Current Advances in the Therapeutic Potential of Scutellarin: Novel Applications, Mechanisms, and Future Challenges.
Phytomedicine Plus,
Год журнала:
2025,
Номер
unknown, С. 100754 - 100754
Опубликована: Янв. 1, 2025
Язык: Английский
Exploring the Mechanism of Wu-Mei-Wan in Treating Metabolic Syndrome Through Liver Autophagy Pathway Based on Bioinformatics and Omics Analysis
Опубликована: Янв. 1, 2025
Ethnopharmacological
relevance:Metabolic
syndrome
(MetS)
is
a
pathological
condition
characterized
by
obesity,
hyperglycemia,
hypertension,
and
hyperlipidemia,
which
increases
the
risk
of
cardiovascular
disease,
type
2
diabetes,
non-alcoholic
fatty
liver
disease.
The
traditional
Chinese
medicine
Wu-Mei-Wan
(WMW)
formula,
modified
from
Shanghanlun,
has
long
been
used
in
clinical
practice
for
gastrointestinal
metabolic
disorders.
However,
pharmacological
mechanisms
WMW
treatment
MetS
remain
unclear.Aim
study:
Here,
we
explored
actions
on
via
network
pharmacology
validated
mechanism
mice.Materials
methods:
A
involving
intersecting
genes
active
components
was
delineated.
top
proteins
underwent
molecular
docking.
And
findings
were
using
RT-PCR.
high
fat
diet
induced
mice
model
treated
with
WMW,
indicators
ELISA,
HE
staining,
oil
red
staining.
Metabolome
analysis
conducted
both
serum
feces
mice,
while
16S
microbiota
sequencing
specifically
performed
their
feces.
Ultimately,
comprehensive
omics
carried
out,
integrating
insights
metabolomic
analyses.Results:
study
revealed
that
improved
blood
biochemical
function
played
significant
role
protection.
Network
demonstrated
compounds
have
therapeutic
effects
modulating
lipids,
atherosclerosis.
Furthermore,
increased
expression
levels
PINK1
suggested
autophagy
exerts
protective
effect
syndrome.
Metabolomic
disruptions
pathways
involved
lipid
metabolism.
Joint
indicated
strong
correlation
between
these
factors.Conclusion:
In
this
can
enhance
pathway
activity
thereby
alleviating
protected
against
injury
through
oxidative
stress,
offering
new
into
its
potential.
Язык: Английский
Scutellarin ameliorates ischemia/reperfusion-mediated endothelial dysfunction by upregulating cathepsin D expression to rescue autophagy-lysosomal function
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Март 3, 2025
Background
Endothelial
dysfunction-induced
microcirculation
impairment
and
the
no-reflow
phenomenon
are
leading
causes
of
cardiac
ischemia/reperfusion
(I/R)
injury.
There
is
an
urgent
need
to
elucidate
underlying
mechanism
I/R-mediated
endothelial
dysfunction
identify
effective
drugs
for
treatment.
Scutellarin
(SCU),
a
flavonoid
compound,
has
been
extensively
studied
because
its
various
pharmacological
properties,
including
potent
protective
effects
on
cardiovascular
system.
However,
anti-endothelial
efficacy
mechanisms
action
SCU
have
not
investigated.
Approach
results
An
in
vivo
I/R
injury
model
was
established
using
coronary
artery
ligation
release.
oxygen-glucose
deprivation/oxygen-glucose
resupply
(OGD/OGR)
approach
used
establish
vitro
model.
We
evaluated
under
conditions,
both
.
pretreatment
promoted
vasodilation
reperfusion
blood
flow,
inhibited
myocardial
infarction,
improved
function
rats.
Additionally,
cell
membrane
damage,
reactive
oxygen
species
(ROS)
accumulation,
inflammation,
nitric
oxide
(NO)
reduction,
endothelin
1
(ET-1)
elevation
increase
expression
levels
vascular
growth
factor
(VEGF)
von
willebrand
(vWF)
cells.
Mechanistically,
rescued
lysosomal
flow
autophagic
flux
disrupted
by
through
upregulating
cathepsin
D
(CTSD)
levels.
Knockdown
CTSD
or
treatment
with
inhibitor
pepstatin
A
(P.A)
abrogated
cells
conditions.
Conclusion
demonstrated
that
SCU,
via
upregulation
cells,
autophagy-lysosomal
alleviated
dysfunction.
Thus,
potential
therapeutic
drug
prevention
Язык: Английский