employed evaluate value relationships between variables. To model iron accumulation, hFOB1.19 osteoblasts treated with ferric ammonium citrate (FAC). Cell proliferation apoptosis assessed CCK-8 flow cytometry. target relationship verified dual-luciferase assays. reduced, while increased PMOP. positively correlated mineral density negatively ferritin. In FAC-treated cells, decreased, increased. Dual-luciferase assays confirmed that targets PTEN. Successful transfection by observing corresponding changes expression. Up-regulated level, mRNA expression RUNX2, OPN, OCN, promoted cell proliferation, decreased under accumulation conditions. These effects reversed upregulation has diagnose linked ferritin levels. plays protective regulating conditions suggesting promising biomarker

Язык: Английский

Процитировано

0